We narrowed to 14,003 results for: flag

1. Gal4-entry+NLS

   Plasmid

   #128013

   Purpose

   Vector containing the Gal4-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancer

   Depositor

   Julius Brennecke

   Article

   Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

   Insert

   Gal4 DBD-SV40NLS-3x-Flag
   

   Use

   Gal4 entry vector with nls to generate tethering …

   Tags

   Gal4-DBD-SV40NLS-3xFlag

   Expression

   Mutation

   Promoter

   Available since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDB-hCRY1-Halo-CD4-bla

   Plasmid

   #179445

   Purpose

   Donor vector to knock in Halotag C-terminal to human CRY1 gene

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   HaloTag (Halo7 Synthetic)
   

   Use

   CRISPR

   Tags

   His/Flag

   Expression

   Mutation

   Promoter

   None

   Available since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA 3.1 KBTBD4 R312RG

   Plasmid

   #184630

   Purpose

   Mammalian expression vector with N-terminal Flag for KBTBD4 R312RG expression in mammalian cells

   Depositor

   Vincenzo D'Angiolella

   Article

   Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

   Insert

   N-terminal 3xFlag tagged KBTBD4 R312RG (KBTBD4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R312RG

   Promoter

   CMV

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFastBac SpyTag-β2AR-SpyTag

   Plasmid

   #135620

   Purpose

   Expression of SpyTag-B2AR in insect cell

   Depositor

   Hee-Jung Choi

   Article

   Choi et al Science. 2019 Nov 29;366(6469):1150-1156

   Insert

   Beta 2 adrenergic receptor (ADRB2 Human)
   

   Use

   Tags

   HA-FLAG tag, Spytag, and eGFP-10xHis-R1D4 tag

   Expression

   Insect

   Mutation

   Deleted 1-24 aa, 366-413 aa

   Promoter

   Available since

   June 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-mGSDMD1-251

   Plasmid

   #111565

   Purpose

   Express ELANE-cleaved mouse GSDMD in mammalian cells

   Depositor

   Hongbo Luo

   Article

   Kambara et al Cell Rep. 2018 Mar 13;22(11):2924-2936.

   Insert

   Gasdermin D (Gsdmd Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

6. pRK-ATF4 1-275

   Plasmid

   #26118

   Depositor

   Yihong Ye

   Article

   Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

   Insert

   ATF4 (ATF4 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   aa's 276-351 have been deleted

   Promoter

   Available since

   March 1, 2011

   Availability

   Academic Institutions and Nonprofits only

7. U6-hGRIN2B-CAG-ps-SaCas9

   Plasmid

   #102853

   Purpose

   A single-chain light-controllable dSaCas9 with pdDronpa1 domains for hGRIN2B gene editing

   Depositor

   Michael Lin

   Article

   Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

   Insert

   sa-hGRIN2B-sgRNA; dSaCas9; pdDronpa1 (GRIN2B S. aureus, Human, Synthetic)
   

   Use

   CRISPR

   Tags

   3X Flag and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter;

   Available since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDEST40-CES2A-ΔC-S227A

   Plasmid

   #200556

   Purpose

   Mammalian expression of secreted mouse CES2A S227A mutant

   Depositor

   Jonathan Long

   Article

   Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

   Insert

   Mouse Carboxylesterase 2A S227A mutant delta C-terminal (Ces2a Mouse)
   

   Use

   Tags

   6xHis, Flag, and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDEST40-CES2A-ΔC

   Plasmid

   #200554

   Purpose

   Mammalian expression of secreted mouse CES2A

   Depositor

   Jonathan Long

   Article

   Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

   Insert

   Mouse Carboxylesterase 2A delta C-terminal (Ces2a Mouse)
   

   Use

   Tags

   6xHis, Flag, and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-Tbg-CES2A-ΔC

    Plasmid

    #200558

    Purpose

    Secreted mouse CES2A driven by heptaocyte-specific promoter in AAV viral vector

    Depositor

    Jonathan Long

    Article

    Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

    Insert

    Mouse Carboxylesterase 2A delta C-terminal (Ces2a Mouse)
    

