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pXWPrinA-gfp2

Plasmid

#123147

Purpose

pSB1K3 carrying PrinA-30gfp-t

Depositor

Baojun Wang

Article

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

PrinA-30gfp-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

PrinA

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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Double UP mNeon to mScarlet FRT

Plasmid

#141111

Purpose

Natively produces mNeonGreen, in the presence of Flp or Flpe will be recombined to produce mScarlet.

Depositor

Erik Dent

Article

Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d

Type

Empty backbone

Expression

Mammalian

Promoter

CAG

Available Since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

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pDWJ26

Plasmid

#207603

Purpose

GFP-AIA expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-AIA

Expression

Bacterial

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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PKC iota CAT

Plasmid

#21254

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC iota (PRKCI Human)

Expression

Mammalian

Mutation

delta 1-231

Available Since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
pHN1_lacUV5-mNG-PDT

Plasmid

#165583

Purpose

Expresses mNeonGreen and C-terminally fused PDT

Depositor

Daniel Ducat

Article

Sakkos et al ACS Synth Biol. 2021 Jul 16;10(7):1667-1

Insert

mNeonGreen-PDT

Tags

Protein degradation tag

Expression

Bacterial

Promoter

Ptrc

Available Since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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FRP2372

Plasmid

#127578

Purpose

Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Lys2 selection marker

Depositor

Joerg Stelling

Article

Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

Insert

PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1

Use

Synthetic Biology; Shuttle vector

Expression

Yeast

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pJMP8045

Plasmid

#220900

Purpose

Tn7 transposase (tnsABCD) expression plasmid for Rhodobacter sphaeroides

Depositor

Jason Peters

Article

Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

Insert

tnsABCD

Expression

Bacterial

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCRL1

Plasmid

#153997

Purpose

sgRNA in vitro transcription plasmid.

Depositor

Shengdar Tsai

Article

Lazzarotto et al Nat Protoc. 2018 Nov;13(11):2615-2642. d

Type

Empty backbone

Use

CRISPR; Sgrna under a t7 promoter for in vitro tr…

Promoter

T7

Available Since

June 29, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2+Lifeact-eGFP

Plasmid

#128428

Purpose

Contains insert allowing in-vivo visualization of F-actin

Depositor

Rusty Lansford

Article

Tran et al J Vis Exp. 2019 Jun 7;(148). doi: 10.379

Insert

Lifeact-eGFP

Expression

Mammalian

Promoter

CMV & SP6

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-ChR2-GFP

Plasmid

#58881

Purpose

AAV expression of ChR2-GFP under the Syn promoter

Depositor

Edward Boyden

Article

Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.

Insert

ChR2-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

Syn

Available Since

Oct. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pRVdG-4mCherry

Plasmid

#52488

Purpose

Expresses mCherry

Depositor

Sebastian Seung

Article

Weible et al J Neurosci. 2010 Dec 8;30(49):16509-13.

Insert

mCherry

Use

Rabies virus

Promoter

CMV

Available Since

Aug. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pACSA_PcptOO_C38T,A39T_YFP_PMB2_LacIWF

Plasmid

#82021

Purpose

cLac143; pALM143; IPTG inducible system

Depositor

Brian Pfleger

Article

Markley et al ACS Synth Biol. 2015 May 15;4(5):595-603

Insert

YFP

Promoter

Pcptoo_C38T,A39T

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pFCK(1.3)GFP-SynaptophysW

Plasmid

#27235

Depositor

Pavel Osten

Article

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

EGFP

Use

Lentiviral

Tags

Synaptophys-GFP

Available Since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

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plvx-mTSPAN9-mCherry

Plasmid

#210337

Purpose

Overexpression of mTSPAN9

Depositor

Li Yu

Article

Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

Insert

mTSPAN9 (Tspan9 Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTYF-1xGfaABC1D-EGFP

Plasmid

#19974

Depositor

Sergey Kasparov

Article

Liu et al BMC Biotechnol. 2008 May 16;8:49. doi: 1

Insert

GfaABC1D-EGFP

Expression

Mammalian

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pNB2176

Plasmid

#113395

Purpose

Ty3-IN-GFP; alpha factor inducible

Depositor

Suzanne Sandmeyer

Article

Beliakova-Bethell et al RNA. 2006 Jan;12(1):94-101. doi: 10.1261

Insert

Ty3 with integrase-green fluorescent protein

Tags

IN-GFP

Expression

Yeast

Available Since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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HS-mKate2

Plasmid

#105945

Purpose

heat shock inducible transgenesis, cytosolic mKate2

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

mKate2

Expression

Bacterial

Available Since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCM291rfp

Plasmid

#59775

Purpose

pCMrfp with TrfA mutation (291E to G)

Depositor

Nathan Hillson

Article

Bi et al Microb Cell Fact. 2013 Nov 13;12:107. do

Insert

rfp

Expression

Bacterial

Promoter

pBAD

Available Since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

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EZ-L545

Plasmid

#173024

Purpose

Encodes OptoEXP2 circuit

Depositor

Jose Avalos

Article

Zhao et al ACS Synth Biol. 2021 May 21;10(5):1143-1

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB-PhoCl

Plasmid

#87688

Purpose

Express PhoCl in E. coli

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

PhoCl

Tags

6xHis

Expression

Bacterial

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pKRR5

Plasmid

#149468

Purpose

Broad-Host range expression vector, RK2 and pUC origin, RhaR-RhaS activator, PrhaBAD promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

