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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 25,508 results for: crispr

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Showing: 4541 - 4560 of 25508 results

  1. KRAB-dCas9

    Plasmid
    #112195
    Purpose
    KRAB fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Publication
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KRAB (ZNF10 Human)
    Use
    CRISPR
    Tags
    3XFLag-NLS-KRAB-dCas9-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    112195-plasmid-map-sequence-id-213538

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  3. pL-SFFV.Reporter.RFP657.PAC

    Plasmid
    #61395
    Purpose
    Lentiviral vector for RFP657 NHEJ reporter system expression. Allows insertion of target sites between the SFFV promoter and RFP657 for detection of fluorescence knockdown. Puromycin resistance.
    Depositor
    Benjamin Ebert
    Publication
    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    SFFV
    Available since
    May 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61395-plasmid-map-sequence-id-101662

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  4. pDT305

    Plasmid
    #128801
    Purpose
    Human codon optimized dCas9-Dnmt3a-Dnmt3l-T2A-eGFP + gRNA expression cassette + LV backbone
    Depositor
    Bradley Bernstein
    Publication
    Tarjan et al Nat Commun. 2019 Sep 18;10(1):4258. doi:
    Insert
    dCas9-Dnmt3a-Dnmt3l-T2A-eGFP + gRNA expression cassette + LV backbone
    Use
    CRISPR and Lentiviral
    Promoter
    hUBC promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    128801-plasmid-map-sequence-id-255180

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  9. pKMD111e - intein-Cas9(S219-G521R)

    Plasmid
    #64192
    Purpose
    Expresses intein-Cas9(S219-G521R) in mammalian cells
    Depositor
    David Liu
    Publication
    Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized S. pyogenes intein-Cas9(S219-G521R) - NLS - 3x FLAG
    Use
    CRISPR
    Tags
    NLS - 3x FLAG (C terminal on insert)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    64192-plasmid-map-sequence-id-107551

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  10. pSmart-Nm-sgRNA-BbsI

    Plasmid
    #49157
    Purpose
    Plasmid for cloning spacer into sgRNA for NmCas9
    Depositor
    James Thomson
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Feb. 13, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    49157-plasmid-map-sequence-id-72446

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  13. Sc++

    Plasmid
    #155011
    Purpose
    Expresses the codon-optimized, engineered Sc++ Cas9 nuclease in mammalian cells
    Depositor
    Joseph Jacobson
    Publication
    Chatterjee et al Nat Biotechnol. 2020 May 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Sc++
    Expression
    Mammalian
    Mutation
    T1227K, Loop Substitution from S. anginosus
    Promoter
    EF1ɑ
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    155011-plasmid-map-sequence-id-308829

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  15. pMH3

    Plasmid
    #52528
    Purpose
    serves as PCR-template to generate HR donors for C-terminal GFP-tag
    Depositor
    Klaus Foerstemann
    Publication
    Bottcher et al Nucleic Acids Res. 2014 Apr 19.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eGFP and Blasticigin resistance cassette
    Use
    Blunt cloning kit
    Available since
    April 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    52528-plasmid-map-sequence-id-81488

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Showing: 4541 - 4560 of 25508 results