    Use

    AAV

    Tags

    Flag

    Expression

    Mutation

    Promoter

    TBG promoter

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTBL469

    Plasmid

    #126458

    Purpose

    Expresses Cas9-15xFlag-TdT in mammalian cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    Cas9
    15x Flag
    TdT (DNTT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLenti-HF1RA-PGK-Puro

    Plasmid

    #110860

    Purpose

    Lentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-HF1RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    R661A, Q695A, Q926A, and NLS sequence at the N-te…

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. T7pr-His6-MBP-TEV-dCas9-BirA*

    Plasmid

    #159994

    Purpose

    Bacterial expression of dCas9-BirA*

    Depositor

    Jacob Corn

    Article

    Wang et al Mol Cell. 2020 Jul 16;79(2):221-233.e5.

    Insert

    dCas9-BirA*
    

    Use

    Tags

    6X-His, MBP, 3X-FLAG, NLS

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    T7

    Available since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRK-ATF4 111-351

    Plasmid

    #26119

    Depositor

    Yihong Ye

    Article

    Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

    Insert

    ATF4 (ATF4 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    aa's 1-110 have been deleted

    Promoter

    Available since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pRK-ATF4 248-351

    Plasmid

    #26120

    Depositor

    Yihong Ye

    Article

    Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

    Insert

    ATF4 (ATF4 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    ATF4 aa's 1-247 have been deleted

    Promoter

    Available since

    Jan. 31, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pTF-ACE2s

    Plasmid

    #162786

    Purpose

    The extracellular domain of Angiotensin converting enzyme 2 (ACE2) expression

    Depositor

    Ivan Vorobiev

    Article

    Kolesov et al Diagnostics (Basel). 2022 Feb 3;12(2). p

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    10xHis, FLAG, and hTPA leader

    Expression

    Mammalian

    Mutation

    Promoter

    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Exp-Rosa-EF1a-tTA-mCh-Rs1-TetO(sh)-2

    Plasmid

    #24419

    Depositor

    Edward Hsiao

    Article

    Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

    Insert

    RS1 (HTR4 Human)
    

    Use

    Mouse Targeting

    Tags

    FLAG and mCherry

    Expression

    Mutation

    Human 5HT4B receptor with D100A mutation, generat…

    Promoter

    Available since

    March 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti-HF1RA-P2A-Puro

    Plasmid

    #110853

    Purpose

    Lentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-HF1RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    R661A, Q695A, Q926A, and NLS sequence at the N-te…

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV10-mFoxP1-ES

    Plasmid

    #35169

    Depositor

    Benjamin Blencowe

    Article

    Gabut et al Cell. 2011 Sep 30;147(1):132-46. Epub 20

    Insert

    Foxp1 (Foxp1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pGEX6p-1-OSF-UFM1

    Plasmid

    #204565

    Purpose

    Expresses GST-Strep-FLAG-tagged UFM1 in E.coli

    Depositor

    Masaaki Komatsu

    Article

    Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

    Insert

    UFM1 (UFM1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    delta84-85aa

    Promoter

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. B3GALT5_pLENTI-CAG-IRES-GFP

    Plasmid

    #177006

    Purpose

    Mammalian lentiviral expression vector encoding B3GALT5

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    B3GALT5 (B3GALT5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDB-hPER2-Halo-CD4-bla

    Plasmid

    #179451

    Purpose

    Donor vector to knock in Halotag C-terminal to human PER2 gene

    Depositor

    Achim Kramer

    Article

    Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

    Insert

    HaloTag (Halo7 Synthetic)
    

    Use

    CRISPR

    Tags

    His/Flag

    Expression

    Mutation

    Promoter

    None

    Available since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDB-hCRY1-Luciferase-hvTK-bla

    Plasmid

    #179444

    Purpose

    Donor vector to knock in firefly Luciferase C-terminal to human CRY1 gene

    Depositor

    Achim Kramer

    Article

    Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

    Insert

    Luciferase
    

    Use

    CRISPR

    Tags

    His/Flag

    Expression

    Mutation

    Promoter

    None

    Available since

    Feb. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. KLC1-Myc

    Plasmid

    #166962

    Purpose

    Expresses FLAG-tagged SFPQ Y527A protein in pcDNA3.1

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    KLC1 (Klc1 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCDF1-MCS2-EF1-Puro-RGS7

