We narrowed to 59,446 results for: cloning

1. KA901: pMVP (L3-L2) HA tag + pA; EF1a::eGFP-P2A-TETa-pA

   Plasmid

   #121804

   Purpose

   pMVP L3-L2 entry plasmid, contains HA tag-polyA + EF1a-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream EF1a-driven GFP-P2A-TETa

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   HA epitope tag-polyA + EF1a::eGFP-P2A-TETa-polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. KA701: pMVP (L3-L2) HA tag + pA; RIP::eGFP-P2A-TETa-pA

   Plasmid

   #121808

   Purpose

   pMVP L3-L2 entry plasmid, contains HA tag-polyA + RIP-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream RIP-driven GFP-P2A-TETa

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   HA epitope tag-polyA + RIP::eGFP-P2A-TETa-polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHCD1E

   Plasmid

   #102956

   Purpose

   gene cloning of human CD1E

   Depositor

   Gennaro De Libero, Lucia Mori

   Article

   Facciotti et al Proc Natl Acad Sci U S A. 2011 Aug 23;10

   Insert

   CD1E cDNA (CD1E Human)
   

   Use

   Cloning

   Available Since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCR8/GW/TOPO-ELK1Y158A

   Plasmid

   #98628

   Purpose

   Gateway entry TOPO TA cloning vector ELK1 Y158A

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   ELK1 Y158A (Elk1 Mouse)
   

   Use

   Topo ta cloning vector

   Mutation

   Y158A

   Available Since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. JC Mad-1

   Plasmid

   #25626

   Depositor

   Peter Howley

   Article

   Howley et al J Virol. 1980 Dec . 36(3):878-82.

   Insert

   JC Virus Mad-1
   

   Expression

   Bacterial

   Available Since

   Oct. 20, 2011

   Availability

   Academic Institutions and Nonprofits only

6. ultra CAST

   Plasmid

   #236111

   Purpose

   Encodes sfGFP BbsI dropout for cloning in guide RNA sequence, encodes mScarlet BsmBI/BsaI dropout for cloning in cargo sequence

   Depositor

   Jeffrey Barrick

   Article

   VanDieren et al MicroPubl Biol. 2025 Aug 2;2025:10.17912

   Type

   Empty backbone

   Available Since

   July 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHIV-EGFP

   Plasmid

   #21373

   Purpose

   self-inactivating lentiviral plasmid for co-expression of your gene of interest and EGFP

   Depositor

   Bryan Welm, Zena Werb

   Article

   Welm et al Cell Stem Cell. 2008 Jan 10. 2(1):90-102

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 19, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p663-UBC-miniTurbo-V5-MAST3_IDG-K

   Plasmid

   #198007

   Purpose

   Gateway destination clone of MAST3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-MAST3 (MAST3 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mTagBFP2-pBAD

   Plasmid

   #54572

   Purpose

   Localization: Protein Expression Vector , Excitation: 399, Emission: 456

   Depositor

   Michael Davidson

   Article

   Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Tags

   6xHis, T7, and mTagBFP2

   Expression

   Bacterial

   Available Since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. phU6-gRNA

    Plasmid

    #53188

    Purpose

    Expresses the S. pyogenes sgRNA from the human U6 promoter

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pU6-As-crRNA

    Plasmid

    #78956

    Purpose

    Cloning vector for expression of AsCpf1 crRNA. It contains BsmB1 site for cloning.

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    June 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. phH1-gRNA

    Plasmid

    #53186

    Purpose

    Expresses the S. pyogenes sgRNA from the H1 promoter

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    human H1

    Available Since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pmU6-gRNA

    Plasmid

    #53187

    Purpose

    Expresses the S. pyogenes sgRNA from the mouse U6 promoter

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    mouse U6

    Available Since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ph7SK-gRNA

    Plasmid

    #53189

    Purpose

    Expresses the S. pyogenes sgRNA from the 7SK promoter

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    human 7SK

    Available Since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAdEasy-1

    Plasmid

    #16400

    Purpose

    Adenoviral vector for use in AdEasy System.

    Depositor

    Bert Vogelstein

    Article

    He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Adenoviral

    Expression

    Mammalian

    Available Since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti SpBsmBI sgRNA Hygro

    Plasmid

    #62205

    Purpose

    An empty sgRNA expression vector for expression of sgRNA for Sp Cas9

    Depositor

    Rene Maehr

    Article

    Pham et al Methods Mol Biol. 2016;1358:43-57. doi:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA5/FRT/TO GFP

    Plasmid

    #19444

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    GFP
    

    Expression

    Mammalian

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. piRFP670-N1

    Plasmid

    #45457

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Has Service

    Cloning Grade DNA

    Insert

    iRFP670
    

    Expression

    Mammalian

    Mutation

    T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

    Promoter

    CMV

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUC18T-mini-Tn7T-Gm

    Plasmid

    #63121

    Purpose

    mini-Tn7 vector

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    May 17, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pCZGY546

    Plasmid

    #90466

    Purpose

    Pmec-4::mcherry, Expresses mcherry red fluorescence in C. elegans touch neurons

    Depositor

    Andrew Chisholm

    Article

    Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

    Insert

    Pmec-4-mcherry
    

    Tags

    mcherry

    Expression

    Worm

    Promoter

    Pmec-4

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge