We narrowed to 80,372 results for: TRI

1. pCX-DI-2A

   Plasmid

   #74671

   Purpose

   Mammalian expression of DI-2A (hCD73/F2A/hCD39)

   Depositor

   Roberto Giovannoni

   Article

   De Giorgi et al Nucleosides Nucleotides Nucleic Acids. 2

   Inserts

   CD73 (NT5E Human)
   CD39 (ENTPD1 Human)
   

   Use

   Tags

   F2A

   Expression

   Mammalian

   Mutation

   Promoter

   pCAGGS

   Available since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-nEF-Coff/Fon-iC++-EYFP

   Plasmid

   #137157

   Purpose

   Intersectional viral expression of iC++-EYFP in cells expressing Flp AND NOT Cre

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Coff/Fon-iC++-EYFP
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   n/a

   Promoter

   nEF

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX-EF1α-BHLHE40-IRES-ZsGreen1

   Plasmid

   #153061

   Purpose

   Lentiviral vector expressing human BHLHE40

   Depositor

   Silvia Monticelli

   Article

   Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

   Insert

   BHLHE40 (BHLHE40 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Parvalbumin scFv [L114/3]

   Plasmid

   #190504

   Purpose

   Mammalian Expression of Parvalbumin scFV. Derived from hybridoma L114/3.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Parvalbumin (Rattus norvegicus) recombinant scFV (Pvalb Mouse)
   

   Use

   Tags

   HA, 6xHis, Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. single polypeptide FPX biosensor for caspase-3

   Plasmid

   #60883

   Purpose

   FPX biosensor, a new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization dependent fluorescent proteins (ddFPs).

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   single polypeptide FPX biosensor for caspase-3
   

   Use

   Tags

   HindIII site and NES sequence LALKLAGLDIGS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. VGlut2 scFv [N29/29]

   Plasmid

   #182085

   Purpose

   Mammalian Expression of VGlut2 scFv. Derived from hybridoma N29/29.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   recombinant mouse scFv targeting VGlut2 (Rattus norvegicus) (Slc17a6 Mouse)
   

   Use

   Tags

   HA, His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. GIV-WT - FLAG

   Plasmid

   #65947

   Purpose

   Expression GIV/Girdin in Mammalian cell

   Depositor

   Pradipta Ghosh

   Article

   Lin et al Mol Biol Cell. 2014 Nov 5;25(22):3654-71

   Insert

   GIV/Girdin (CCDC88A Human)
   

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pALS2-sfGFP 150TAG

   Plasmid

   #197574

   Purpose

   Fluorescence selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-150TAG and M. alvus Pyl-tRNA(6). p15a origin of replication.

   Depositor

   Ryan Mehl

   Article

   Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

   Inserts

   sfGFP 150TAG
   M. alvus Pyl-tRNA (6)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   N150TAG

   Promoter

   araC and lpp

   Available since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. IC65 pEGFPN1 anti-IRSp53 nanobody

   Plasmid

   #135227

   Purpose

   Mammalian expression of anti-IRSp53 (Human) recombinant llama nanobody for use as an intrabody

   Depositor

   James Trimmer

   Article

   Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

   Insert

   anti-IRSp53 (Human) recombinant llama nanobody IC65 (BAIAP2 L. glama (llama))
   

   Use

   Tags

   6xHis-HA-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. SPHKAP scFv [L131/17]

    Plasmid

    #190511

    Purpose

    Mammalian Expression of SPHKAP scFV. Derived from hybridoma L131/17.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    SPHKAP (Mus musculus) recombinant scFV (Sphkap Mouse)
    

    Use

    Tags

    HA, Sortase, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIRES2-EGFP-deltaNp53:WTp53

    Plasmid

    #49244

    Purpose

    expresses human p53 deltaN linked to full length p53 with C terminal His tag and also expresses EGFP in mammalian cells

    Depositor

    Dylan Taatjes

    Article

    Lin et al Aging Cell. 2013 Oct;12(5):863-72. doi:

    Insert

    p53 deltaN and p53 WT (TP53 Human)
    

    Use

    Tags

    His and IRES-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. Parvalbumin scFv [L114/38]

    Plasmid

    #206777

    Purpose

    Mammalian Expression of Parvalbumin scFV. Derived from hybridoma L114/38 scFv.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Parvalbumin (Rattus norvegicus) recombinant scFV (Pvalb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pThermoCas9_ctrl

    Plasmid

    #100981

    Purpose

    Expresses ThermoCas9 and its sgRNA module

    Depositor

    John van der Oost

    Article

    Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:

    Inserts

    Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
    ThermoCas9 single guide RNA expressing module
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    B. coagulans DSM 1 pta promoter and B. smithii xy…

    Available since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. p5E-Runx1+23

    Plasmid

    #69602

    Purpose

    Runx1 +23 kb intronic enhancer from mouse and beta-globin minimal promoter in a 5' entry vector for gateway cloning

    Depositor

    Owen Tamplin, Leonard Zon

    Article

    Tamplin et al Cell. 2015 Jan 15;160(1-2):241-52. doi:

    Inserts

    Runx1 +23 intronic enhancer (Runx1 Mouse)
    Mouse beta-globin minimal promoter
    

    Use

    5' gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    C57/BL6 mouse Runx1 +23 intronic enhancer and C57…

    Available since

    Sept. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. LENTICRISPR-ALDOA_sgRNA1

    Plasmid

    #201590

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    ALDOA (ALDOA Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pan-Synapsin scFv [L125/129]

    Plasmid

    #182079

    Purpose

    Mammalian Expression of pan-Synapsin scFv. Derived from hybridoma L125/129.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    recombinant mouse scFv targeting pan-Synapsin
    

    Use

    Tags

    Sortase, His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAcBac1-Mb-sfGFP WT

    Plasmid

    #197569

    Purpose

    Expression of sfGFP WT with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    sfGFP WT
    Pyl-tRNA (4 copies)
    

    Use

    Tags

    V5-His6

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6/H1

    Available since

    April 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. hHSF1 RNAi resistant

    Plasmid

    #118347

    Purpose

    This plasmid expresses RNAi resistance version of hHSF1 to use for rescue experiments with HSF1 shRNA vector (plasmid #118345).

    Depositor

    Lea Sistonen

    Article

    Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

    Insert

    hHSF1 (HSF1 Human)
    

    Use

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    RNAi resistance mutations (does not change amino …

    Promoter

    Available since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. GST-Hsp90 N(9-236)

    Plasmid

    #22481

    Depositor

    William Sessa

    Article

    Fontana et al Circ Res. 2002 May 3. 90(8):866-73.

    Insert

    Hsp90 alpha N domain (HSP90AA1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-Ef1a-Con/Foff 2.0-EYFP

    Plasmid

    #137162

    Purpose

    Intersectional viral expression of EYFP in cells expressing Cre AND NOT Flp

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Foff 2.0-EYFP
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    n/a

    Promoter

    EF1a

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pOPINB-hRNF216(457-784)

    Plasmid

    #171922

    Purpose

    Expression of human RNF216 (Triad3A) codon optimized for E. coli and insect cells. Catalytic helix-RBR-helix construct suitable for activity assays.

    Depositor

    Bernhard Lechtenberg

    Article

    Cotton et al Mol Cell. 2022 Feb 3;82(3):598-615.e8. d

    Insert

    E3 ubiquitin-protein ligase RNF216 (RNF216 Human)
    

    Use

    Tags

    3C protease cleavage site and 6x-His tag

    Expression

    Bacterial

    Mutation

    codon optimised for expression in E. coli

    Promoter

    T7

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. pcDNA3 MLL2 653

    Plasmid

    #11017

    Depositor

    Matthew Meyerson

    Article

    Hughes et al Mol Cell. 2004 Feb 27. 13(4):587-97.

    Insert

    MLL2 (KMT2B Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    truncation of MLL2, last 653 amino acids, from pr…

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

23. pMT-sox10-NLS-BirA-2A-mCherry_Ras

    Plasmid

    #80063

    Purpose

    Sox10 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Sox10 promoter

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-nEF-Con/Fon/Von eYFP

    Plasmid

    #137164

    Purpose

    Intersectional viral expression of EYFP in cells expressing Cre AND Flp AND Vcre

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV Retrograde and AAV8

    Insert

    3x-EYFP
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    n/a

    Promoter

    nEF

    Available since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-nEF-Con/Fon-iC++-EYFP

    Plasmid

    #137155

    Purpose

    Intersectional viral expression of iC++-EYFP in cells expressing both Cre AND Flp

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Fon-iC++-EYFP
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    n/a

    Promoter

    nEF

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1+FLAG-KrasnatG12D

    Plasmid

    #206842

    Purpose

    To express mouse Kras encoded by native mouse codons and with a G12D mutation with an N-terminal FLAG tag.

    Depositor

    Christopher Counter

    Article

    Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

    Insert

    Kras (Kras Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Glycine 12 to Aspartic Acid

    Promoter

    Available since

    Oct. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDB-His-MBP-mGSDMD

    Plasmid

    #123365

    Purpose

    Expression of full-length mouse GSDMD in E. coli

    Depositor

    Hao Wu

    Article

    Orning et al Science. 2018 Nov 30;362(6418):1064-1069

    Insert

    GSDMD (Gsdmd Mouse)
    

    Use

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. BII-BCA-Prom-ASCL2

    Plasmid

    #133394

    Purpose

    Piggybac vector for heterologous promoter reporter assay of human ASCL2 promoter

    Depositor

    Blair Madison

    Article

    Strubberg et al Stem Cell Reports. 2018 Aug 14;11(2):410

    Insert

    destabilized tdTomato-NLS and destabilized NLS-GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free) drives expressi…

    Available since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. GC52 pEGFPN1 anti-Gephyrin nanobody

    Plasmid

    #135224

    Purpose

    Mammalian expression of anti-Gephyrin (Human) recombinant llama nanobody for use as an intrabody

    Depositor

    James Trimmer

    Article

    Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

    Insert

    anti-Gephyrin (Human) recombinant llama nanobody GC52 (GPHN L. glama (llama))
    

    Use

    Tags

    6xHis-HA-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. R620-M11-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6

    Plasmid

    #170195

    Purpose

    Mammalian expression of SARS-CoV-2 soluble spike trimer protein

    Depositor

    Dominic Esposito

    Article

    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

    Insert

    SARS-CoV-2 S (spike) (S SARS-CoV-2)
    

    Use

    Tags

    His6

    Expression

    Mammalian

    Mutation

    Promoter

    CMV51

    Available since

    Nov. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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