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Showing: 1001 - 1050 of 15387 results

  3. pE_Cre_lambdaCos

    Plasmid
    #173785
    Purpose
    The pE_Cre_lambdaCos plasmid is used to remove the antibiotic resistance cassette from pIT5 integrants.
    Depositor
    Keith Shearwin
    Article
    Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102
    Insert
    Cre
    Use
    Cre/Lox and Synthetic Biology
    Tags
    Expression
    Mutation
    S102Y and S279Y- please see depositor comments
    Promoter
    Available since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    173785-plasmid-map-sequence-id-342369

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  11. ACE2 g3

    Plasmid
    #153013
    Purpose
    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry
    Depositor
    Jason Sheltzer
    Insert
    ACE2 (ACE2 Human)
    Use
    CRISPR and Lentiviral
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Available since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    153013-plasmid-map-sequence-id-298679

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  14. pAS007

    Plasmid
    #164868
    Purpose
    This plasmid can be used to study DNA looping in vivo. pAS007 contains the phiKO2 cB operator sequences but can be modified to insert any DNA operators of interest.
    Depositor
    Keith Shearwin
    Article
    Hao et al Nucleic Acids Res. 2021 Jan 28. pii: 612
    Insert
    phiKO2 operators
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    164868-plasmid-map-sequence-id-325280

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  30. relAE335Q_NS1

    Plasmid
    #100917
    Purpose
    expresses E.coli relAE335Q from the NS1 site in the S. elongatus PCC7942 genome
    Depositor
    Erin O'Shea
    Article
    Puszynska et al Cell Rep. 2017 Dec 12;21(11):3155-3165.
    Insert
    RelA fragment from E.coli with point mutation E335Q
    Use
    Cyanobacteria cloning vector
    Tags
    FLAG
    Expression
    Mutation
    E 335 converted to Q
    Promoter
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    100917-plasmid-map-sequence-id-196978

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  47. pLenti-dCas9-KRAB-gRNA-TRE-blast

    Plasmid
    #201152
    Purpose
    Lentiviral expression of S. pyogenes dead Cas9 (dCas9/dSpCas9/SpdCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.
    Depositor
    Moshe Szyf
    Inserts
    dCas9-KRAB
    gRNA-TRE
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mutation
    gRNA sequence: TACGTTCTCTATCACTGAT
    Promoter
    Available since
    July 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    201152-plasmid-map-sequence-id-401799

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Showing: 1001 - 1050 of 15387 results