We narrowed to 99,754 results for: Ran

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pMM2-hPMCA4b

Plasmid

#47759

Purpose

mammalian expression of human PMCA4b

Depositor

John Penniston, Emanuel Strehler

Article

Adamo et al Biochem J. 1992 Aug 1;285 ( Pt 3):791-7.

Insert

PMCA4b (ATP2B4 Human)

Expression

Mammalian

Promoter

SV40

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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Arf Q71L CFP

Plasmid

#128149

Purpose

Expression of Arf Q71L

Depositor

Jennifer Lippincott-Schwartz

Article

Presley et al Nature. 2002 May 9;417(6885):187-93. doi

Insert

Arf1 (ARF1 Human)

Expression

Mammalian

Mutation

Q71L

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV E2F1 E132

Plasmid

#24224

Purpose

Mammalian expression of human E2F1 (DNA binding mutant) with HA tag

Depositor

Kristian Helin

Article

Helin et al Genes Dev. 1993 Oct . 7(10):1850-61.

Insert

E2F1 E132 (E2F1 Human)

Expression

Mammalian

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pDA093

Plasmid

#131390

Purpose

generation of Tet-On RPE

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

ORFeus

Expression

Mammalian

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-FLEx-TetOn3G-3xHA-FLEx

Plasmid

#133729

Purpose

Plasmid contains the floxed TetOn3G-transactivator sequence for expression of TetOn3G in the presence of Cre recombinase.

Depositor

Erin Schuman

Article

Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

Insert

TetOn3G-3xHA (floxed & inverted)

Use

AAV

Tags

3xHA-tag

Expression

Mammalian

Promoter

pCMV

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pAC153-dual-dCas9VP96-sgExpression

Plasmid

#48239

Purpose

Dual expression construct expressing both dCas9VP96 and sgRNA from separate promoters

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9

Use

CRISPR

Tags

HA-Tag, HA-tag, and VP96

Expression

Mammalian

Mutation

D10A H840A

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pIS1-Actb5UTR-renilla

Plasmid

#38234

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

Actb 5'UTR / Renilla (Actb Mouse)

Expression

Mammalian

Mutation

Actb 5' UTR fused to renilla ORF

Promoter

Endogenous Actb

Available Since

Nov. 7, 2012

Availability

Academic Institutions and Nonprofits only
pCAGGS-CD4-myc

Plasmid

#58537

Purpose

Expresses human CD4 with a C-terminal myc tag in mammalian cells

Depositor

Jacob Yount

Article

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

CD4 (CD4 Human)

Tags

myc

Expression

Mammalian

Available Since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only
C1-F-tractin-mCitrine

Plasmid

#155217

Purpose

Monitor global F-actin distribution

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

F-tractin (aa 9-52 of rat ITPKA) fused to mCitrine (Itpka Rat)

Tags

mCitrine

Expression

Mammalian

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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DIO-EF1a-FLEX-H2B-GFP-P2A-N2c(G)

Plasmid

#73476

Purpose

Encodes the G protein to enable transsynaptic RAV infection

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

H2B-GFP-P2A-N2c(G)

Use

AAV

Expression

Mammalian

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pBTK107

Plasmid

#110575

Purpose

BTK Type 2 promoter for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

CP25 Promoter with RBS

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pV92.2

Plasmid

#78409

Purpose

pKT230 plasmid engineered with the RTCas1v2-GFP cassette (RT-Cas1, Cas2, GFP) driven by the MMB-1 16S rRNA promoter, the CRISPR03 array driven by its native leader, and the tmRNA::td intron construct

Depositor

Andrew Fire

Article

Silas et al Science. 2016 Feb 26;351(6276):aad4234.

Inserts

RT-Cas1
Cas2

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pSG019

Plasmid

#126502

Purpose

Pseudomonas aeruginosa disaggregase ClpG cloned as ClpG-6xHis

Depositor

Ute Romling

Article

Lee et al Proc Natl Acad Sci U S A. 2018 Jan 9;115

Insert

disaggregase ClpG with 6xHis-tag

Tags

C-terminal 6xHis

Expression

Bacterial

Promoter

PBAD

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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LB701: pMVP/PB/mCherry-DEST

Plasmid

#121871

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ mCherry selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMRE154

Plasmid

#118504

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mOrange2

Expression

Bacterial

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pSB3K3 containing mRFP1

Plasmid

#118083

Purpose

For course in "Synthetic Biology: A Lab Manual"

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, mRFP1

Use

Escherichia coli

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pSB1K3 containing mRFP1

Plasmid

#118082

Purpose

For course in "Synthetic Biology: A Lab Manual"

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, mRFP1

Use

Escherichia coli

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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LC301: pMVP/PB/Zeo-DEST

Plasmid

#121877

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ Zeo selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR221-NFAT5

Plasmid

#79506

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human NFAT5 CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

NFAT5 (NFAT5 Human)

Use

Multisite gateway entry clone

Promoter

no Promoter

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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CMV/N3IC-HA

Plasmid

#47618

Purpose

in vitro transcription and translation of HA-tagged Notch3 Intracellular domain

Depositor

Urban Lendahl

Article

Lardelli et al Mech Dev. 1996 Oct;59(2):177-90.

Insert

Notch3 Intracellular domain (Notch3 Mouse)

Use

In vitro transcription and translation

Tags

HA

Expression

Mammalian

Mutation

codons 1664 (Met) to 2318 (Ala)

Promoter

CMV

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only