We narrowed to 17,912 results for: por
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Plasmid#184834PurposeConstitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.DepositorInsertConstitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterJ23119Available SinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPgiCas13b-gRNA-NT
Plasmid#184835PurposeConstitutive expression of single-spacer CRISPR array with non-targeting spacer for PgiCas13b in bacteria.DepositorInsertConstitutive expression of single-spacer CRISPR array with a nontargeting spacer for PgiCas13b in bacteria.
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterJ23119Available SinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Hsp70-mplum
Plasmid#45016DepositorInsertmPlum
ExpressionMammalianPromotermouse Hspa1bAvailable SinceMay 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-Rfx4
Plasmid#61544PurposeExpresses Rfx4 in mammalian cellsDepositorAvailable SinceJan. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
tet_pLKO.1_puro_shLuc
Plasmid#136587PurposeExpresses an inducible short hairpin targeting firefly luciferase sequenceDepositorInsertshLuc
UseLentiviralExpressionMammalianAvailable SinceJune 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Caprola_04-mEGFP
Plasmid#194682PurposeCMV driven expression of the calcium recorder Caprola_04 in mammalian cell lines fused to mEGFPDepositorInsertCaprola_04-mEGFP
TagsmEGFPExpressionMammalianPromoterCMVAvailable SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GNPTAB
Plasmid#78108PurposeMammalian expression of human GNPTAB with C terminal myc tagDepositorAvailable SinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-VdC9BV
Plasmid#62195PurposeExpresses RNA-Guided, Nuclease-Inactive VP64:dCas9-BFP:VP64—VdC9BV—Fusion Protein to Enable Transactivation of Endogenous GenesDepositorInsertVP64dCas9BFPVP64
UseLentiviralTagsTwo VP64s tagged to dCas9 fused to BFPExpressionMammalianMutationD10A H840A (catalytically inactive) Cas9 (dCas9)Available SinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-Bcl-2-ΔBH4
Plasmid#220300Purposemammalian expression of Bcl-2-ΔBH4 tagged with GFPDepositorAvailable SinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Caprola_09-mEGFP
Plasmid#194685PurposeCMV driven expression of the calcium recorder Caprola_09 in mammalian cell lines fused to mEGFPDepositorInsertCaprola_09-mEGFP
TagsmEGFPExpressionMammalianPromoterCMVAvailable SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRR-ERbeta-5Z
Plasmid#23062DepositorInsertERbeta (ESR2 Human)
ExpressionYeastAvailable SinceJan. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHS0923 pET45b(+) His14-MBP-TEV-AmaTnpB
Plasmid#176587PurposeBacterial expression of His14-MBP-TEV-AmaTnpBDepositorInsertsMBP tag
TnpB
TagsHis14 tagExpressionBacterialAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-mCherry-CDMPR
Plasmid#182643PurposeMammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), followed by mCherry and murine CDMPR (without its signal peptide)DepositorInsertCDMPR
UseRetroviralTagsN-terminal ER targeting sequence of hemagglutinin…ExpressionMammalianPromoterCMVAvailable SinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-GSDMD-Myc
Plasmid#201166PurposeExpression of GSDMD gene in mammalian cells by retroviral transductionDepositorAvailable SinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cre-responsive Cas9 expressing transposon library vector
Plasmid#164138PurposeBackbone for transposon stgRNA library construction (library 4) (SpCas9 expression is induced upon Cre-loxP recombination.)DepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and Synthetic BiologyExpressionMammalianPromoterhuman U6, EF-1aAvailable SinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF-ABEmax
Plasmid#164415PurposeEF1alpha promoter + ABEmax (derived from #112095). Mammalian cell adenosine base editor expression vector.DepositorInsertABEmax
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterEF1-alphaAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEasyG2_mic
Plasmid#184916PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_mic recombines in vivo with a PCR product from pEasyG2_zeo/nat/hph.DepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFD5-U6-3-t-attP40
Plasmid#133561PurposegRNA vector for targeting near the attP40 locus, use with pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40 based constructsDepositorInsertattP40 region guide RNAs
UseCRISPRExpressionInsectPromoterU6Available SinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-Miro1 S156E
Plasmid#127589PurposePhospho-mimetic of S156DepositorAvailable SinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJK-caCas9-NatMX-Neut5L-Leu2 drive
Plasmid#89578PurposecaCas9 integrating vector into the Neut5L locus with gene drive for targeting Leu2 locusDepositorInsertLeu2 gene drive
ExpressionYeastAvailable SinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCHW90004
Plasmid#222436PurposeLevel 0, promoter module (GGAG-CCAT) containing CBS4 and tomato miniDFR promoters.DepositorInsertCBS4-miniDFR, CBS4 promoter + miniDFR minimal promoter (Solanum lycopersicum)
UseSynthetic BiologyExpressionPlantMutationnoAvailable SinceNov. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-DN-REST
Plasmid#61533PurposeExpresses DN-REST in mammalian cellsDepositorInsertREST (REST Human)
UseLentiviralExpressionMammalianMutationP73, Dominant negative REST (contains the Zf DNA-…Available SinceJan. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CamKIIa_mKate2
Plasmid#96942PurposeTo label mature excitatory iPSC-derived human neuronsDepositorInsertfar-red fluorescent protein
UseLentiviralMutationE87D (please see depositor comments below)PromoterCaMKIIaAvailable SinceAug. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
BCAM promoter-pRB-Puro-TurboRFPmyc-T2A-Luciferase
Plasmid#188391PurposeReporter for BCAM expressionDepositorInsertBCAM reporter
UseLuciferaseAvailable SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_g5-HT2h
Plasmid#208721PurposeExpresses the green 5-HT sensor GRAB_g5-HT2h in neuronsDepositorInsertGreen fluorescent 5-HT sensor GRAB_g5-HT2h
UseAAVPromoterhSynAvailable SinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
p23-NES-PguCas13b-msfGFP-NES-Flag
Plasmid#165072Purposeoverexpression of PguCas13b in human cellsDepositorInsertPguCas13b
UseLentiviralExpressionMammalianAvailable SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMCB619
Plasmid#171011PurposeCRISPR sgRNA vector (puro-BFP with BstXI/BlpI digest sites).DepositorInsertU6:sgRNA_Puro-T2A-BFP
UseCRISPR and LentiviralExpressionMammalianPromotermU6Available SinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCHW110033
Plasmid#222441PurposeLevel 0, CDS module (AATG-GCTT) containing Cre recombinase.DepositorInsertCre recombinase
UseSynthetic BiologyExpressionPlantMutationBsaI/BbsI sites removed by point-mutationAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P-1-eIF4E K119A
Plasmid#199440PurposeBacterial expression plasmids for production of recombinant GST-eIF4E K119ADepositorInsert1977 (EIF4E Human)
TagsGST-fusion proteinExpressionBacterialMutationchanged lysine 119 to alanineAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast SLC5A7
Plasmid#218539Purposehuman SLC5A7 cDNADepositorAvailable SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only