We narrowed to 8,603 results for: sars
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Plasmid#140020PurposePlasmid with 4xMmaPylT cassette and chimeric Mma/Mba PylRS for amber suppression and incorporation of photo-crosslinking-lysine; for transient or stable piggyBac-mediated integrationDepositorInsertAbKRS-chIPYE (pylS Methanosarcina mazei and Methanosarcina bakeri)
TagsFLAGExpressionMammalianMutation1-149 MbPylRS IPYE (V31I, T56P, H62Y, A100E) + 18…PromoterEF1Available SinceJune 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-MTS-TagBFP-P2AT2A-EGFP-NLS-P2AT2A-mCherry-PTS1
Plasmid#87829PurposeHigh-efficient mammalian expression vector for co-expression of BFP-, EGFP- and mCherry-tagged proteins using P2AT2A. MTS, NLS and PTS1 can be replaced by proteins of interest.DepositorInsertsMTS
NLS
PTS1
ExpressionMammalianPromoterCMV promoter, tetracycline operatorAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
lenti-HA-mBec1 F121A
Plasmid#99506PurposeLentiviral expression of mutant Becn1 leading to hyperactive autophagyDepositorAvailable SinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE311B
Plasmid#120418PurposeExpresses Lambda Beta and a dominant negative MutL E32K allele, controlled by XylS-Pm expression system for high precision and efficient MAGE experiments at 37°C. RSF1010 Ori; Broad host-range; KanRDepositorArticleInsertsMutL E32K
Lambda Beta
XylS
ExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…PromoterPmAvailable SinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ shSTING-Hsa
Plasmid#128158PurposeDoxycyclin inducible shRNA knockdown of human STING geneDepositorAvailable SinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-NICD
Plasmid#61540PurposeExpresses NOTCH-ICD in mammalian cellsDepositorInsertNOTCH-ICD (NOTCH1 Human)
UseLentiviralExpressionMammalianMutationNOTCH intracellular domain (aa1761-2555); A1943S…Available SinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
TOM20*-SBP-GFP
Plasmid#120173PurposeExpresses a chimera of the mitochondria-targeting signal of TOM20, SBP tag and GFP (fluorescent tag)DepositorInsertMitochondrial import receptor subunit TOM20 homolog (TOMM20 Human)
TagsSBP and eGFPExpressionMammalianMutationTruncated TOM20: 1-30 aaPromoterCMVAvailable SinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUB6-HuMGF
Plasmid#190782PurposeExpresses membrane-bound form of human SCF in mammalian cellsDepositorAvailable SinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLox-LV-pCMV-FLPo-pGK-mCl-GP33/66
Plasmid#216482PurposeExpresses a lox-p flanked FLPo and mClover3 fluorescent protein with LMCV GP33-43 and GP66-77 epitopes embedded in loop of mclover for initiating and removing immunogenic tumor cells in KPFrt miceDepositorInsertsFLPo Recombinase
mClover3-GP33/66
UseLentiviralPromoterpCMV and pGKAvailable SinceOct. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hGPR56-IRES-GFP
Plasmid#52297PurposeExpresses human GPR56 and GFP in mammalian cells.DepositorAvailable SinceApril 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF280C
Plasmid#88658PurposeDonor Vector containing ZNF280C transcription factor, part of the Human TFome CollectionDepositorInsertZNF280C (ZNF280C Human)
UseGateway donor vectorMutationLast nucleotide of stop codon removed to allow fo…Available SinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Coff/Fon DREADD Gi-mCherry
Plasmid#177669PurposeExpresses DREADD hM4D (Gi) only in Flp expressing cells and not in Cre expressing cellsDepositorInsertDREADD Gi-mCherry
UseAAV, Cre/Lox, and Mouse Targeting ; IntrsectTagsmCherryExpressionMammalianPromoternEFAvailable SinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3-OGDH_wobble
Plasmid#110938PurposeOGDH ORF containing silent wobble mutations insensitive to corresponding shRNAsDepositorInsertOGDH wobble cDNA (OGDH Human)
UseLentiviralMutation15 silent wobble mutations corresponding to three…PromoterSV40Available SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgCD71-2
Plasmid#46918PurposeHuman pSico-based U6 vector containing murine U6 promoter and sgRNA targeting endogenous CD71 geneDepositorInsertsUseCRISPR and LentiviralExpressionMammalianPromoterCMV and U6Available SinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_Dual-GST-TEV-SINTBAD-EGFP (codon optimized Sf9)
Plasmid#198035PurposeUsed for the expression and purification of EGFP labelled SINTBAD (SMC Internal No. 1613)DepositorAvailable SinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ZNF208
Plasmid#101549PurposeDonor Vector containing ZNF208 transcription factor, part of the Human TFome CollectionDepositorInsertZNF208 (ZNF208 Human)
UseGateway shuttling vectorMutationLast nucleotide of stop codon removed to allow fo…Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCh-KIF17*-strep
Plasmid#120167PurposeExpresses a chimera of mCherry (fluorescent tag), a KIF17-based anterograde motor and streptavidinDepositorInsertkinesin family member 17 (KIF17 Human)
TagsStreptavidin and mCherryExpressionMammalianMutationTruncated KIF17: 1-509 aaPromoterCMVAvailable SinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCh-KIF13A*-strep
Plasmid#120166PurposeExpresses a chimera of mCherry (fluorescent tag), a KIF13A-based anterograde motor and streptavidinDepositorInsertkinesin family member 13A (KIF13A Human)
TagsStreptavidin and mCherryExpressionMammalianMutationTruncated KIF13A: 1-639 aaPromoterCMVAvailable SinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-SBP-VAP-A*
Plasmid#120175PurposeExpresses a chimera of GFP (fluorescent tag), SBP tag and the C-terminal of ER-resident protein VAP-ADepositorInsertVAMP associated protein A (VAPA Human)
TagsEGFP and SBPExpressionMammalianMutationTruncated VAP-A: 118-249PromoterCMVAvailable SinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
mini-YFP-hDF-C2F-TM
Plasmid#72600Purposemini human dysferlin (from domain F to transmembrane ) tagged with YFPDepositorInsertdysferlin (DYSF Human)
TagsEYFPExpressionMammalianMutationfrom domain F to transmembrane domainPromoterCMVAvailable SinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLOC-MCOLN1
Plasmid#131583PurposeFor expression of human MCOLN1 in mammalian cells, GFP-taggedDepositorAvailable SinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCh-KIF5A*-strep
Plasmid#120163PurposeExpresses a chimera of mCherry (fluorescent tag), a KIF5A-based anterograde motor and streptavidinDepositorInsertkinesin family member 5A (Kif5a Mouse)
TagsStreptavidin and mCherryExpressionMammalianMutationTruncated KIF5A: 1-559 aaPromoterCMVAvailable SinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVD1 Multiplexing Edit E3-E4-En-1 (GB2244)
Plasmid#160566PurposeGBoligomers for the position [3_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.DepositorInsertMultiplexing Edit (E3-E4-En-1)
UseCRISPR and Synthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 His-GFP-EWSR1-myc-His
Plasmid#46385Purposeexpresses EGFP tagged EWSR1 in mammalian cellsDepositorAvailable SinceSept. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF846
Plasmid#88355PurposeDonor Vector containing ZNF846 transcription factor, part of the Human TFome CollectionDepositorInsertZNF846 (ZNF846 Human)
UseGateway donor vectorMutationLast nucleotide of stop codon removed to allow fo…Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1_6xHis-TEV-mCh-NDP52
Plasmid#187829PurposePlasmid for the expression and purification of 6xHis-TEV-mCh-NDP52 in E. coli Rosetta pLysS. Internal Reference: SMC1148DepositorInsertNDP52 (CALCOCO2 Human)
Tags6xHisTag, TEV, and mCherryExpressionBacterial, Insect, and Mamm…Available SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAF92
Plasmid#135929PurposeAll-in-one Sleeping Beauty vector for Expression for Tasmanian devil mCherry-TNFSF9 (type II transmembrane) fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFSF9).DepositorInsertmCherry fused to TNFSF9
UseTransposonTagsmCherryExpressionMammalianPromoterEF1aAvailable SinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF655
Plasmid#88578PurposeDonor Vector containing ZNF655 transcription factor, part of the Human TFome CollectionDepositorInsertZNF655 (ZNF655 Human)
UseGateway donor vectorMutationLast nucleotide of stop codon removed to allow fo…Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-AMPH1-BAR-mCherry
Plasmid#85130PurposeBAR domain of human Amphiphysin1 (amino acids 1-256), C-terminally tagged with mCherryDepositorInsertBAR domain of human Amphiphysin1 (amino acids 1-256), C-terminally tagged with mCherry (AMPH Human)
TagsmCherryExpressionMammalianPromoterCAGAvailable SinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ZNF736
Plasmid#101602PurposeDonor Vector containing ZNF736 transcription factor, part of the Human TFome CollectionDepositorInsertZNF736 (ZNF736 Human)
UseGateway shuttling vectorMutationLast nucleotide of stop codon removed to allow fo…Available SinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only