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p3E-nlsGFP pA

Plasmid

#80814

Purpose

nlsGFP with pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

nlsGFPpA 3'E

Expression

Mammalian

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ymYPET-T2A-mTurquoise2

Plasmid

#118444

Purpose

Produces equimolar expression of ymYPET and mTq2

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymYPET-T2A-mTurquoise2

Expression

Yeast

Mutation

YPET A206K, F208S, E232L, N235D

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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GGE145

Plasmid

#120741

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position1 (P1_2UASxpr-TEF)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

P1_2UASxpr-TEF

Use

Cloning/assembly plasmid

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pBN237

Plasmid

#86719

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Article

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Pnhr-82::FLP D5

Expression

Worm

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pBbS18c-RFP

Plasmid

#126201

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23117

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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p3E-CMVmin:GFPmem pA

Plasmid

#80823

Purpose

CMVmin-promoted GFPmem with pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

3E-CMV-GFPCAAXpA spacer

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-oChD-citrine

Plasmid

#50976

Purpose

AAV transfer vector with mammalian codon-optimized ChD-citrine between AAV2 ITR

Depositor

Roger Tsien

Article

Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

Insert

oChD-citrine

Use

AAV

Tags

citrine

Mutation

ChD gene is mammalian codon optimized

Promoter

human synapsin promoter

Available Since

Feb. 5, 2014

Availability

Academic Institutions and Nonprofits only

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PM-TD!TA

Plasmid

#48655

Purpose

Bacterial TD crRNA expression: targets TD to protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial TD crRNA to prototspacer A

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pETMutS-E41A

Plasmid

#13048

Depositor

Peggy Hsieh

Article

Schofield et al J Biol Chem. 2001 Dec 7. 276(49):45505-8

Insert

MutS

Expression

Bacterial

Mutation

mutation Glu41 to Ala full-length

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pBbS10a-RFP

Plasmid

#126173

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23116

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pBS-NT-AscI

Plasmid

#15461

Depositor

Peter Mombaerts

Article

Walz et al J Neurobiol. 2006 Jul . 66(8):835-46.

Insert

neurotensin (Nts Mouse)

Mutation

Added AscI site 3 bp 3' of stop codon

Available Since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
PM-ST1!TB

Plasmid

#48654

Purpose

Bacterial ST1 crRNA expression: targets ST1 to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial ST1 crRNA to prototspacer B

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pBbB18k-GFP

Plasmid

#126137

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23117

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pBbB14c-GFP

Plasmid

#126129

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pBbB13c-GFP

Plasmid

#126128

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23102

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pMOR22-2-ISH

Plasmid

#15839

Depositor

Peter Mombaerts

Article

Hirota et al Mol Cell Neurosci. 2007 Apr . 34(4):679-

Insert

MOR22-2 (Olfr69 Mouse)

Use

Cloning vector

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only
pDRF1-GW ytdtomato-T2A-mTurquoise2

Plasmid

#118451

Purpose

Produces equimolar expression of yeast codon-optimized tdTomato and mTq2

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ytdtomato-T2A-mTurquoise2

Expression

Yeast

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pBbS15a-RFP

Plasmid

#126190

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23100

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pISH-Pde6d

Plasmid

#105464

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Omura et al Mol Cell Neurosci. 2015 Mar;65:114-24. d

Insert

Pde6d (Pde6d Mouse)

Use

To ganerate riboprobe

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pBN40

Plasmid

#86715

Purpose

C. elegans injection marker

Depositor

Peter Askjaer

Article

Morales-Martinez et al J Cell Sci. 2015 Mar 15;128(6):1090-6. d

Insert

Plmn-1::gfp::his-58

Expression

Worm

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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