RhaS-RhaR-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PrhaBAD

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pJKR-L-acuR

Plasmid

#62568

Purpose

Acrylate biosensor. Low copy plasmid that expresses GFP proportional to acrylate concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pGC185

Plasmid

#19624

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Prpl-28<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)<4xNLS-tdimer2(12)::let-858(3’)

Use

Flp/frt

Expression

Worm

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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HS-Ras-mKate2

Plasmid

#105951

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

Ras

Tags

mKate2

Expression

Bacterial

Available Since

Feb. 16, 2018

Availability

Academic Institutions and Nonprofits only

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EZ-L580

Plasmid

#173023

Purpose

Encodes OptoAMP4 circuit

Depositor

Jose Avalos

Article

Zhao et al ACS Synth Biol. 2021 May 21;10(5):1143-1

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Mutation

A79Q

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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FUW-SM

Plasmid

#20330

Depositor

Rudolf Jaenisch

Article

Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

Sox2-F2A-cMyc

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pRSETb-tVYN-TmΔ

Plasmid

#149694

Purpose

High-copy plasmid to express the tVYN-TmΔ luminescent biosensor for in vitro purification

Depositor

Ming Hammond

Article

Dippel et al ACS Chem Biol. 2020 Mar 25. doi: 10.1021

Insert

VenusΔC12-TmYcgRΔ-NLucΔ4

Expression

Bacterial

Promoter

T7

Available Since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pJKR-L-cdaR

Plasmid

#62558

Purpose

Glucarate biosensor. Low copy plasmid that expresses GFP proportional to glucarate concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pESC-URA-Ub-G76A-Rnq1-GFP

Plasmid

#21052

Depositor

Judith Frydman

Article

Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

Insert

Rnq1 (RNQ1 Budding Yeast)

Use

Yeast/bacteria shuttle

Tags

GFP and ubiquitin

Expression

Yeast

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only
pJZC533

Plasmid

#135435

Purpose

Yeast integrating plasmid for pTEF1-mCherry reporter gene

Depositor

Jesse Zalatan

Article

Cunningham-Bryant et al Chembiochem. 2019 Jun 14;20(12):1519-152

Insert

mCherry

Expression

Yeast

Mutation

codon optimized for S. cerevisiae

Promoter

pTEF1

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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HfACR1_EYFP_pcDNA3.1

Plasmid

#161026

Purpose

Expresses strongly red-shifted HfACR1 in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

Insert

HfACR1

Tags

EYFP

Expression

Mammalian

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pUK

Plasmid

#64185

Purpose

harbours marker genes URA3 and LYS2

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

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10xUAS-GFP-DD

Plasmid

#104989

Purpose

Expresses GFP fused with destabilizing domain (DD) downstream of a 10XUAS sequence.

Depositor

Jing-W Wang

Article

Sethi et al Elife. 2017 Nov 15;6. pii: e30327. doi:

Insert

TMP-inducible destabilized GFP (GFP-DD)

Tags

DD

Expression

Insect

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pESC-URA-Rnq1-GFP

Plasmid

#21051

Depositor

Judith Frydman

Article

Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

Insert

Rnq1 (RNQ1 Budding Yeast)

Use

Yeast/bacteria shuttle

Tags

GFP

Expression

Yeast

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only
pFT1.0

Plasmid

#46138

Depositor

Wenbiao Chen

Article

Ni et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

mCherry

Use

Cre/Lox; Mutagenic gene trap for zebrafish

Available Since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pLK

Plasmid

#64186

Purpose

harbours marker genes LEU2 and LYS2

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pRL488

Plasmid

#70263

Purpose

designed to facilitate transcriptional fusions to luxAB, encoding luciferase, on a plasmid that can replicate in both Escherichia coli and strains of filamentous cyanobacteria.

Depositor

Peter Wolk

Article

Elhai et al EMBO J. 1990 Oct;9(10):3379-88.

Type

Empty backbone

Use

Luciferase; Cyanobacteria expression

Tags

luxAB

Promoter

none

Available Since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
CMVTO-SEAP-26Sfur-3TM-hcvs-KKMP

Plasmid

#179635

Purpose

HCVP-inducible tri-transmembrane SEAP RELEASE construct

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

SEAP RELEASE

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CMVTO-SEAP-26Sfur-B2AD-hcvs-KKMP

Plasmid

#179638

Purpose

HCVP-inducible single TM SEAP RELEASE construct

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

SEAP RELEASE

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAL-c2x-MT3

Plasmid

#32115

Depositor

David DeRosier, Chris Mercogliano, Cristina Risco Ortiz

Article

Diestra et al J Struct Biol. 2009 Mar;165(3):157-68. E

Insert

MT (3x) (Mt1 Mouse)

Tags

MBP

Expression

Bacterial

Promoter

P-lac

Available Since

Jan. 13, 2012

Availability

Academic Institutions and Nonprofits only

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