    Plasmid

    #113728

    Purpose

    Express RGS7 in mamalian cells

    Depositor

    Yardena Samuels

    Article

    Qutob et al Sci Rep. 2018 Jan 12;8(1):653. doi: 10.1

    Insert

    RGS7 (RGS7 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLenti-VQRRA-P2A-Puro

    Plasmid

    #110851

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VQRRA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VQRRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    D1135V, R1335Q, T1337R and NLS sequence at the N-…

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDB-hCRY1-SNAP-CD4-bla

    Plasmid

    #179447

    Purpose

    Donor vector to knock in SNAPtag C-terminal to human CRY1 gene

    Depositor

    Achim Kramer

    Article

    Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

    Insert

    SnapTag
    

    Use

    CRISPR

    Tags

    His/Flag

    Expression

    Mutation

    Promoter

    None

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CHAF1B_pLENTI-CAG-IRES-GFP

    Plasmid

    #177009

    Purpose

    Mammalian lentiviral expression vector encoding CHAF1B

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    CHAF1B (CHAF1B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMSCVpuro-F-Gcn5-NLSm

    Plasmid

    #63708

    Purpose

    retroviral expression of cytoplasm-localized mouse Gcn5 in mammalian cells

    Depositor

    Kai Ge

    Article

    Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

    Insert

    Gcn5 (Kat2a Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    mutated K425, K427 and K438 to R (arginine)

    Promoter

    Available since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

30. pCAT526

    Plasmid

    #180513

    Purpose

    Plasmid expressing mammalian codon optimized wt PlmCasX, mNeonGreen, sgRNAv1 scaffold, and restriction sites to clone in new spacers

    Depositor

    Jennifer Doudna

    Article

    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

    Inserts

    CasX sgRNAv1
    PlmCasX-2A-mNeonGreen
    

    Use

    CRISPR

    Tags

    2x FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and U6

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. PGEX-DDX21-D2+C

    Plasmid

    #179243

    Purpose

    protein purification in E. coli

    Depositor

    Ling-Ling Chen

    Article

    Wu et al Science. 2021 Jul 30;373(6554):547-555.

    Insert

    DDX21-D2+C (DDX21 Human)
    

    Use

    Tags

    GST-flag

    Expression

    Bacterial

    Mutation

    aa396-782

    Promoter

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pTBL472

    Plasmid

    #126457

    Purpose

    Expresses Cas9-10xFlag-TdT in mammalian cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    Cas9
    10x Flag
    TdT (DNTT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Bxb1Donor_G102H_ADRB2_LucReporter

    Plasmid

    #129794

    Purpose

    encodes a donor vector for recombination with the Bxb1 recombinase with variant G102H of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene

    Depositor

    Sriram Kosuri

    Article

    Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1

    Insert

    ADRB2 (ADRB2 Human)
    

    Use

    Synthetic Biology

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    G102H

    Promoter

    N/A

    Available since

    Sept. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. CRAF_S29A_pcDNA3.1

    Plasmid

    #86506

    Purpose

    Expresses CRAF_S29A in mammalian cells [Edit]

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

    Insert

    CRAF (RAF1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S29A

    Promoter

    CMV

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLenti-VQRRA-P2A-GFP-PGK-Puro

    Plasmid

    #110864

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VQRRA-P2A-GFP in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VQRRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    D1135V, R1335Q, T1337R and NLS sequence at the N-…

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLenti-VQRRA-PGK-Puro

    Plasmid

    #110858

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VQRRA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VQRRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    D1135V, R1335Q, T1337R and NLS sequence at the N-…

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. KRAS-V14I

    Plasmid

    #154427

    Purpose

    V14I Kras with Flag tag inserted into N-terminal domain between 6Xhis tag and TEV cleavage site.

    Depositor

    Kenneth Westover

    Article

    Bera et al J Biol Chem. 2019 Sep 20;294(38):13964-1

    Insert

    KRAS (KRAS Human)
    

    Use

    Tags

    6xHis-tag and TEV protease cleavage sequence

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pfast bac cry1myc

    Plasmid

    #51892

    Purpose

    Backbone plasmid is pfastBacHTa from invitrogen

    Depositor

    Joseph Takahashi

    Article

    Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

    Insert

    cry1 (Cry1 Mouse)
    

    Use

    Tags

    3x Flag and myc

    Expression

    Mutation

    Promoter

    polyhedrin promoter

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

39. pCDF1-GRM3 (S610L)

    Plasmid

    #31800

    Depositor

    Yardena Samuels

    Article

    Prickett et al Nat Genet. 2011 Sep 25;43(11):1119-26. d

    Insert

    GRM3 (GRM3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed serine 610 to leucine

    Promoter

    CMV

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

40. pCDF1-GRM3 (E870K)

    Plasmid

    #31802

    Depositor

    Yardena Samuels

    Article

    Prickett et al Nat Genet. 2011 Sep 25;43(11):1119-26. d

    Insert

    GRM3 (GRM3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed glutamic acid 870 to lysine

    Promoter

    CMV

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

41. pCDF1-GRM3 (E767K)

    Plasmid

    #31801

    Depositor

    Yardena Samuels

    Article

    Prickett et al Nat Genet. 2011 Sep 25;43(11):1119-26. d

    Insert

    GRM3 (GRM3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed glutamic acid 767 to lysine

    Promoter

    CMV

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

42. pCDF1-GRM3 (G561E)

    Plasmid

    #31799

    Depositor

    Yardena Samuels

    Article

    Prickett et al Nat Genet. 2011 Sep 25;43(11):1119-26. d

    Insert

    GRM3 (GRM3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed glycine 561 to glutamic acid

    Promoter

    CMV

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

43. pLenti-VRERRA-PGK-Puro

    Plasmid

    #110859

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VRERRA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VRERRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    D1135V, G1218R, R1335E, T1337R, and NLS sequence …

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pLenti-VRERRA-P2A-GFP-PGK-Puro

    Plasmid

    #110865

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VRERRA-P2A-GFP in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VRERRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    D1135V, G1218R, R1335E, T1337R, and NLS sequence …

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pLenti-VRERRA-P2A-Puro

    Plasmid

    #110852

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VRERRA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VRERRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    D1135V, G1218R, R1335E, T1337R, and NLS sequence …

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. MSCV-NSD3-short del(100-263)

    Plasmid

    #72548

    Purpose

    expresses 3*FLAG tagged human NSD3-short with 100-263 aa deletion

    Depositor

    Christopher Vakoc

    Article

    Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

    Insert

    NSD3-short (NSD3 Human)
    

    Use

    Retroviral

    Tags

    3xFlag and SV40 NLS

    Expression

    Mammalian

    Mutation

    100-263 aa deletion

    Promoter

    LTR

    Available since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pcDNA5-F-Med26-K74A K75A

    Plasmid

    #49253

    Purpose

    Mammalian expression of flag-tagged human Med26-K74A K75A

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

    Insert

    Med 26 (MED26 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K74A K75A

    Promoter

    Available since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

48. pCIN4-EZOD

    Plasmid

    #44977

    Depositor

    Robert Oshima

    Article

    Galang et al J Biol Chem. 1996 Apr 5;271(14):7992-8.

    Insert

    Ets2 DBD (Ets2 Mouse)
    

    Use

    Tags

    FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Ets2 DNA binding domain (AA 332-468) only = domin…

    Promoter

    CMV

    Available since

    May 28, 2013

    Availability

    Academic Institutions and Nonprofits only

49. CtkA_300_cmv-10

    Plasmid

    #27434

    Depositor

    SeWon Suh

    Article

    Kim et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

    Insert

    Cell translocating kinas A (jhp0940 H. pylori)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    residues 301-325 are truncated

    Promoter

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

50. CtkA_D155Q/D179Q_cmv-10

    Plasmid

    #27435

    Depositor

    SeWon Suh

    Article

    Kim et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

    Insert

    Cell translocating kinas A (jhp0940 H. pylori)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D155Q/D179Q

    Promoter

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only