We narrowed to 14,531 results for: TIM
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Plasmid#84292PurposeExpress LdPBK_241510.1 and LdMT targeting gRNAs simutaneouslyDepositorInsertLdBPK_241510.1 targeting gRNA and LdMT targeting gRNA
UseCRISPR; Leishmania donovaniPromoterL. donovani ribosome RNA promoterAvailable SinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBabe RFP1-Smad2 neo
Plasmid#58491Purposemammalian expression of mRFP1-tagged mouse Smad2DepositorAvailable SinceAug. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-cBEST-v2-PermE
Plasmid#209446Purposeimproved pCRISPR-cBEST plasmid with optimized sgRNA cloning siteDepositorInsertCodon optimized APOBEC1-nCas9-UGI fusion protein
UseCRISPR and Synthetic BiologyPromoterPtipA; sgRNA expression from ermE* promoterAvailable SinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-shCTRL
Plasmid#181875PurposeExpresses a control shRNA with no known targets in the mouse genome under control of the U6 promoter and mCherry under control of the CaMKIIa promoterDepositorInsertcontrol shRNA with no known targets in the mouse genome
UseAAV, Adenoviral, and Mouse TargetingTagsmCherryExpressionMammalianPromoterU6Available SinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRN3P-HA-Suv4-20h1
Plasmid#86689PurposeFor transcription of of Suv4-20h1 mRNA preceded by an HA-tag in 5'DepositorAvailable SinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
SWEET11:SWEET11-2A-GFP-GUS
Plasmid#172484PurposeTranslational fusion of SWEET11 with a P2A self cleaving peptide sequenceDepositorAvailable SinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
FKBP-CD28TMD-NTEVp(H75E)
Plasmid#137829PurposeMESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75E mutant TEV proteaseDepositorInsertFKBP-CD28TMD-NTEVp(H75E)
Tags3x FLAGExpressionMammalianMutationMutated Histidine 75 to Glutamic Acid in NTEVp fr…PromoterCMVAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-mTagBFP2-2A-Puro_hU6-RfxDR36-BsmBI
Plasmid#226011PurposeCasRx guide RNA cloning backbone (lenti)DepositorTypeEmpty backboneUseLentiviralAvailable SinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDIV303
Plasmid#204978PurposeAMA1 plasmid with Aspergillus optimized Mad7 and amdS selection markerDepositorInsertsMad7
amdS
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationtwo silent mutations (nt177T>C, nt879C>T)PromoterAspergillus nidulans tef1 promoter and Aspergillu…Available SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Twitch-3 pcDNA3
Plasmid#49532PurposeFluorescent reporter for calcium signalingDepositorInsertTwitch-3
TagsECFP and cpCit174ExpressionMammalianPromoterCMVAvailable SinceJan. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMY-MCS-P2A-CD90.2
Plasmid#163355PurposeRetroviral backbone for in-frame cloning of a gene of interest and a P2A separated CD90.2 surface markerDepositorTypeEmpty backboneUseRetroviralExpressionMammalianAvailable SinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCyRFP1-RhoA
Plasmid#84358Purposefusion protein of RhoA, FRET donorDepositorInsertmCyRFP1-RhoA (RHOA Human, Synthetic)
TagsmCyRFP1 N terminal fusion to RhoAExpressionMammalianPromoterCMVAvailable SinceNov. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-GtACR1_C102A-EYFP
Plasmid#119196PurposeStep-function mutant of Guillardia theta anion channelrhodopsin 1 (GtACR1) with dramatically prolonged open stateDepositorInsertAnion channelrhodopsin GtACR1 step-function mutant C102A
UseLentiviralTagsEYFPMutationchanged cysteine 102 to alaninePromoterUbiquitinAvailable SinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA16_Efa-NLS(SV40) (KAC459)
Plasmid#134337PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIA16_Efa with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIA16_Efa
TagsNLS(SV40)ExpressionMammalianPromoterCMV and T7Available SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-hAcrVA1-NLS(sv40) (BPK5050)
Plasmid#115136PurposeCMV-T7 promoter expression plasmid for human codon optimized AcrVA1 anti-CRISPR protein with C-terminal NLSDepositorInserthuman codon optimized AcrVA1 anti-CRISPR protein
TagsNLS(SV40)ExpressionMammalianAvailable SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-MCU
Plasmid#31726DepositorAvailable SinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA
Plasmid#68719PurposeCre-dependent bicistronic vector expressing mRuby2 and GCaMP6f from a single open reading frame.DepositorHas ServiceAAV1InsertmRuby2-P2A-GCaMP6f
UseAAVPromoterCAG-FLEXAvailable SinceJune 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGB1alpha11_SulR
Plasmid#186424Purposetranscriptional unit for sulfadiazine resistance; plant expression driven by the Pnos promoter; suitable for quintuple assemblyDepositorInsertSulR
UseSynthetic Biology; Binary vector for escherichia …ExpressionPlantMutationBsaI and BsmBI sites removedPromoterPnosAvailable SinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOGG043
Plasmid#133123PurposeLevel 0 PU module synthesised as a fragment (pL0M-PU-PsNifH). Synthetic consensus promoter to drive nodule-specific expression in biovars and strains of R. leguminosarum.DepositorInsertPsNifH Synthetic consensus nifH promoter
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-NIrD
Plasmid#65010PurposeNS3/4A Inducible rtTA-mediated Dual-reporter system for Hepatitis C Virus (HCV) infectionDepositorInsertsrtTA-MAVS(C)
2A-linked delta-TK
UseLentiviralTagsmCherryExpressionMammalianPromoterTight-TREAvailable SinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only