We narrowed to 17,417 results for: She

1. pET-21a(+)-BMTU UDG-6xHis

   Plasmid

   #172197

   Purpose

   expresses His-tagged uracil-DNA-glycosylase enzyme from marine bacterium BMTU 3346 for the purpose of cross contamination prevention in nucleic acid amplification reactions

   Depositor

   Andrea Pauli

   Insert

   BMTU UDG
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. VPS13D^mScarlet

   Plasmid

   #174110

   Purpose

   The insert is a codon optimized DNA sequence encoding VPS13D with an internal mScarlet protein.

   Depositor

   Pietro De Camilli

   Article

   Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

   Insert

   Codon optimized VPS13D
   

   Use

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   With an internal mScarlet tag in aminoacid positi…

   Promoter

   CMV

   Available since

   Dec. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1 myc TSC1

   Plasmid

   #12133

   Depositor

   Cheryl Walker

   Article

   Cai et al J Cell Biol. 2006 Apr 24. 173(2):279-89.

   Insert

   TSC1 (TSC1 Human)
   

   Use

   Tags

   his and myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pelBHisTEV

   Plasmid

   #157741

   Purpose

   empty vector; TEV-protease-removable N-term PelB signal sequence +His10; potential C-term His6; T7lac promoter; Kan-R; avoids added residues in final protein since cloning uses BseRI restriction sites

   Depositor

   Debra Hansen

   Type

   Empty backbone

   Use

   Tags

   PelB signal sequence + His10-tag + TEV protease c…

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. AICSDP-39:RAB5A-mEGFP

   Plasmid

   #107579

   Purpose

   Homology arms and mEGFP-linker sequence for N-terminus tagging of human RAB5A

   Depositor

   Allen Institute for Cell Science

   Insert

   RAB5A Homology Arms with mEGFP-linker (RAB5A Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   May 9, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. p-dCas9-TET1-Hygro

   Plasmid

   #104404

   Purpose

   transient expression of dCas9-TET1 fusion protein

   Depositor

   Stephan Beck

   Insert

   TET1 (TET1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. MECP2 in pENTR

   Plasmid

   #216592

   Purpose

   gateway cloning donor vector containing MECP2

   Depositor

   Douglas Allan, Christopher Loewen

   Insert

   MECP2 (MECP2 Human)
   

   Use

   Gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TC1541A

   Plasmid

   #164875

   Purpose

   Split GFP reporter expressing GFP(beta1-10) following C>U editing of the target site

   Depositor

   Tian Chi

   Article

   Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:

   Insert

   Split GFP(beta1-10)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTmpKm

   Plasmid

   #176227

   Purpose

   A template plasmid for amplification of the cI-hok-neo cassette

   Depositor

   Sergey Sineoky

   Article

   Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

   Insert

   cI of phage lambda, hok of the R1 plasmid, neo
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-CMV-FLEX-SaCas9-U6-sgHtr2a

    Plasmid

    #159908

    Purpose

    Mutagenesis of Htr2a

    Depositor

    Larry Zweifel

    Insert

    Htr2a (Htr2a Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmTurquoise2-Drp1

    Plasmid

    #110946

    Purpose

    Expresses dynamin-like protein 1 fused to mTurquoise2

    Depositor

    Pablo Rivera-Fuentes

    Insert

    Dynamin-1-like protein (DNM1L Human)
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOET5 CVB3-VLP

    Plasmid

    #218146

    Purpose

    Forms coxsackievirus 3-like particle in insect cells by expression of VP1–VP4 polyprotein (GeneID M33854.1) and 3CD protease (GeneID M33854.1).

    Depositor

    Minna Hankaniemi

    Inserts

    VP1–VP4
    3CD
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    CMV and Polyhedrin

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA3-FLAG-SENP3

    Plasmid

    #115521

    Purpose

    Mammalian expression construct

    Depositor

    Martine Roussel

    Article

    Kuo et al Cell Cycle. 2008 Nov 1;7(21):3378-87. do

    Insert

    SENP3 (Senp3 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lamin A (G465D)-mRFP

    Plasmid

    #124271

    Purpose

    Lamin A mutant (G465D), C-terminally tagged with mRFP. Mutation associated with FPLD lipodystrophy.

    Depositor

    Eric Schirmer

    Insert

    LMNA (G465D) (LMNA Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    LMNA (G465D)

    Promoter

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. DYRK1A

    Plasmid

    #38913

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    DYRK1A (DYRK1A Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    codon-optimized

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pJCX3-ACTG2_R257C

    Plasmid

    #228911

    Purpose

    Expresses mutant R257C smooth muscle actin gamma-2 in human cells

    Depositor

    Roberto Dominguez

    Insert

    Actin gamma 2, smooth muscle (ACTG2 Human)
    

    Use

    Tags

    HIS-FLAG-TEV

    Expression

    Bacterial and Mammalian

    Mutation

    R257C

    Promoter

    CMV

    Available since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJCX3-ACTG2_WT

    Plasmid

    #228910

    Purpose

    Expresses wildtype smooth muscle actin gamma-2 in human cells

    Depositor

    Roberto Dominguez

    Insert

    Actin gamma 2, smooth muscle (ACTG2 Human)
    

    Use

    Tags

    HIS-FLAG-TEV

    Expression

    Bacterial and Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAG416-GAL-FUS-G156E

    Plasmid

    #181716

    Purpose

    Galactose inducible expression of FUS-G156E

    Depositor

    Alejandro Chavez

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    G156E

    Promoter

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGas-miRFP670nano

    Plasmid

    #127441

    Purpose

    G protein alpha-s internally tagged with miRFP670nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

    Insert

    G protein alpha-s-miRFP670nano
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGFP Cas

    Plasmid

    #50729

    Purpose

    mammalian expression of GFP-Cas. Note-This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

    Depositor

    Kenneth Yamada

    Insert

    Cas (Bcar1 Rat)
    

    Use

    Tags

    FLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. OA-1067B

    Plasmid

    #200252

    Purpose

    pBac-U6-gRNA(doublesex)-3xp3-tdTomato

    Depositor

    Omar Akbari

    Article

    Li et al bioRxiv. 2023 Nov 15:2023.04.18.537404.

    Insert

    4 gRNAs targeting Doublesex
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pPB-CAG-Dest-pA-pgk-bsd

    Plasmid

    #74918

    Purpose

    Gateway destination piggyBac vector

    Depositor

    Jose Silva

    Article

    dos Santos et al Cell Stem Cell. 2014 Jul 3;15(1):102-10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 26, 2016

    Availability

    Academic Institutions and Nonprofits only

23. pTmpSm

    Plasmid

    #176228

    Purpose

    A template plasmid for amplification of the cI-hok-strA cassette

    Depositor

    Sergey Sineoky

    Article

    Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

    Insert

    cI of phage lambda, hok of the R1 plasmid, strA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-GFAP-HA-hM3D(Gq)-IRES-mCitrine

    Plasmid

    #50470

    Purpose

    Gq-coupled hM3D-IRES-mCitrine under the control of GFAP promoter

    Depositor

    Bryan Roth

    Insert

    hM3D(Gq)-IRES-mCitrine (CHRM3 Human)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    GFAP

    Available since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Flag FoxO3

    Plasmid

    #14937

    Depositor

    Joan Massague

    Article

    Seoane et al Cell. 2004 Apr 16. 117(2):211-23.

    Insert

    FoxO3 (FOXO3 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. BRAF in pENTR

    Plasmid

    #216605

    Purpose

    gateway cloning donor vector containing BRAF

    Depositor

    Douglas Allan, Christopher Loewen

    Insert

    BRAF (BRAF Human)
    

    Use

    Gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Hs.KRAS4b G12D

    Plasmid

    #83131

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12D mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    G12D

