We narrowed to 8,227 results for: sars

1. pAF161

   Plasmid

   #135926

   Purpose

   All-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mCerulean3 fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).

   Depositor

   Andrew S. Flies

   Article

   Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab

   Insert

   TNFRSF9 fused to mCerulean3
   

   Use

   Transposon

   Tags

   mCerulean3

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDONR221-ZNF442

   Plasmid

   #101455

   Purpose

   Donor Vector containing ZNF442 transcription factor, part of the Human TFome Collection

   Depositor

   George Church

   Article

   Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

   Insert

   ZNF442 (ZNF442 Human)
   

   Use

   Gateway shuttling vector

   Tags

   Expression

   Mutation

   Last nucleotide of stop codon removed to allow fo…

   Promoter

   Available since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDONR223-ZNF366

   Plasmid

   #88019

   Purpose

   Donor Vector containing ZNF366 transcription factor, part of the Human TFome Collection

   Depositor

   Marc Vidal

   Article

   Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

   Insert

   ZNF366 (ZNF366 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Last nucleotide of stop codon removed to allow fo…

   Promoter

   Available since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDONR221 - myc ULK1 k46I

   Plasmid

   #27624

   Depositor

   Reuben Shaw

   Article

   Egan et al Science. 2010 Dec 23. ():.

   Insert

   mouse myc ULK1 K46I (Myc Mouse)
   

   Use

   Gateway donr vector

   Tags

   myc

   Expression

   Mutation

   Initial M from ULK1 removed. Silent mutation at A…

   Promoter

   Available since

   Feb. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

5. 426Gal-FUS-101-526aa-YFP

   Plasmid

   #29603

   Depositor

   Aaron Gitler

   Article

   Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

   Insert

   FUS-101-526aa (FUS Human)
   

   Use

   Tags

   YFP

   Expression

   Yeast

   Mutation

   Contains FUS aa# 101-526

   Promoter

   Available since

   July 20, 2011

   Availability

   Academic Institutions and Nonprofits only

6. 426Gal-FUS-1-453aa-YFP

   Plasmid

   #29600

   Depositor

   Aaron Gitler

   Article

   Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

   Insert

   FUS-1-453aa (FUS Human)
   

   Use

   Tags

   YFP

   Expression

   Yeast

   Mutation

   Contains FUS aa# 1-453

   Promoter

   Available since

   April 27, 2011

   Availability

   Academic Institutions and Nonprofits only

7. 426Gal-FUS-267-526aa-YFP

   Plasmid

   #29605

   Depositor

   Aaron Gitler

   Article

   Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

   Insert

   FUS-267-526aa (FUS Human)
   

   Use

   Tags

   YFP

   Expression

   Yeast

   Mutation

   Contains FUS aa# 267-526

   Promoter

   Available since

   April 27, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pDONR223-ZNF175

   Plasmid

   #88551

   Purpose

   Donor Vector containing ZNF175 transcription factor, part of the Human TFome Collection

   Depositor

   Marc Vidal

   Article

   Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

   Insert

   ZNF175 (ZNF175 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Last nucleotide of stop codon removed to allow fo…

   Promoter

   Available since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pVD1 Multiplexing Edit E4 (GB2241)

   Plasmid

   #160563

   Purpose

   tRNA and scaffold for the assembly of GBoligomers for position [4-5] of a polycistronic tRNA-gRNA.

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

   Insert

   Multiplexing Edit (E4)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FKBP-beta2(616-951)-GFP Double

    Plasmid

    #100729

    Purpose

    To express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    AP2B1 (AP2B1 Human)
    

    Use

    Tags

    FKBP and GFP

    Expression

    Mammalian

    Mutation

    Long isoform, deletion of clathrin box motif and …

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. FKBP-beta2(616-951)-GFP ∆CBM

    Plasmid

    #100728

    Purpose

    To express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    AP2B1 (AP2B1 Human)
    

    Use

    Tags

    FKBP and GFP

    Expression

    Mammalian

    Mutation

    Long isoform, deletion of clathrin box motif

    Promoter

    CMV

    Available since

    Sept. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 426Gal-FUS-285-526aa-YFP

    Plasmid

    #29606

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS-285-526aa (FUS Human)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Contains FUS aa# 285-526

    Promoter

    Available since

    Sept. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

13. 426Gal-FUS-1-270aa-YFP

    Plasmid

    #29597

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS-1-270aa (FUS Human)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Contains FUS aa# 1-270

    Promoter

    Available since

    July 18, 2011

    Availability

    Academic Institutions and Nonprofits only

14. 426Gal-FUS-368-526aa-YFP

    Plasmid

    #29607

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS-368-526aa (FUS Human)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Contains FUS aa# 368-526

    Promoter

    Available since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

15. MSCV-NSD3-short del(1-100)

    Plasmid

    #72547

    Purpose

    expresses 3*FLAG tagged human NSD3-short with 1-100 aa deletion

    Depositor

    Christopher Vakoc

    Article

    Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

    Insert

    NSD3-short (NSD3 Human)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    1-100 aa deletion

    Promoter

    LTR

    Available since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDONR221 - myc ULK1 4SA

    Plasmid

    #27625

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2010 Dec 23. ():.

