We narrowed to 159,324 results for: DES

1. pY2

   Plasmid

   #218115

   Purpose

   Activity reporter backbone with an empty protease cassette (BsaI) under (lexA-box)3PminCYC1 promoter and an empty substrate cassette (BsmBI) under pGAL1 promoter.

   Depositor

   Carl Denard

   Article

   Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pY3

   Plasmid

   #218116

   Purpose

   Activity reporter backbone with an empty protease cassette (BsaI) and an empty substrate cassette (BsmBI), both under a divergent pGAL1-10 promoter.

   Depositor

   Carl Denard

   Article

   Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pY3-LYS-IV

   Plasmid

   #218119

   Purpose

   LYS-Leu2 plasmid (218114) with the functional cassettes of pY3 (218116) inserted at the sfGFP dropout locus.

   Depositor

   Carl Denard

   Article

   Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYC34

   Plasmid

   #63915

   Purpose

   Recipient overexpressing vector to construct mCherry tagged fusions

   Depositor

   Irene Castano Navarro

   Article

   Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

   Type

   Empty backbone

   Use

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   Promoter

   TEF1 (Saccharomyces cerevisiae)

   Available since

   April 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMPMA3dPlac-PfliC-GFPLVA

   Plasmid

   #23342

   Depositor

   Olivia Steele-Mortimer

   Article

   Ibarra et al Microbiology. 2009 Dec 24. ():.

   Insert

   GFP(LVA)
   

   Use

   Tags

   GFP(LVA)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 27, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOC2 Tubb3-GFP KI

   Plasmid

   #183432

   Purpose

   CreOFF knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Insert

   gRNA and GFP donor
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-deltaR8.2(A14C,E45C)

   Plasmid

   #79047

   Purpose

   mammalian expression, lentiviral packaging

   Depositor

   Wesley Sundquist

   Article

   Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. Ai38

   Plasmid

   #34883

   Depositor

   Hongkui Zeng

   Article

   Zariwala et al J Neurosci. 2012 Feb 29;32(9):3131-41. d

   Insert

   CAG-Floxed GCaMP3
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   March 7, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFLAG-4EHP

   Plasmid

   #17342

   Depositor

   Dong-Er Zhang

   Article

   Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

   Insert

   4EHP (EIF4E2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

10. Ubiquitin I44A

    Plasmid

    #12648

    Depositor

    Rachel Klevit

    Article

    Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

    Insert

    Ubiquitin (UBC Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Ile44Ala

    Promoter

    Available since

    July 31, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pFA6a-link-ymVenus-URA3

    Plasmid

    #168058

    Purpose

    Replaces Addgene plasmid 118459. Yeast tagging vector to create a gene fusion with yeast optimized mVenus with CaUra3 selection; Contains the linker region at the 5’ end of the FP

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymVenus
    

    Use

    Tags

    ymVenus

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOC1 Gria1-GFP-FRB KI

    Plasmid

    #183428

    Purpose

    CreOFF knock-in for GluA1-GFP-FRB (amino acid position: stop codon)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and GFP-FRB donor
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOC2 Dlg4-Halo-FKBP KI

    Plasmid

    #183435

    Purpose

    CreOFF knock-in for PSD95-Halo-FKBP (amino acid position: R721)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and Halo-FKBP donor
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAIO-LbCpf1

    Plasmid

    #137847

    Purpose

    LbCpf1 and gRNA expression plasmid for P. falciparum; no selection in parasites.

    Depositor

    Josh Beck

    Article

    Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

    Insert

    LbCpf1-NLS-FLAG
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOC3 smFP-HA-Cacna1e KI

    Plasmid

    #183436

    Purpose

    CreON FlpOFF knock-in for smFP-HA-CaV2.3 (amino acid position: G5)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and smFP HA donor
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCTCON2-GFP-TAG

    Plasmid

    #158131

    Purpose

    Single fluorescent protein reporter (GFP) for ncAA incorporation (TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Introduced a stop codon in place of the tyrosine …

    Promoter

    GAL1-10

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYC46

    Plasmid

    #63904

    Purpose

    Recipient integrative vector to construct FLAG tagged fusions

    Depositor

    Irene Castano Navarro

    Article

    Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

    Type

    Empty backbone

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEGFP-C1-hRabin8 F201A

    Plasmid

    #26728

    Depositor

    Keith Mostov

    Article

    Bryant et al Nat Cell Biol. 2010 Oct 3. ():.

    Insert

    Rabin8 (RAB3IP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    F201A: GEF domain mutant

    Promoter

    Available since

    Dec. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pYC61

    Plasmid

    #63925

    Purpose

    Recipient replicative vector to construct CFP tagged fusions

    Depositor

    Irene Castano Navarro

    Article

    Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

    Type

    Empty backbone

    Use

    Tags

    CFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYC55

    Plasmid

    #63922

    Purpose

    Recipient replicative vector to construct YFP tagged fusions

    Depositor

    Irene Castano Navarro

    Article

    Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

    Type

    Empty backbone

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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