    Promoter

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBad-PhoCl2f

    Plasmid

    #164036

    Purpose

    Improved PhoCl variant with faster dissociation rate

    Depositor

    Robert Campbell

    Article

    Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

    Insert

    PhoCl2f
    

    Use

    Tags

    His tag, T7 tag, Xpress Tag

    Expression

    Bacterial

    Mutation

    Promoter

    araBad promotor

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Hs.KRAS4b

    Plasmid

    #83129

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. DL033:cFos::eGFP-iPAK4

    Plasmid

    #177883

    Purpose

    eGFP-iPAK4 fusion with cFos promoter, cloned into a lentivirus backbone

    Depositor

    Adam Cohen

    Article

    Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    iPAK4
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    cFos

    Available since

    July 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAAV-DIO-CAG-TVA-P2A-dTomato

    Plasmid

    #177016

    Purpose

    For expression of the avian TVA receptor in a cre-dependent manner

    Depositor

    Peter Jonas

    Insert

    TVA-P2A-dTomato (CD320 Coturnix japonica)
    

    Use

    AAV and Cre/Lox

    Tags

    dTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. EEF1A2 in pENTR

    Plasmid

    #216585

    Purpose

    gateway cloning donor vector containing EEF1A2

    Depositor

    Douglas Allan, Christopher Loewen

    Insert

    EEF1A2 (EEF1A2 Human)
    

    Use

    Gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET28a-UBC9

    Plasmid

    #228949

    Purpose

    express a fragment of the wild-type human UBC9 (residues 2-158 of UniProt P63279 supplemented with the N-terminal thrombin-cleavable MGHHHHHHSSGLVPR|G- tag)

    Depositor

    Marcin Suskiewicz

    Insert

    UBC9 (UBE2I Human)
    

    Use

    Tags

    His tag and thrombine cleavage site : MGHHHHHHSSG…

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lac

    Available since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAAV-hSyn-GluA1-scFv-mNeon-KDEL

    Plasmid

    #230056

    Purpose

    AAV plasmid that expresses a single chain variable fragment (scFv) for anti-GluA1 tagged with mNeon. Encodes an ER retrieval motif (KDEL) at C-terminus of mNeon.

    Depositor

    Matthew Kennedy

    Insert

    Anti-GluA1 single chain variable fragment (scFv)
    

    Use

    AAV

    Tags

    ER retrieval motif (KDEL) and mNeon

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. psd44-G12QL

    Plasmid

    #46825

    Purpose

    expression of mutant G protein alpha 12 in mammalian cells

    Depositor

    Agnese Mariotti

    Insert

    GNA12 (GNA12 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Q231L (the mutation reduces GTPase activity resul…

    Promoter

    PGK

    Available since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Hs.KRAS4a

    Plasmid

    #83166

    Purpose

    Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBbS5c-mCherry-AsLOV2*(543)

    Plasmid

    #202712

    Purpose

    Expresses an IPTG inducible mCherry fused to a blue light responsive degradation tag (LOVdeg)

    Depositor

    Mary Dunlop

    Article

    Tague et al Elife. 2024 Jan 25;12:RP87303. doi: 10.7

    Insert

    mCherry-AsLOV2*(543)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. AICSDP-117:POLR2A-mEGFP

    Plasmid

    #164500

    Purpose

    Homology arms and mEGFP-linker sequence sequence for N-terminus tagging of human POLR2A

    Depositor

    Allen Institute for Cell Science

    Insert

    POLR2A Homology Arms with mEGFP-linker (POLR2A Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    Feb. 17, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. pAAV-Ef1a-Flex-Axon-GCaMP7b

    Plasmid

    #135419

    Purpose

    Can be used to drive axon-enriched expression of GCaMP7b in the presence of Cre recombinase.

    Depositor

    Rylan Larsen

    Insert

    axon-jGCaMP7b
    

    Use

    AAV

    Tags

    6xHis and GAP43 palmitoylation domain

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Feb. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET21a-Core Traptavidin

    Plasmid

    #26054

    Depositor

    Mark Howarth

    Article

    Chivers et al Nat Methods. 2010 May;7(5):391-3. doi: 1

    Insert

    Core Traptavidin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Streptavidin with 2 amino acid mutations: S52G R5…

    Promoter

    Available since

    Aug. 30, 2010

    Availability

    Academic Institutions and Nonprofits only