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We narrowed to 1,731 results for: plasmids pcas

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Showing: 401 - 420 of 1731 results

  2. pCMV-T7-SpCas9-VRER-P2A-mScarlet (MNW003)

    Plasmid
    #174138
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    VRER=D1135V/G1218R/R1335E/T1337R
    Promoter
    CMV and T7
    Available Since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174138-plasmid-map-sequence-id-341915

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  3. pCMV-T7-SpCas9-VRQR-P2A-mScarlet (MNW004)

    Plasmid
    #174139
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    VRQR=D1135V/G1218R/R1335Q/T1337R
    Promoter
    CMV and T7
    Available Since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174139-plasmid-map-sequence-id-341913

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  5. pCAGGS-SARS2-S-G614 (C-flag)

    Plasmid
    #156421
    Purpose
    Mammalian expression of SARS-CoV-2 S protein (G614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.
    Depositor
    Hyeryun Choe, Michael Farzan
    Article
    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:
    Insert
    SARS-CoV-2 S protein (S SARS CoV2)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    D614G
    Promoter
    CMV
    Available Since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    156421-plasmid-map-sequence-id-307829

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  7. pCAGGS-SARS2-S-D614 (NC-flag)

    Plasmid
    #156418
    Purpose
    Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.
    Depositor
    Hyeryun Choe, Michael Farzan
    Article
    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:
    Insert
    SARS-CoV-2 S protein (S SARS CoV2)
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    156418-plasmid-map-sequence-id-307819

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  10. pCAGGS-SARS2-S-G614 (NC-flag)

    Plasmid
    #156419
    Purpose
    Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.
    Depositor
    Hyeryun Choe, Michael Farzan
    Article
    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:
    Insert
    SARS-CoV-2 S protein (S SARS CoV2)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    D614G
    Promoter
    CMV
    Available Since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    156419-plasmid-map-sequence-id-308855

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  11. pCAG-eCas9-GFP-U6-gRNA

    Plasmid
    #79145
    Purpose
    All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in human pluripotent stem cells
    Depositor
    Jizhong Zou
    Insert
    eSpCas9(1.1)
    Use
    CRISPR
    Tags
    2A-GFP
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    June 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    79145-plasmid-map-sequence-id-182555

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  12. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)

    Plasmid
    #42335
    Purpose
    A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.
    Depositor
    Feng Zhang
    Article
    Cong et al Science. 2013 Jan 3.
    Has Service
    Cloning Grade DNA
    Insert
    humanized S. pyogenes Cas9 (D10A) nickase
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D10A nickase-converting mutation
    Available Since
    Feb. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    42335-plasmid-map-sequence-id-275689

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Showing: 401 - 420 of 1731 results