We narrowed to 23,648 results for: crispr

1. pCF525-mTagBFP2_EF1a-Hygro-P2A-mTagBFP2_lenti

   Plasmid

   #138315

   Purpose

   Lenti expression of mTgBFP2

   Depositor

   Jennifer Doudna

   Article

   Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

   Insert

   mTAGBFP2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   March 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ142

   Plasmid

   #69294

   Purpose

   Golden Gate recipient and Gateway entry vector; assembly of 2 gRNAs

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Nov. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPQ144

   Plasmid

   #69296

   Purpose

   Golden Gate recipient and Gateway entry vector; assembly of 4 gRNAs

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. LentiGuide-TagRFP-2A-BSD

   Plasmid

   #167930

   Purpose

   Lentiviral gRNA expression with TagRFP fluorescence and blasticidin selection marker.

   Depositor

   Ronald Germain

   Article

   Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6, EF1a

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCas9-polyA

   Plasmid

   #72602

   Purpose

   express hCas9 byT7 promoter with polyadenine tails

   Depositor

   Tomoji Mashimo

   Article

   Yoshimi et al Nat Commun. 2016 Jan 20;7:10431. doi: 10

   Insert

   Cas9-polyadenine
   

   Tags

   81-bp polyadenylation

   Promoter

   T7

   Available Since

   July 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. Anep8_Cas9

   Plasmid

   #117169

   Purpose

   Expressed Cas9 in Aspergillus with LIC tags for easy gRNA cloning

   Depositor

   Adrian Tsang

   Article

   Song et al PLoS One. 2018 Aug 24;13(8):e0202868. do

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology; Filamentous fungi e…

   Promoter

   Pki

   Available Since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPEgRNA

   Plasmid

   #172716

   Purpose

   Delivery of guide RNA for prime editing

   Depositor

   Tilmann Weber

   Article

   Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

   Insert

   gRNA
   

   Expression

   Bacterial

   Available Since

   Sept. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLSC-5

   Plasmid

   #62889

   Purpose

   Lenti vector for expression of inducible split Cas9

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

   Inserts

   SpCas9(574-1368)
   SpCas9(2-573)
   

   Use

   CRISPR and Lentiviral

   Tags

   FFBP12, FRB, NES PTK2, NLS SV40, and P2A

   Expression

   Mammalian

   Mutation

   *see comment below

   Promoter

   EFS

   Available Since

   March 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHFS:SEP-rGluA2 TKIT donor

   Plasmid

   #169440

   Purpose

   Donor DNA for TKIT knockin of SEP to GluA2 in rat

   Depositor

   Richard Huganir

   Article

   Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

   Insert

   Super ecliptic pHluorin (SEP)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRU41

    Plasmid

    #167663

    Purpose

    ENTRY vector for gRNA1 cloning under pAtU6 promoter

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pAtU6
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRU42

    Plasmid

    #167664

    Purpose

    ENTRY vector for gRNA2 cloning under pAtU3 promoter

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pAtU3
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRU43

    Plasmid

    #167665

    Purpose

    ENTRY vector for gRNA3 cloning under pAtU6 promoter

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pAtU6
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. vHB8

    Plasmid

    #193454

    Purpose

    Expression of GFP-Cas9-NLS and gRNA in A. castellanii

    Depositor

    Chantal Abergel

    Article

    Bisio et al Nat Commun. 2023 Jan 26;14(1):428. doi:

    Insert

    GFP-Cas9-NLS
    

    Use

    CRISPR

    Tags

    GFP

    Promoter

    GAPDH

    Available Since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCas9

    Plasmid

    #167547

    Purpose

    Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.

    Depositor

    Howard Hang

    Article

    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00

    Inserts

    chloramphenicol acetyl transferase
    pUC19 origin of replication
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-SEP-GluA2 TKIT

    Plasmid

    #169442

    Purpose

    Expression of 2 guides + donor DNA

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    Super ecliptic pHluorin (SEP)
    

    Use

    AAV and CRISPR

    Available Since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. PX856

    Plasmid

    #62888

    Purpose

    C-term SpCas9 piece of inducible transcriptional activator (dCas9(C)-FKBP-2xNLS-VP64)

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa536-1368)
    

    Use

    CRISPR

    Tags

    FKBP12, NLS, NLS SV40, and VP64

    Expression

    Mammalian

    Mutation

    Asparagine 863 to Alanine (N863A)

    Promoter

    CBh

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBA560

    Plasmid

    #186236

    Purpose

    pTet-eLbuCas13a crRNA entry vector

    Depositor

    Jennifer Doudna

    Article

    Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. PX855

    Plasmid

    #62887

    Purpose

    N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa 2-535)
    

    Use

    CRISPR

    Tags

    FRB and NES PTK2

    Expression

    Mammalian

    Mutation

    Aspartic acide 10 to Alanine (D10A)

    Promoter

    CBh

    Available Since

    March 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMiniT:SEP-GluA2 TKIT donor

    Plasmid

    #169421

    Purpose

    Donor DNA for TKIT knockin of SEP to GluA2 in mouse

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    Super ecliptic pHluorin (SEP)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pnsgRNA

    Plasmid

    #172717

    Purpose

    Delivery of guide RNA for prime editing

    Depositor

    Tilmann Weber

    Article

    Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

    Insert

    J23119-gRNA
    

    Expression

    Bacterial

    Available Since

    Sept. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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