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DKC1 F1.3 gRNA

Plasmid

#90652

Purpose

3rd generation lentiviral gRNA plasmid targeting human DKC1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

DKC1 (Guide Designation F1.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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DKC1 F2.3 gRNA

Plasmid

#90653

Purpose

3rd generation lentiviral gRNA plasmid targeting human DKC1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

DKC1 (Guide Designation F2.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-ExRai-AKAR2

Plasmid

#161753

Purpose

Enhanced excitation-ratiometric biosensor for monitoring Protein Kinase A activity in living cells and in vivo.

Depositor

Jin Zhang

Article

Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

Insert

ExRai-AKAR2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe puro HA PIK3CA E545K

Plasmid

#12525

Depositor

Jean Zhao

Article

Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

Insert

PIK3CA E545K (PIK3CA Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

E545K. Tumor-derived allele.

Available Since

Aug. 18, 2006

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-MKLP1

Plasmid

#70145

Purpose

mammalian expression of MKLP1 with an eGFP fusion

Depositor

Masanori Mishima

Article

Douglas et al Curr Biol. 2010 May 25;20(10):927-33. do

Insert

MKLP1 (KIF23 Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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PGK-H2BeGFP

Plasmid

#21210

Depositor

Mark Mercola

Article

Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

Insert

Phosphoglycerate kinase promoter (PGK1 Human)

Use

Lentiviral

Tags

H2B-eGFP

Available Since

July 1, 2009

Availability

Academic Institutions and Nonprofits only

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PGK-H2BmCherry

Plasmid

#21217

Depositor

Mark Mercola

Article

Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

Insert

Phosphoglycerate kinase promoter (PGK1 Human)

Use

Lentiviral

Tags

H2B-mCherry

Available Since

July 1, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti6-CENP-A-mCherry

Plasmid

#89767

Purpose

Lentivirus destination vector for stable expression of mCherry-tagged kinetochore marker CENP-A

Depositor

Torsten Wittmann

Article

Pemble et al J Cell Sci. 2017 Apr 15;130(8):1404-1412

Insert

CENP-A (CENPA Human)

Use

Lentiviral

Tags

3xHA and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pWPI-GFP-3xFLAG

Plasmid

#201639

Purpose

Over-expression of 3xFLAG-tagged GFP

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

GFP

Use

Lentiviral

Tags

3xFLAG

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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PIK3CA - Exon20 H1047R

Plasmid

#16639

Depositor

Bert Vogelstein

Article

Samuels et al Science. 2004 Apr 23. 304(5670):554.

Insert

PIK3CA H1047R (PIK3CA Human)

Tags

Flag

Expression

Mammalian

Mutation

H1047R

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only

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FOXM1 C11.2 gRNA

Plasmid

#90690

Purpose

3rd generation lentiviral gRNA plasmid targeting human FOXM1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

FOXM1 (Guide Designation C11.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pZW6 (CK2alpha)

Plasmid

#27086

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2alpha (CSNK2A1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pcdna3 Flag JIP3b

Plasmid

#53458

Purpose

interacts with MAPK signaling module

Depositor

Roger Davis

Article

Kelkar et al Mol Cell Biol. 2000 Feb;20(3):1030-43.

Insert

mitogen-activated protein kinase 8 interacting protein 3 (Mapk8ip3 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pLJC2-Blast-DHFR

Plasmid

#233893

Purpose

lentiviral overexpression vector for DHFR

Depositor

Jason Cantor

Article

Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

Insert

DHFR (DHFR Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Hygro DEST ERKKTRmCerulean3

Plasmid

#90229

Purpose

Lentiviral vector to express ERK KTR mCerulean3 under PGK promoter (With Hygromycin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

ERK Kinase Translocation Reporter

Use

Lentiviral

Tags

mCerulean3

Expression

Mammalian

Promoter

PGK

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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pcdna3 T7 JIP3a

Plasmid

#53457

Purpose

interacts with MAPK signaling module

Depositor

Roger Davis

Article

Kelkar et al Mol Cell Biol. 2000 Feb;20(3):1030-43.

Insert

mitogen-activated protein kinase 8 interacting protein 3 (Mapk8ip3 Mouse)

Tags

T7

Expression

Mammalian

Available Since

Aug. 22, 2014

Availability

Academic Institutions and Nonprofits only
pKrox24(2xD-E)DsRed

Plasmid

#200111

Purpose

Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

modified EGR1 promoter

Expression

Mammalian

Mutation

highly modified sequence

Promoter

2 copies of D-E element, no other promoter elemen…

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-PKI nls

Plasmid

#118301

Purpose

Expression of PKIa

Depositor

Philip Stork

Article

Billiard et al J Biol Chem. 2001 Aug 17;276(33):31238-4

Insert

PKI nls (aa 2-36)

Tags

Flag and GFP

Expression

Mammalian

Mutation

inserting 2xnls

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pBMN-HA-TBK1

Plasmid

#199205

Purpose

Used for the expression of TBK1 (SMC Internal No. 1996)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

TBK1 (TBK1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-p38alpha

Plasmid

#86832

Purpose

mammalian expression of GFP-p38alpha

Depositor

Rony Seger

Article

Zehorai et al Mol Cell Biol. 2014 Jan;34(2):259-70. do

Insert

p38alpha (MAPK14 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 23, 2017

Availability

Academic Institutions and Nonprofits only

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