Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

161,255 results

Showing: 8681 - 8720 of 161255 results

  1. pAAV-CaMKIIa-hM3D(Gq)-mCherry (AAV2)

    Viral Prep
    #50476-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV-CaMKIIa-hM3D(Gq)-mCherry (#50476). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hM3D(Gq)-mCherry plasmid DNA. CaMKIIa-driven hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    CaMKII
    Tags
    mCherry
    Available since
    Jan. 26, 2021
    Availability
    Academic Institutions and Nonprofits only

  2. mAIRN HO

    Plasmid
    #207411
    Purpose
    Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).
    Depositor
    Karlene Cimprich
    Article
    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do
    Insert
    mAIRN
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    207411-plasmid-map-sequence-id-422696

    Enlarge

  3. mAIRN CD

    Plasmid
    #207412
    Purpose
    Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).
    Depositor
    Karlene Cimprich
    Article
    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do
    Insert
    mAIRN
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    207412-plasmid-map-sequence-id-422689

    Enlarge

  8. pGP-AAV-syn-jGCaMP7s-WPRE (AAV Retrograde)

    Viral Prep
    #104487-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pGP-AAV-syn-jGCaMP7s-WPRE (#104487). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP7s-WPRE plasmid DNA. Synapsin-driven GCaMP7s calcium sensor. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project, Douglas Kim
    Article
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Promoter
    Syn
    Tags
    None
    Available since
    May 31, 2018
    Availability
    Academic Institutions and Nonprofits only

  9. mKate2-P2A-APEX2-GBP

    Plasmid
    #67650
    Purpose
    Mammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody). Also contains bicistronially expressed cytoplasmic red fluorescent reporter.
    Depositor
    Rob Parton
    Article
    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:
    Insert
    APEX2-GBP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    67650-plasmid-map-sequence-id-116990

    Enlarge

  10. pmCherry Vinculin

    Plasmid
    #50527
    Depositor
    Kenneth Yamada
    Insert
    Vinculin (VCL Chicken)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    50527-plasmid-map-sequence-id-137772

    Enlarge

  15. pRDB_347

    Plasmid
    #231114
    Purpose
    CBE activity-based selection cloning vector
    Depositor
    David Root, John Doench
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    231114-plasmid-map-sequence-id-458384

    Enlarge

  17. dTAG-IRF8

    Plasmid
    #190620
    Purpose
    Lentiviral expression plasmid of human IRF8 fused with FKBP12G36V for dTAG-mediated degradation
    Depositor
    Junwei Shi
    Article
    Cao et al Mol Cell. 2021 Sep 2;81(17):3604-3622.e1
    Insert
    IRF8 (IRF8 Human)
    Use
    Lentiviral
    Tags
    FKBP12_G36V
    Expression
    Mammalian
    Mutation
    synonymous mutation for sgRNA resistance for sgIR…
    Promoter
    Available since
    Oct. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    190620-plasmid-map-sequence-id-372992

    Enlarge

  19. pZP06

    Plasmid
    #232320
    Purpose
    pZP05 was constructed by cloning the oriFn-Pfdx-E-repA fragment from pCWU6 into the smaller suicide plasmid pCM-galK, creating a conditional plasmid with replication controlled by Pfdx-E.
    Depositor
    Chenggang Wu
    Insert
    Pfdx-E
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232320-plasmid-map-sequence-id-462279

    Enlarge

  22. Fragment1 with TLS2

    Plasmid
    #196983
    Purpose
    Gateway-compatible gRNA backbone with TLS2 mobility signal. Template for the addition of target sequences to produce 2x graft-mobile gRNA-TLS2.
    Depositor
    Friedrich Kragler
    Article
    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    Intermediate fragment for assembly of gRNA-TLS2 construct
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196983-plasmid-map-sequence-id-390457

    Enlarge

  23. pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] (AAV Retrograde)

    Viral Prep
    #84445-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] (#84445). In addition to the viral particles, you will also receive purified pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] plasmid DNA. CAG-drive, Cre-dependent Jaws-GFP for optogenetic inhibition. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Article
    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi
    Promoter
    CAG
    Tags
    GFP (Cre-dependent)
    Available since
    Oct. 12, 2017
    Availability
    Academic Institutions and Nonprofits only

  30. pDEST-CMV-polysite

    Plasmid
    #122843
    Purpose
    Gateway destination vector for mammalian expression without tag, or for adding custom tags by conventional cloning. Distinct multiple cloning sites 5' and 3' of gateway cassette.
    Depositor
    Robin Ketteler
    Article
    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155
    Type
    Empty backbone
    Use
    Gateway destination
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    122843-plasmid-map-sequence-id-244374

    Enlarge

  35. pCEP4-M2L

    Plasmid
    #20926
    Purpose
    used in the derivation of human iPS cells using non-integrating episomal vectors; expresses cMyc and Lin28
    Depositor
    James Thomson
    Article
    Yu et al Science. 2009 Mar 26.
    Inserts
    MYC (MYC Human)
    LIN28 (LIN28A Human)
    Use
    Episomal/ebna
    Tags
    Expression
    Mutation
    Promoter
    CMV and IRES
    Available since
    May 20, 2009
    Availability
    Academic Institutions and Nonprofits only
    20926-plasmid-map-sequence-id-9762

    Enlarge

  36. pAAV-hSyn-dLight1.2 (AAV1)

    Viral Prep
    #111068-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-hSyn-dLight1.2 (#111068). In addition to the viral particles, you will also receive purified pAAV-hSyn-dLight1.2 plasmid DNA. Synapsin-driven expression of dLight1.2 dopamine sensor. This sensor has a slightly increased dynamic range and is better for 2P imaging in vivo. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Lin Tian
    Article
    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci
    Promoter
    Tags
    None
    Available since
    Feb. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 8681 - 8720 of 161255 results