    Insert

    mouse myc ULK1 4SA (Myc Mouse)
    

    Use

    Gateway donr vector

    Tags

    myc

    Expression

    Mutation

    S467A, S555A, T574A, S637A. Initial M from ULK1 r…

    Promoter

    Available since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pAF163

    Plasmid

    #135927

    Purpose

    All-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mNeptune2 fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).

    Depositor

    Andrew S. Flies

    Article

    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab

    Insert

    TNFRSF9 fused to mNeptune2
    

    Use

    Transposon

    Tags

    mNeptune2

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FKBP-beta2(616-951)-GFP Y215A

    Plasmid

    #100727

    Purpose

    To express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    AP2B1 (AP2B1 Human)
    

    Use

    Tags

    FKBP and GFP

    Expression

    Mammalian

    Mutation

    Long isoform Y215A mutation (Y815A in full length)

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3 FLAG FBXL2

    Plasmid

    #236455

    Purpose

    transient overexpression of FBXL2 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Kuchay et al Nature. 2017 Jun 22;546(7659):554-558. d

    Insert

    FBXL2 (FBXL2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFB-GST-FBXO22

    Plasmid

    #228367

    Purpose

    express human FBXO22 in insect cells, such as Trichoplusia ni Hi5

    Depositor

    Ning Zheng

    Article

    Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

    Insert

    FBXO22 (FBXO22 Human)
    

    Use

    Tags

    GST

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pFB-His-SKP1

    Plasmid

    #228368

    Purpose

    express human SKP1 in insect cells, such as Trichoplusia ni Hi5

    Depositor

    Ning Zheng

    Article

    Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

    Insert

    SKP1 (SKP1 Human)
    

    Use

    Tags

    His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pACE-MBP-FBXL17 (310–701)

    Plasmid

    #228369

    Purpose

    express human FBXL17 in insect cells, such as Trichoplusia ni Hi5

    Depositor

    Ning Zheng

    Article

    Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

    Insert

    FBXL17 (FBXL17 Human)
    

    Use

    Tags

    MBP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. FLAG-FBXL17

    Plasmid

    #236188

    Purpose

    Expression of N-terminal tagged FLAG-FBXL17 (H. sapiens, codon-optimized) in human cell lines.

    Depositor

    Michele Pagano

    Article

    Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

    Insert

    FLAG-FBXL17 (FBXL17 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1 HA-BACH1

    Plasmid

    #232269

    Purpose

    Expression of N-terminal tagged HA-BACH1 (H. sapiens) in human cell lines.

    Depositor

    Michele Pagano

    Article

    Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

    Insert

    BACH1 (BACH1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1 HA-FBXO22

    Plasmid

    #232271

    Purpose

    Expression of N-terminal tagged HA-FBXO22 (H. sapiens) in human cell lines

    Depositor

    Michele Pagano

    Article

    Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

    Insert

    FBXO22 (FBXO22 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAH33

    Plasmid

    #226850

    Purpose

    To constitutively overexpress Opa1 from the safe harbor locus Rosa26 in MEF

    Depositor

    Luke Chao

    Article

    Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

    Insert

    Opa1 (Opa1 Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRetroQ-GTSE1-acGFP-S91, 262, 454, 724A

    Plasmid

    #224741

    Purpose

    Retroviral vector for delivery and expression of acGFP1-tagged GTSE1 protein with S91, 262, 454, 724A mutation

    Depositor

    Michele Pagano

    Article

    García-Vázquez et al bioRxiv 2024.06.26.600797

    Insert

    GTSE1 (GTSE1 Human)
    

    Use

    Retroviral

    Tags

    acGFP

    Expression

    Mutation

    Serine 91, 262, 454, 724 to Alanine

    Promoter

    CMV

    Available since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRetroQ-GTSE1-acGFP-S91, 262, 454, 724D

    Plasmid

    #224742

    Purpose

    Retroviral vector for delivery and expression of acGFP1-tagged GTSE1 protein with S91, 262, 454, 724D mutation

    Depositor

    Michele Pagano

    Article

    García-Vázquez et al bioRxiv 2024.06.26.600797

    Insert

    GTSE1 (GTSE1 Human)
    

    Use

    Retroviral

    Tags

    acGFP

    Expression

    Mutation

    Serine 91, 262, 454, 724 Aspartate; Valine 53 Iso…

    Promoter

    CMV

    Available since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA1a_(CJT90)

    Plasmid

    #226989

    Purpose

    pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 1a must be used with gRNA 2a or 2b

    Depositor

    Benjamin Kleinstiver

    Article

    Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

    Insert

    SpCas9 gRNA 1a to create OPA1-del_exon5
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA2a_(CJT91)

    Plasmid

    #226991

    Purpose

    pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 2a must be used with gRNA 1a or 1b

    Depositor

    Benjamin Kleinstiver

    Article

    Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

    Insert

    SpCas9 gRNA 2a to create OPA1-del_exon5
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge