165,803 results

1. MAC_N_AURKB

   Plasmid

   #111682

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   AURKB (AURKB Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. NylCK-TS_pET28a

   Plasmid

   #215423

   Purpose

   Expression construct for NylCK-TS gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   NylCK-TS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   S111G/A137L

   Promoter

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti4/V5-DEST-P85

   Plasmid

   #40219

   Depositor

   Lynda Chin

   Insert

   P85 alpha (PIK3R1 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 11, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pCW-Tet-Cas9-BFP-PGK-Blast

   Plasmid

   #196720

   Purpose

   Tet-inducible expression of Cas9-T2A-TagBFP

   Depositor

   Bernhard Schmierer

   Insert

   Cas9-T2A-TagBFP
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG-SV40NLS and Nucleoplasmin NLS

   Expression

   Mutation

   Promoter

   TRE, PGK

   Available Since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGBW-m4134908

   Plasmid

   #151957

   Purpose

   Mammalian expression plasmid for Human coronavirus NL63 nucleocapsid

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   Human coronavirus NL63 nucleocapsid protein (HCNV63gp6 Human coronavirus NL63)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   CMV

   Available Since

   May 20, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pLXSN p110 CUX1

   Plasmid

   #90471

   Purpose

   Retroviral vector expressing human p110 CUX1 (amino acids 747-1505) with a Myc and HA tag at the N- and C-terminue, respectively

   Depositor

   Alain Nepveu

   Article

   Kedinger et al J Biol Chem. 2009 Oct 2;284(40):27701-11

   Insert

   CUX1 (amino acids 747-1505) (CUX1 Human)
   

   Use

   Retroviral

   Tags

   HA and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Moloney murine leukemia virus long terminal repeat

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMGF196

   Plasmid

   #97005

   Purpose

   Expresses LEMD2-mCherry in mammalian cells under a crippled promoter

   Depositor

   Wesley Sundquist, Adam Frost

   Article

   Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

   Insert

   LEMD2 (LEMD2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV delta 5

   Available Since

   Aug. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-hRARα.hRXRα

   Plasmid

   #135411

   Purpose

   Human Retinoic Acid Receptor-alpha and Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector

   Depositor

   Catharine Ross

   Article

   Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

   Inserts

   Human retinoic acid receptor-alpha
   Human retinoid X receptor-alpha
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and SV40

   Available Since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEGFP-C1-Ataxin3Q84

   Plasmid

   #22123

   Depositor

   Henry Paulson

   Article

   Chai et al Proc Natl Acad Sci U S A. 2002 Jul 9. 99

   Insert

   Ataxin 3 (ATXN3 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 2, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFETCh_Donor (EMM0021)

    Plasmid

    #63934

    Purpose

    3X Flag with P2A-Neo for 3' tagging of endogenous proteins

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    Glycine-Serine-3XFlag-P2A-NeoR

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLXSN16E6E7

    Plasmid

    #52394

    Purpose

    The plasmid, pLXSN16E6E7, is used to produce amphotropic recombinant retrovirus providing efficient gene transfers and expression of the HPV16 E6 and E7 genes.

    Depositor

    Denise Galloway

    Article

    Halbert et al J Virol. 1991 Jan;65(1):473-8.

    Insert

    HPV16 E6E7
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLXSN p200 CUX1

    Plasmid

    #90470

    Purpose

    Retroviral vector expressing human p200 CUX1 with a Myc and HA tag at the N- and C-terminus, respectively

    Depositor

    Alain Nepveu

    Article

    Kedinger et al J Biol Chem. 2009 Oct 2;284(40):27701-11

    Insert

    CUX1 (CUX1 Human)
    

    Use

    Retroviral

    Tags

    Ha and myc

    Expression

    Mammalian

    Mutation

    Promoter

    Moloney murine leukemia virus long terminal repeat

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. JB85

    Plasmid

    #128909

    Purpose

    mammalian expression, proximity labelling, mitochondrial relocalisation

    Depositor

    Sean Munro

    Article

    Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

    Insert

    Rab9ASN-BirA*-HA-MAO (RAB9A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pQIq_MRGS_HIS8_(27F3)_FLAG

    Plasmid

    #199615

    Purpose

    Encodes DARPin-FLAG 27F3 for bacterial expression and column purification

    Depositor

    Shiva Tyagarajan

    Article

    Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

    Insert

    DARPin-FLAG 27F3
    

    Use

    Tags

    8xHis and FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available Since

    May 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDGE334

    Plasmid

    #153243

    Purpose

    M2E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Flag-HA-USP8

    Plasmid

    #22608

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP8 (USP8 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available Since

    Dec. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1 TEV (full-length)

    Plasmid

    #64276

    Purpose

    Expresses tobacco etch virus protease (TEV)

    Depositor

    Xiaokun Shu

    Article

    To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

    Insert

    Tobacco etch virus (TEV) protease
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (AAV2)

    Viral Prep

    #105545-AAV2

    Purpose

    Ready-to-use AAV2 particles produced from pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (#105545). In addition to the viral particles, you will also receive purified pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 plasmid DNA. CMV-driven EGFP-Cre expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    HI

    Tags

    EGFP

    Available Since

    Oct. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

19. pMOS003: GCaMPer calcium sensor (endoplasmic reticulum)

    Plasmid

    #163047

    Purpose

    GCaMPer calcium sensor (endoplasmic reticulum)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    GCaMPer
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT7-EGFP-C1-HsPAN2

    Plasmid

    #37373

    Depositor

    Elisa Izaurralde

    Article

    Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

    Insert

    HsPAN2 (PAN2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. ArcLight-A242

    Plasmid

    #36857

    Depositor

    Vincent Pieribone

    Article

    Jin et al Neuron. 2012 Sep 6;75(5):779-85.

