We narrowed to 18,569 results for: MUT

1. pQE30-TEV-pro-conA

   Plasmid

   #159527

   Purpose

   Expresses pro-concanavalin A, a lectin from Canavalia ensiformis, in E. coli. Includes N-terminal TEV recognition site. Native N-terminal sequence is reconstituted upon TEV cleavage.

   Depositor

   Joshua Mylne

   Article

   Nonis et al bioRxiv. 2020.

   Insert

   Canavalia ensiformis pro-concanavalin A
   

   Tags

   Six-Histidine tag and tobacco etch virus (TEV) pr…

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEYFP-N1-HsSmg5_H

   Plasmid

   #146474

   Purpose

   Mammalian Expression of HsSmg5

   Depositor

   Elisa Izaurralde

   Article

   Unterholzner et al Mol Cell. 2004 Nov 19. 16(4):587-96.

   Insert

   HsSmg5 (SMG5 Human)
   

   Expression

   Mammalian

   Mutation

   three non silent mutation A254G (K85R), T980C (L3…

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p23-DDX21 DEV

   Plasmid

   #128805

   Purpose

   overexpression of DDX21 DEV mutation in human cells

   Depositor

   Ling-Ling Chen

   Article

   Xing et al Cell. 2017 May 4;169(4):664-678.e16. doi

   Insert

   DDX21 DEV mutation
   

   Tags

   flag

   Expression

   Mammalian

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPL5628_TEF1*-Cre_MET15

   Plasmid

   #60933

   Purpose

   Constitutive expression vector for Cre-recombinase containing MET15 selection marker.

   Depositor

   Robert Piper

   Article

   MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

   Insert

   Cre
   

   Tags

   Driven from a mutant TEF1 promoter with 0.16x act…

   Expression

   Yeast

   Promoter

   mutant TEF1

   Available Since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. JP1520 PIK3CA E545K HA

   Plasmid

   #14571

   Depositor

   Joan Brugge

   Article

   Isakoff et al Cancer Res. 2005 Dec 1. 65(23):10992-100

   Insert

   PIK3CA E545K (PIK3CA Bovine)
   

   Use

   Retroviral

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   E545K

   Available Since

   April 12, 2007

   Availability

   Academic Institutions and Nonprofits only

6. Venus-Bik-DD-pEGFP-C1

   Plasmid

   #177429

   Purpose

   To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, phosphomimetic mutant. T33D and S35D mutations made to Addgene #166737.

   Depositor

   David Andrews

   Insert

   Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
   

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Threonine 33 and Serine 35 replaced with aspartic…

   Promoter

   CMV

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. R-G324D-(Kin-8)

   Plasmid

   #28179

   Depositor

   Bernard Schimmer

   Article

   Olson et al Mol Endocrinol. 1993 Apr . 7(4):477-87.

   Insert

   Prkar1a (PRKAR1A Mouse)
   

   Expression

   Mammalian

   Mutation

   G324D

   Available Since

   May 12, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3-optoB2-SS-YFP

   Plasmid

   #75270

   Purpose

   Arrestin independent version of optoB2 with serine mutations at S362A,S363G

   Depositor

   Michael Bruchas

   Article

   Siuda et al Nat Commun. 2015 Sep 28;6:8480. doi: 10.

   Insert

   Arrestin independent version of optoB2
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   serine mutations at S362A,S363G

   Available Since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMyc0ElbLuc

   Plasmid

   #53242

   Purpose

   reporter plasmid without myc cDNA binding sites

   Depositor

   Roger Davis

   Article

   Gupta et al Proc Natl Acad Sci U S A. 1993 Apr 15;90

   Insert

   pMycOElbLuc (MYC Human)
   

   Available Since

   July 29, 2014

   Availability

   Academic Institutions and Nonprofits only

10. lentiCRISPRv2-Blast-mU6

    Plasmid

    #206806

    Purpose

    pLentiCRISPRv2 variant with mU6 promoter driving the sgRNA, and a Blasticidin resistance cassette

    Depositor

    Jolanda Van Leeuwen

    Article

    Unlu et al Genome Med. 2023 Oct 12;15(1):78. doi: 1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMB62

    Plasmid

    #47944

    Purpose

    Expresses C. elegans optimized Cas9::mEGFP from the eef-1A.1 (eft-3) promoter

    Depositor

    Mike Boxem

    Article

    Waaijers et al Genetics. 2013 Aug 26.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, egl-13 NLS, and mEGFP

    Expression

    Worm

    Promoter

    eef-1A.1 (eft-3)

    Available Since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDREV-2

    Plasmid

    #26752

    Depositor

    Rolf Zeller

    Article

    Osterwalder et al Nat Methods. 2010 Oct 17

    Insert

    H2B-Venus CDS (H2BC3 Human)
    

    Use

    Cre/Lox; Flp/frt; dre/rox

    Tags

    Venus

    Expression

    Mammalian

    Available Since

    Jan. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBA1032

    Plasmid

    #189580

    Purpose

    T4soc deletion HR vector 250bp HR Arms (T4 phage editing)

    Depositor

    Jennifer Doudna

    Article

    Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

    Insert

    T4soc Deletion Locus 250bp Homology Arms
    

    Use

    Unspecified

    Mutation

    Encodes for a full deletion of T4soc

    Available Since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDEST-HemmarI2

    Plasmid

    #112815

    Purpose

    gateway cloning destination vector for expressing enhancer driven CD4-IFP2.0-T2A-HO1

    Depositor

    Chun Han

    Article

    Poe et al Genetics. 2018 Nov 30. pii: genetics.118

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCEP4-DAT-myc

    Plasmid

    #107457

    Purpose

    Mammalian expression plasmid for codon optimized hDAT with internal MYC tag inserted in ECL2

    Depositor

    Erik Procko

    Article

    Young et al bioRxiv 2021.04.19.440442

    Insert

    Dopamine Transporter (DAT) (SLC6A3 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mCh-LaGFP(N)-cpFRB2-T2A-FKBP-LaGFP(C)

    Plasmid

    #165030

    Purpose

    Express cpFRB2-based GFP specific Chessbody (chemically switchable split nanobody); visualized by mCherry

    Depositor

    Yubin Zhou

    Article

    Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

    Insert

    LaGFP(N)-cpFRB2-T2A-FKBP-LaGFP(C)
    

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Promoter

    T7

    Available Since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAMP-CY1_NF1 T2 (+minintr, KDR)

    Plasmid

    #119201

    Purpose

    transfer vector

    Depositor

    Helen Morrison

    Article

    Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

    Insert

    NF1 type 2 mini-gene (NF1 Human)
    

    Use

    Transfer vector

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCXN2-mVIN

    Plasmid

    #11776

    Depositor

    Eileen Adamson

    Article

    Xu et al J Cell Sci. 1998 Jun;111 ( Pt 11):1535-4

    Insert

    vinculin (Vcl Mouse)
    

    Expression

    Mammalian

    Available Since

    Oct. 20, 2006

    Availability

    Academic Institutions and Nonprofits only

19. Lamin A (R527P)-mRFP

    Plasmid

    #124272

    Purpose

    Lamin A mutant (R527P), C-terminally tagged with mRFP. Mutation associated with EDMD myopathy.

    Depositor

    Eric Schirmer

    Insert

    LMNA (R527P) (LMNA Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    LMNA (R527P)

    Available Since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pV1435

    Plasmid

    #111439

    Purpose

    Solo vector pV1382 + sgCgADE2

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgCgADE2
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge