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Plasmid#117003PurposeExpresses EsRT-Cas1-Cas2 under pT7lac promoter, encodes EsCRISPR array 2, compatible with SENECA acquisition readoutDepositorInsertEubacterium saburreum RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS_0390_pET30-RT-CRISPR_Eubacterium_saburreum_DSM_3986_ARRAY_1_RC
Plasmid#117002PurposeExpresses EsRT-Cas1-Cas2 under pT7lac promoter, encodes EsCRISPR array 1 RC, compatible with SENECA acquisition readoutDepositorInsertEubacterium saburreum RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS_0389_pET30-RT-CRISPR_Eubacterium_saburreum_DSM_3986_ARRAY_1
Plasmid#117001PurposeExpresses EsRT-Cas1-Cas2 under pT7lac promoter, encodes EsCRISPR array 1, compatible with SENECA acquisition readoutDepositorInsertEubacterium saburreum RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS_0374_pET30-RT-CRISPR_Teredinibacter_turnerae_T8412_ARRAY_1_RC
Plasmid#116986PurposeExpresses TtRT-Cas1-Cas2 under pT7lac promoter, encodes TtCRISPR array 1 RC, compatible with SENECA acquisition readoutDepositorInsertTeredinibacter turnerae T8412 RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS_0372_pET30-RT-CRISPR_Azospirillum_lipoferum_4B_ARRAY_2_RC
Plasmid#116984PurposeExpresses AlRT-Cas1-Cas2 under pT7lac promoter, encodes AlCRISPR array 2 RC, compatible with SENECA acquisition readoutDepositorInsertAzospirillum lipoferum 4B RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS_0373_pET30-RT-CRISPR_Teredinibacter_turnerae_T8412_ARRAY_1
Plasmid#116985PurposeExpresses TtRT-Cas1-Cas2 under pT7lac promoter, encodes TtCRISPR array 1, compatible with SENECA acquisition readoutDepositorInsertTeredinibacter turnerae T8412 RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS_0370_pET30-RT-CRISPR_Azospirillum_lipoferum_4B_ARRAY_1_RC
Plasmid#116982PurposeExpresses AlRT-Cas1-Cas2 under pT7lac promoter, encodes AlCRISPR array 1 RC, compatible with SENECA acquisition readoutDepositorInsertAzospirillum lipoferum 4B RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS_0371_pET30-RT-CRISPR_Azospirillum_lipoferum_4B_ARRAY_2
Plasmid#116983PurposeExpresses AlRT-Cas1-Cas2 under pT7lac promoter, encodes AlCRISPR array 2, compatible with SENECA acquisition readoutDepositorInsertAzospirillum lipoferum 4B RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR sgAMDHD1
Plasmid#102316Purposegenetic depletion of AMDHD1DepositorInsertsgAMDHD1 (AMDHD1 Human)
UseLentiviralTagsExpressionMutationPromoterU6Available sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLENTICRISPR sgNFS1
Plasmid#102979PurposeCutting NFS1 genomic locusDepositorInsertsgNFS1 (NFS1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR-sgC17orf89-2
Plasmid#86135PurposeLentiviral vector expressing Cas9 and an sgRNA targeting C17orf89DepositorInsertsgRNA targeting C17orf89 (NDUFAF8 )
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR-sgC17orf89-3
Plasmid#86136PurposeLentiviral vector expressing Cas9 and an sgRNA targeting C17orf89DepositorInsertsgRNA 3 targeting C17orf89 (NDUFAF8 )
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR-sgC17orf89-1
Plasmid#86137PurposeLentiviral vector expressing Cas9 and an sgRNA targeting C17orf89DepositorInsertsgRNA 1 targeting C17orf89 (NDUFAF8 )
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
TU#1805_CRISPR_unc-73_exon2
Plasmid#82359Purposeto create unc-73B null alleleDepositorInsertCas9 and sgRNA against unc-73 exon2 (unc-73 Nematode)
UseCRISPRTagsExpressionWormMutationPromoterU6Available sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-PsppA-mCherry
Plasmid#225428PurposePlasmid encodes SgRNA, SpCas9 and homologous arms for homologous recombination of mCherryDepositorInsertmCherry, Cas9, SgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterPsppA upstream of mCherry and Cas9, P3 upstream o…Available sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CRISPR-Puro
Plasmid#208646PurposepcDNA3.1 based empty vector for transient transfection of sgRNA-cas9. U6 promoter ~ puro Res cloned from lentiCRISPRv2 (Addgene#52961), lenti virus sequences not included.DepositorTypeEmpty backboneUseCRISPRTagsClawed frog nucleoplasmin NLS, Flag, Puromycin re…ExpressionMammalianMutationPromoterCMV and U6 in tandemAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-PDi-CRISPRn
Plasmid#73500PurposeDox-inducible CRISPR nuclease (CRISPRn) knock in construct into the AAVS1 locusDepositorInsertsCas9
rtTA
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationPromoterCAG and TREAvailable sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
CRISPR-StAR4GN (Lenti)
Plasmid#222693PurposeCRISPR-screeningDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsGFPExpressionMammalianMutationPGK without BsmBI cutsitePromoterAvailable sinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2-sgAAVS1-HepmTurquoise2
Plasmid#192828PurposeExpresses sgRNA targeting AAVS1 (non-targeting control sgRNA for mouse) and mTurquoise2 from hepatocyte-specific promoterDepositorInsertmTurquoise2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 for sgRNA and hepatocyte-specific promoter (HS…Available sinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLCRISPR-CMV-cGAS3
Plasmid#102610PurposeCRISPR/Cas9 lentivector targeting human cGASDepositorInsertgRNA targeting human cGAS
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only