    Insert

    fluorescent protein voltage sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Ci-VSP contains R217Q mutation; super ecliptic pH…

    Promoter

    semian CMV

    Available Since

    Aug. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRK5_mEGFP-NUP98-HOXA9

    Plasmid

    #237639

    Purpose

    For overexpression of mEGFP-NUP98-HOXA9

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    NUP98-HOXA9 (NUP98, HOXA9 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. TRPML1-G-GECO1.2-ERES

    Plasmid

    #207144

    Purpose

    Fusion of Ca2+ reporter and lysosomal Ca2+-permeable channel, with ER-export signal

    Depositor

    Antony Galione

    Article

    Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

    Insert

    Mcoln1 (MCOLN1 Human)
    

    Use

    Tags

    ER-Export Signal (ERES) and G-GECO1.2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV-dCas13-M3nls

    Plasmid

    #155366

    Purpose

    Targeted m6A RNA methylation in mammalian cells

    Depositor

    David Liu

    Article

    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

    Insert

    dCas13-M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    PspCas13b Δ984-1090 H133A

    Promoter

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMU-TGNVHAr

    Plasmid

    #61178

    Purpose

    Fluorescent reporter for trans-Golgi network AtVHA-a1-mCherry expressed under AtUBQ10 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    AtVHA-a1
    

    Use

    Tags

    mCherry

    Expression

    Plant

    Mutation

    Promoter

    AtUBQ10

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-CAG-DIO-mTurquoise2

    Plasmid

    #104059

    Purpose

    AAV expression of Cre-dependent reporter mTurquoise2

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    mTurquoise2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSin-EF2-Sox2-Pur

    Plasmid

    #16577

    Depositor

    James Thomson

    Article

    Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

    Insert

    Homo sapiens SRY (sex determining region Y)-box 2 (SOX2) (SOX2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. mCherry-LC-Myosin-N-7

    Plasmid

    #55072

    Purpose

    Localization: Light Chain Myosin, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    LC-Myosin
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

29. hCRISPRi-v2, Membrane Proteins (h6), top 5 sgRNAs/gene

    Pooled Library

    #83976

    Purpose

    Human CRISPRi Pooled Library targeting membrane proteins

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

    Available Since

    Dec. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

30. huCaV2.2 (pSAD442-1)

    Plasmid

    #62574

    Purpose

    Human CaV2.2 expression in mammalian cells

    Depositor

    Diane Lipscombe

    Article

    Groen et al Hum Mol Genet. 2015 Feb 15;24(4):987-993

    Insert

    CACNA1B (CACNA1B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3-Akt-STOPS

    Plasmid

    #175006

    Purpose

    Akt Substrate-based Tandem Occupancy Peptide Sponge. Genetically encoded for perturbing cellular Akt kinase activity.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    mCherry-Akt-STOPS
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pHybrid-Electra2

    Plasmid

    #179475

    Purpose

    Expresses Electra2 in bacterial cells and Electra2 and mScarlet in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Inserts

    Electra2
    mScarlet-I
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMpGWB104

    Plasmid

    #68558

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    GUS

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCMV-AsCas12f1

    Plasmid

    #171614

    Purpose

    AsCas12f1-based Human cell genome editing

    Depositor

    Quanjiang Ji

    Article

    Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

    Insert

    AsCas12f1 and sgRNA_v1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCDNA3-hTICAM1-VenusFlag

    Plasmid

    #197632

    Purpose

    Human TRIF protein expression in mammalian cells

    Depositor

    Haian Fu

    Insert

    TICAM1 (TICAM1 Human)
    

    Use

    Tags

    Venus-Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEJS2423_Nme2.Smu-ABE8e-i7

    Plasmid

    #201521

    Purpose

    Mammalian expression of domain inlaid Nme2.Smu-ABE8e-i7

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

    Insert

    Nme2.Smu-ABE8e-i7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. mCardinal-ZO1-C-14

    Plasmid

    #56179

    Purpose

    Localization: Tight Junctions, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    ZO1 (TJP1 Human)
    

    Use

    Tags

    mCardinal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 4, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pCX-2A-2A

    Plasmid

    #74670

    Purpose

    based on pCX-C1 with F2A1/F2A2 inserted

    Depositor

    Roberto Giovannoni

    Article

    De Giorgi et al Plasmid. 2015 May;79:22-9. doi: 10.1016/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCAGGS

    Available Since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. HP4_M13

    Plasmid

    #120340

    Purpose

    Helper plasmid for production of phagemid ssDNA, based on m13mp18

    Depositor

    Hendrik Dietz

    Article

    Praetorius et al Nature. 2017 Dec 6;552(7683):84-87. doi:

    Insert

    Coding part of the M13 phage genome
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    native phage promoters

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCDNA5/FRT_CMV_NES-Caprola_04-mEGFP

    Plasmid

    #194682

    Purpose

    CMV driven expression of the calcium recorder Caprola_04 in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_04-mEGFP
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge