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pBK-EQR-BE3

Plasmid

#85172

Purpose

Expresses EQR-BE3 in mammalian cells

Depositor

David Liu

Article

Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Insert

EQR-BE3

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pCB036 FoMV-sgGFP

Plasmid

#234813

Purpose

To express gRNA from Foxtail Mosaic Virus Vector targeting GFP (T-DNA)

Depositor

Daniel Voytas

Article

Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

Insert

gRNA targeting GFP

Expression

Plant

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQ154

Plasmid

#69305

Purpose

Gateway entry vector with AteCas9 (Arabidopsis codon optimized)

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

AteCas9 (Arabidopsis codon optimized)

Use

CRISPR

Mutation

NLS fusion

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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eSpCas9(1.1)_No_FLAG

Plasmid

#79877

Purpose

FLAGless construct expressing high specificity eSpCas9(1.1). Px330-like plasmid.

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Type

Empty backbone

Use

CRISPR

Tags

Untagged eSpCas9(1.1)

Expression

Mammalian

Promoter

CBh

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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Hygro-iDEST

Plasmid

#75339

Purpose

GATEWAY destination vector for site-specific integration into the human AAVS1/PPP1R12C locus for doxycycline-inducible gene over-expression

Depositor

Danwei Huangfu

Article

Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

Insert

TRE-DEST

Expression

Mammalian

Promoter

TRE-tight

Available Since

May 24, 2016

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB1-2-PT-SA-SD-0-2xTY1-V5

Plasmid

#61255

Purpose

CRISPR-RMCE, second step attB plasmid, intron phase 0, exchange dsRed with 2xTY1 and V5 tags cassette

Depositor

Frank Schnorrer

Article

Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

Insert

2xTY1-V5

Use

CRISPR

Tags

TY1 and V5

Available Since

Nov. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pYPQ230-D156R (ttLbCas12a)

Plasmid

#195366

Purpose

A temperature-tolerant LbCas12a mutant (D156R), also known as ttLbCas12a

Depositor

Yiping Qi

Article

Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

Insert

LbCas12a-D156R (ttLbCas12a)

Use

CRISPR; Gateway compatible lbcas12a-d156r (ttlbca…

Expression

Plant

Promoter

ZmUBI1

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pPP076

Plasmid

#158794

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-1 in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-1

Expression

Bacterial

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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Sox1/2/3-2373/+3>nls::Cas9::nls

Plasmid

#59989

Purpose

Sox1/2/3 (SoxB1) driver driving nls::Cas9::nls

Depositor

Lionel Christiaen

Article

Stolfi et al Development. 2014 Nov;141(21):4115-20. d

Insert

nls::Cas9::nls

Use

CRISPR

Mutation

Reverted mutations from dCas9 to wiltdtype

Promoter

Ciinte.Sox1/2/3 (SoxB1) -2373/+3

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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gh22

Plasmid

#106695

Purpose

expression of gRNA targeting COSMC

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

COSMC

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only
PX458_ZNF644_2

Plasmid

#86306

Purpose

Encodes gRNA for 3' target of human ZNF644

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ZNF644 (ZNF644 Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_ZNF644_1

Plasmid

#86305

Purpose

Encodes gRNA for 3' target of human ZNF644

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ZNF644 (ZNF644 Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A1511

Plasmid

#91037

Purpose

Module A, Promoter: OsAct1, Gene: Csy4-P2A-TaCas9, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-TaCas9

Use

CRISPR

Promoter

OsAct1

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only
pTRANS_213

Plasmid

#91112

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: WDV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pH-PABE-7-sgRNA

Plasmid

#115621

Purpose

Targeted A to G in rice

Depositor

Caixia Gao

Article

Li et al Genome Biol. 2018 May 29;19(1):59. doi:

Insert

wtTadA-TadA7.10-nCas9-3*NLS

Use

CRISPR

Expression

Plant

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pBBK

Plasmid

#62670

Purpose

Vector for PhiC31o mRNA synthesis

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Insert

PhiC31

Use

Synthetic Biology

Expression

Mammalian

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pMG343

Plasmid

#176183

Purpose

preassembled yeast MoClo level-1 backbone with toxic gene dropout cassette (ccdB and TPK2) and URA3 marker

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

TPK2+ccdB dropout cassette

Expression

Yeast

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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LbdCas12a KRAB

Plasmid

#188503

Purpose

Expresses FLAG tagged LbdCas12a KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D832A

Promoter

EF1a

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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PU6::klp-12_sgRNA

Plasmid

#46170

Purpose

klp-12 targeting sgRNA

Depositor

John Calarco

Article

Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

Insert

klp-12 targeting sgRNA (klp-12 Synthetic)

Use

CRISPR

Expression

Worm

Promoter

C. elegans U6 snRNA pol III promoter

Available Since

July 1, 2013

Availability

Academic Institutions and Nonprofits only

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pMOD_A1510

Plasmid

#91036

Purpose

Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-TaCas9

Use

CRISPR

Promoter

ZmUbi

Available Since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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