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Plasmid#59028Purposeact as molecular scaffolds that mediate the activation of the JNK signaling pathway in vivo.DepositorInsertMAPK8IP2 mitogen-activated protein kinase 8 interacting protein 2 (MAPK8IP2 Human)
UseTagsT7ExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1 Srap1
Plasmid#89691PurposeExpresses murine Srap1 in bacteriaDepositorInsertMurine SRAP domain ortholog Srap1 (Hmces Mouse)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMTTH+eGFP-bromo5(N140A)
Plasmid#157806PurposeExpression of synthetic protein composed of eGFP and 5 repeats of BRD4 bromodomain #1 with N140A mutationDepositorInserteGFP-bromo5(N140A)
UseTagsHis6 and MBPExpressionBacterialMutationN140APromoterpTACAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET30a-SRAP-delCys2
Plasmid#84568PurposeEncodes murine SRAP domain (250aa) of Hmces/Srap1 with deletion of catalytic Cys2DepositorInsertSrap1 (Hmces Mouse)
UseTags6xHis and S-TagExpressionBacterialMutationdeletion of Cys2PromoterT7Available sinceNov. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET30a-SRAP
Plasmid#84524PurposeExpresses murine SRAP domain (250 aa) in bacteriaDepositorInsertSRAP domain (Hmces Mouse)
UseTags6xHis and S-TagExpressionBacterialMutationPromoterT7Available sinceNov. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB-eDIO-ChR2-EYFP
Plasmid#104387PurposePiggyBac plasmid encoding channelrhodopsin 2 fused to EYFP in an inverse orientation flanked by loxP and lox2722 sites under the control of the EF1a promotor.DepositorInsertChannelrhodopsin 2
UseCre/Lox; PiggybacTagsEYFPExpressionMammalianMutationE134RPromoterAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pKDsgRNA-pfkB
Plasmid#102284PurposeContains arabinose-induced lambda Red and a tet-inducible gRNA targeting pfkB.DepositorInsertpfkB gRNA
UseCRISPRTagsExpressionBacterialMutationPromoterpTetAvailable sinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEM-Cas9noSsrA
Plasmid#102285PurposeModified from pCas9-CR4 (Addgene: 62655) to remove the ssrA tag from Cas9.DepositorInsertCas9
UseCRISPRTagsExpressionBacterialMutationremoved ssrA tagPromoterpTetAvailable sinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pET28a-YJR129c
Plasmid#85118Purposebacterial expression of YJR129cDepositorInsertEFM3 (EFM3 Budding Yeast)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
Banshee-ShCit-A
Plasmid#85216PurposeShort hairpin RNAs were designed to target murine Cit (encoding Citron Rho-interacting kinase) and were subcloned into the Banshee-GFP vector for expression under control of the H1 promoter.DepositorInsertshCit-A
UseRetroviralTagsExpressionMammalianMutationPromoterH1Available sinceFeb. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
Banshee-ShCit-B
Plasmid#85217PurposeShort hairpin RNAs were designed to target murine Cit (encoding Citron Rho-interacting kinase) and were subcloned into the Banshee-GFP vector for expression under control of the H1 promoter.DepositorInsertshCit-B
UseRetroviralTagsExpressionMammalianMutationPromoterH1Available sinceFeb. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVRc27_4265
Plasmid#49745DepositorInsertAS27_4265
UseSynthetic BiologyTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpLuxAvailable sinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVRc14_1324
Plasmid#49734DepositorInsertAS14_1324
UseSynthetic BiologyTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpLuxAvailable sinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVRc38_1322
Plasmid#49752DepositorInsertAS38_1322
UseSynthetic BiologyTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpLuxAvailable sinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pSpliceExpress
Plasmid#32485DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDESTsplice
Plasmid#32484DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHBS1389 IBB-GFP-mCherry3E
Plasmid#118803PurposeFluorescence-based TDP43-dependent splicing reporterDepositorInsertSynthetic
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmVirD2
Plasmid#90314PurposemVirD2 expression plasmidDepositorInsertA. tumefaciens VirD2 from aa 1 to 204
UseTagsHis6x tagExpressionBacterialMutationPromoterT7/T5 IPTG inducibleAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
K84M kinase-dead Nuak1-3xflag
Plasmid#97219PurposePlasmid expressing kinase-dead mutant Nuak1DepositorInsertNuak1 (NUAK1 Human)
UseTagsFLAGExpressionBacterial and MammalianMutationKinase-dead K84MPromoterT7Available sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFS_0453_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)_T7term_ARRAY2_FaqI
Plasmid#117006PurposeExpresses E. coli codon-optimized FsRT-Cas1-Cas2 under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-Muc2D1D2D3cysD1
Plasmid#155214PurposeExpresses human MUC2 residues 21-1397DepositorInsertMuc2 D1D2D3cysD1 (MUC2 Human)
UseTags6xHistadineExpressionMutationPromoterAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
plenti2-EF1a-ChR2-EYFP-WRPE
Plasmid#125255Purpose3rd gen lentiviral expression of ChR2 with mutation fused to EYFP driven by EF1a promoter for optogenetic activationDepositorInserthChR2
UseLentiviralTagsEYFPExpressionMammalianMutationPromoterAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-Muc2D1D2D3
Plasmid#155215PurposeExpresses human MUC2 residues 21-1259DepositorInsertMuc2 D1D2D3 (MUC2 Human)
UseTags6xHistadineExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pCDNA3.1-Muc2D3
Plasmid#155216PurposeExpresses human Muc2 residues 858-1259DepositorInsertMuc2 D3 (MUC2 Human)
UseTags6xHistadineExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCWXPGR-pTF-dnTCF4
Plasmid#114277PurposeTET-inducible expression of a dominant negative form of the TCF4 transcription factorDepositorInsertTCF4 (TCF4 Human)
UseLentiviralTagsExpressionMutationdeletion of AA 1 to 31PromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
JA431
Plasmid#49937PurposeExpresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human KLF4 gene and demethylate CpGs adjacent to the binding siteDepositorInsertTet1CD KL-2 (KLF4 Human)
UseTALENTagsExpressionMutationPromoterEF1aAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
JA1229
Plasmid#49955PurposeExpresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding siteDepositorInsertTet1CD HB-2 (HBB Human)
UseTALENTagsExpressionMutationPromoterEF1aAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
hs-leonardo
Plasmid#42065DepositorInsertleonardo (14-3-3zeta Fly)
UseTagsExpressionInsectMutationPromoterHsp70Available sinceFeb. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUT1764-HQ858778
Plasmid#27860DepositorInsertPBF-2 like transcription factor from EE039788
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceMarch 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 (AAV1)
Viral Prep#101060-AAV1PurposeReady-to-use AAV1 particles produced from pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 (#101060). In addition to the viral particles, you will also receive purified pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 plasmid DNA. Synapsin-driven, photoconvertible (irreversible green-to-red) calcium integrator his-tagged CaMPARI2. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceJuly 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40 (AAV1)
Viral Prep#101061-AAV1PurposeReady-to-use AAV1 particles produced from pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40 (#101061). In addition to the viral particles, you will also receive purified pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40 plasmid DNA. Synapsin-driven, photoconvertible (irreversible green-to-red) calcium integrator his-tagged CaMPARI2-F391W. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceJuly 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40 (AAV1)
Viral Prep#101064-AAV1PurposeReady-to-use AAV1 particles produced from pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40 (#101064). In addition to the viral particles, you will also receive purified pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40 plasmid DNA. Synapsin-driven, photoconvertible (irreversible green-to-red) calcium integrator his-tagged CaMPARI2-L398T. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceJuly 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XFL-wtSrc
Plasmid#140295PurposeExpresses Flag-tagged wild-type human Src in mammalian cells for structure-function studiesDepositorInsertSrc wildtype (SRC Human)
UseTags2X FlagExpressionMammalianMutationPromoterCMVAvailable sinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pDEST40-2XFL-Src-Y530F
Plasmid#140319PurposeExpresses Flag-tagged human Src-Y530F in mammalian cells for structure-function studiesDepositorInsertSrc mutant (SRC Human)
UseTags2X FlagExpressionMammalianMutationY530FPromoterCMVAvailable sinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMTTH+H1.4
Plasmid#157796PurposeExpression of human linker histone H1.4DepositorInsertH1.4 (H1-4 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromoterpTACAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
DHB-mVenus
Plasmid#136461PurposeFluorescently tagged segment of DNA Helicase B that translocates from the nucleus to the cytoplasm in response to phosphorylation by CDK2DepositorInsertDNA Helicase B (HELB Human)
UseLentiviralTagsmVenusExpressionMutationPromoterAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
PH-Btk-GFP
Plasmid#51463PurposeIs a biosensor for PIP3DepositorInsertBTK (aa 1–177) (BTK Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEM-Cas9HF1
Plasmid#89961PurposeModified from pCas9-CR4 (Addgene: 62655) to use the high-fidelity version of Cas9, SpCas9-HF1 (N497A/R661A/Q695A/Q926A) from Kleinstiver et al 2016.DepositorInsertCas9HF1
UseTagsExpressionBacterialMutationN497A/R661A/Q695A/Q926APromoterpTetAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHAGE DN Cullin5
Plasmid#41916DepositorInsertCUL5 (CUL5 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 3, 2013AvailabilityAcademic Institutions and Nonprofits only