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We narrowed to 10,034 results for: Pol

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Showing: 9401 - 9440 of 10034 results

  2. pACEBAC1-CoREST-C

    Plasmid
    #109159
    Purpose
    Encodes a human C-terminal CoREST fragment with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression system
    Depositor
    Monika Golas
    Article
    Inui et al Protein Sci. 2017 May;26(5):997-1011. do
    Insert
    human C-terminal CoREST, sequence-optimized for insect cells (RCOR1 Human)
    Tags
    3xFLAG
    Expression
    Insect
    Promoter
    Polyhedrin
    Available Since
    Jan. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    109159-plasmid-map-sequence-id-232887

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  3. pOCC176

    Plasmid
    #118891
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal SNAP, cleavable with TEV
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-MBP-3C-SNAP-TEV-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS6-MBP, cleavable with 3C protease; SNAP, cleav…
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118891-plasmid-map-sequence-id-232282

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  7. pMuLE ENTR 7SK-miR-30 R4-R3

    Plasmid
    #62121
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Article
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Type
    Empty backbone
    Use
    Gateway Cloning and RNAi
    Expression
    Mammalian
    Available Since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62121-plasmid-map-sequence-id-101405

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  8. pMuLE ENTR 7SK-miR-30 L1-R5

    Plasmid
    #62118
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Article
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Type
    Empty backbone
    Use
    Gateway Cloning and RNAi
    Expression
    Mammalian
    Available Since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only

  10. pFAB1907

    Plasmid
    #47857
    Purpose
    BIOFAB reporter plasmid for measuring BBa_B1006 U10 termination efficiency
    Depositor
    Drew Endy
    Article
    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:
    Insert
    BBa_B1006 U10
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    Added two extra U at the end of Bba_1006 poly-U t…
    Promoter
    pLTetO12
    Available Since
    Dec. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    47857-plasmid-map-sequence-id-68389

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  24. pB PUb-integrase

    Plasmid
    #183966
    Purpose
    piggyBac plasmid for insect transformation, expressing PhiC31 integrase under control of the Aedes aegypti PUb promoter, GFP fluorescent marker
    Depositor
    Eric Marois
    Article
    Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:
    Insert
    PhiC31 integrase
    Use
    Piggybac insect transgenesis destination vector
    Promoter
    Aedes aegypti Polyubiquitin
    Available Since
    Aug. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    183966-plasmid-map-sequence-id-361316

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  25. pSLQ10873

    Plasmid
    #183961
    Purpose
    U6-SKSKSO(crRNA array)-CAG-hyperdCas12a-HA-miniVPR
    Depositor
    Stanley Qi
    Article
    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d
    Inserts
    HyperdCas12a
    poly-crRNA array targeting Sox2-Klf4-Oct4
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D832A, D156R, E292R, D235R, D350R
    Promoter
    CAG and U6
    Available Since
    Aug. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    183961-plasmid-map-sequence-id-369669

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  28. pCsy_complex

    Plasmid
    #89232
    Purpose
    Plasmid contains the genes Csy1, Csy2, Csy3, Csy4, cloned as a polycistronic operon from bacterium Pseudomonas aeruginosa PA14. Csy3 includes an N-terminal 6his tag and TEV protease cleavage site
    Depositor
    Blake Wiedenheft
    Article
    Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:
    Inserts
    Csy1
    Csy2
    Csy3
    Csy4
    Tags
    6xHis-TEV protease
    Available Since
    April 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    89232-plasmid-map-sequence-id-177172

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  32. pSL3718 (pQCascade_Pse)

    Plasmid
    #200899
    Purpose
    Expresses human codon-optimized Pseudoalteromonas Cas7, Cas8, Cas6, and TniQ from a CMV promoter, in a polycistronic vector format separated by T2A sequences.
    Depositor
    Samuel H. Sternberg
    Article
    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103
    Insert
    PseCas7, PseCas8, PseCas6, PseTniQ
    Tags
    NLS (TniQ) and NLS, T2A (Cas7, Cas8, Cas6)
    Expression
    Mammalian
    Available Since
    May 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    200899-plasmid-map-sequence-id-393105

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  35. Fireworks GFP[PTC-] (AVA2598)

    Plasmid
    #85445
    Purpose
    Expresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a beta-globin reporter.
    Depositor
    Joan Steitz
    Article
    Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:
    Insert
    tDNA1 EF1alfa-5xEGFP-TEVprotease-PEST-beta-globin(dI1)-BGH polyA tDNA2 FRT-PEST-TEVsite-PuromycinR
    Use
    Minimal backbone fragment for low-copy bacterial …
    Expression
    Mammalian
    Available Since
    Feb. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    85445-plasmid-map-sequence-id-171491

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  37. GST-mDia1(FH1FH2DAD)-His

    Plasmid
    #85822
    Purpose
    contains the formin homolgy domains: FH2 is required for nucleation, processive tracking of barbed-end of actin filaments ; FH1 permits enhancement of actin polymerization rate.
    Depositor
    Antoine Jégou, Guillaume Romet-Lemonne
    Article
    Jegou et al Nat Commun. 2013;4:1883. doi: 10.1038/nc
    Insert
    Protein diaphanous homolog 1 (Diaph1 Mouse)
    Tags
    8*His and GST
    Expression
    Bacterial
    Promoter
    tac
    Available Since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    85822-plasmid-map-sequence-id-183287

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  38. pCAGGs-NLS-TIR1_P2A_NES-TIR1

    Plasmid
    #117699
    Purpose
    Expression of NLS-(Os)TIR1-HA and Myc-NES-(Os)TIR1from one mRNA
    Depositor
    Joerg Mansfeld
    Article
    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:
    Inserts
    TRANSPORT INHIBITOR RESPONSE 1
    TRANSPORT INHIBITOR RESPONSE 1
    Tags
    HA tag, Myc-NES, and NLS
    Expression
    Mammalian
    Mutation
    codon optimized for murine expression
    Promoter
    None, 2A peptide from porcine teschovirus-1 polyp…
    Available Since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    117699-plasmid-map-sequence-id-368109

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Showing: 9401 - 9440 of 10034 results