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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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162,050 results

Showing: 10801 - 10850 of 162050 results

  6. pSBtet-RP

    Plasmid
    #60497
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of RFP, rtTA and puromycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TCE & RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60497-plasmid-map-sequence-id-97550

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  12. pEG302-SunTag-FWAgRNA-4-22aa-TET1cd

    Plasmid
    #115344
    Purpose
    SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoter
    Depositor
    Steven Jacobsen
    Insert
    gRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    March 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    115344-plasmid-map-sequence-id-222905

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  14. pCFD5_w

    Plasmid
    #112645
    Purpose
    Ubiquitous expression of one or multiple sgRNAs in Drosophila. Selection marker: Mini-white.
    Depositor
    Michael Boutros
    Insert
    U6:3-tRNA-sgRNA expression cassette
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    112645-plasmid-map-sequence-id-217990

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  15. HA-CHMP4B

    Plasmid
    #139013
    Purpose
    Expresses HA-tagged human CHMP4B in mammalian cells
    Depositor
    Ken-Ichi Takemaru
    Insert
    human CHMP4B
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    139013-plasmid-map-sequence-id-274403

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  32. pLenti-CMV-CAG-GFP-EnvA(N2c)

    Plasmid
    #194353
    Purpose
    Lentivirus expressing eGFP and (EnvA-N2c Rabies Glycoprotein) fusion protein. Used to package EnvA pseudotyped G-deleted Rabies of CVS-N2c strain
    Depositor
    Marco Tripodi
    Article
    Lee et al Nat Methods. 2023 Apr;20(4):580-589. doi
    Inserts
    Enhanced Green Fluorescent Protein (eGFP)
    EnvA-CVS-N2c Rabies Glycoprotein
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    194353-plasmid-map-sequence-id-391646

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  33. B-GRAM-W

    Plasmid
    #211707
    Purpose
    Expression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187W mutation (B-GRAM-W) in mammalian cells
    Depositor
    Yasunori Saheki
    Article
    Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:
    Insert
    B-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    Use
    Tags
    ddFP-B3
    Expression
    Mammalian
    Mutation
    Changed Glycine 187 to Tryptophan
    Promoter
    CMV
    Available since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    211707-plasmid-map-sequence-id-419219

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  35. pCasso

    Plasmid
    #207530
    Purpose
    Conditionally-replicating in Pseudomonas plasmid for cytidine base editing based on pS44i8GH-2; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b→msfGFP; Pm→CBE:SpRY, PEM7→non-specific sg
    Depositor
    Pablo Ivan Nikel
    Article
    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.
    Insert
    cytidine base editing; oriV(pRO1600/ColE1), xylS, Pm→repA, P14b→msfGFP; Pm→CBE:SpRY, PEM7→non-specific sgRNA
    Use
    CRISPR; Pseudomonas vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    207530-plasmid-map-sequence-id-422503

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  37. pRS418

    Plasmid
    #11256
    Depositor
    Fred Cross
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 15, 2006
    Availability
    Academic Institutions and Nonprofits only
    11256-plasmid-map-sequence-id-6930

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  38. pPROEX-TwStrp-FTO

    Plasmid
    #190265
    Purpose
    Expresses FTO in bacterial cells, with two purification tags: His and Twin-Streptag.
    Depositor
    Samie Jaffrey
    Insert
    FTO (FTO Human)
    Use
    Tags
    6xHis and Twin-Streptag
    Expression
    Bacterial
    Mutation
    Promoter
    Trc
    Available since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    190265-plasmid-map-sequence-id-371437

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  39. pAAV.CAG.Flex.NES-jRCaMP1b.WPRE.SV40 (AAV1)

    Viral Prep
    #100849-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.CAG.Flex.NES-jRCaMP1b.WPRE.SV40 (#100849). In addition to the viral particles, you will also receive purified pAAV.CAG.Flex.NES-jRCaMP1b.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent NES-jRCaMP1b calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:
    Promoter
    CAG
    Tags
    None
    Available since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only

  40. pEG302 22aa SunTag NtDRMcd (no NLS) g4+g10+g18 (FWA)

    Plasmid
    #117168
    Purpose
    CRISPR Cas9 SunTag system to target NtDRMcd (without an NLS) to the FWA locus with three guide RNAs
    Depositor
    Steven Jacobsen
    Article
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    g18_U6_g10_U6_g4_U6_NOS_NLS_GB1_noNLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    March 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    117168-plasmid-map-sequence-id-238529

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  41. AAV-CAG-jGCaMP8f-WPRE

    Plasmid
    #179254
    Purpose
    AAV-mediated expression of GCaMP8f under the CAG promoter
    Depositor
    Loren Looger
    Has Service
    AAV Retrograde, AAV1, and AAV9
    Insert
    jGCaMP8f
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Feb. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    179254-plasmid-map-sequence-id-381183

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  42. TFORF0799

    Plasmid
    #141705
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ATF3 (ATF3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141705-plasmid-map-sequence-id-404521

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Showing: 10801 - 10850 of 162050 results