We narrowed to 68,316 results for: cha
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Plasmid#60488Purposegag/pol packaging plasmid for production of lentiviral vectors with mutated reverse transcriptase (NRTLV, non-reverse transcribable lentiviral vectors)DepositorInsertHIV-1 gag, HIV-1 pol with inactive reverse transcriptase
Mutationchanged Asp at position 249 + 250 to ValAvailable SinceOct. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-Tight-MCS_PGK-GpNLuc
Plasmid#70185PurposeConstiutive mammalian expression of the bioluminescent GpNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.DepositorTypeEmpty backboneUseRetroviralExpressionMammalianPromoterPGKAvailable SinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaTdT-39
Plasmid#228980PurposeFor bacterial expression of anti-tdTomato nanobody LaTdT-39, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertLaTdT-39
Tags6xHISExpressionBacterialPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pWR76
Plasmid#202776Purposeepisomal fluorescent reporter plasmid for S. stipitisDepositorInsertsTEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCherry
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1
ExpressionYeastAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-Tight-MCS_PGK-OgNLuc
Plasmid#70186PurposeConstiutive mammalian expression of the bioluminescent OgNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.DepositorTypeEmpty backboneUseRetroviralExpressionMammalianPromoterPGKAvailable SinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTol2 hsp70l loxP-Non-FP-loxP-nCas9n-GFP
Plasmid#158963PurposeCre-dependet Cas9-GFP expressionDepositorInsertshsp70l promoter
floxed Non-FP cassette
Cas9-GFP
UseCRISPR and Cre/LoxAvailable SinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS-MXT GIRK2
Plasmid#20675DepositorInsertMus musculus G protien coupled inward rectifier K+ channel 2 (Kcnj6 Mouse)
UseXenopus expressionTags3' UTR Xenopus B- globin and 5' UTR Xen…MutationA369VAvailable SinceMarch 31, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-mRuby2
Plasmid#99126PurposeAn AAV genome that expresses the fluorescent protein mRuby2 from the hSyn1 promoterDepositorInsertmRuby2
UseAAVExpressionMammalianPromoterhSyn1Available SinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-MC38-TCRA-33-MC_hammer
Plasmid#138989PurposeIVT template for the alpha subunit of the mouse TCR #33 that is reactive against MC38DepositorAvailable SinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-6
Plasmid#172771PurposeBacterial expression of anti-mCherry nanobody LaM-6, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-6
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFsynW SYT1 reporter
Plasmid#197275PurposeEncodes for viral expression of wild type Synaptotagmin 1 (SYT1) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).DepositorInsertSYT1 and HaloTag (Syt1 Rat)
UseLentiviralTagsFLAGMutationN/APromoterhSyn (human synapsin I promoter)Available SinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
UPB-colon (UPB4)
Plasmid#180786PurposeUnique Projection Barcode (UPB4)-Colon for Projection-seqDepositorInserthM3Dq ( partial )
UseAAVExpressionMammalianPromoterCAGAvailable SinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
piggyFlex
Plasmid#218234PurposeA piggyBac transposon-based gRNA expression vector, to allow for genomic integration and stable expression of gRNAs. Contains both antibiotic (puromycin) and fluorophore (GFP) markers.DepositorTypeEmpty backboneUseCRISPRAvailable SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
SPOP-V5 (F133V)
Plasmid#169725PurposeMammalian Expression of mouse SPOP F133VDepositorAvailable SinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn SYP-HaloTag
Plasmid#188907PurposeExpresses synaptic HaloTagDepositorAvailable SinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEC3006 (pSpy1C-PZn_ffluc)
Plasmid#218515Purposereplicative E. coli-S. pyogenes shuttle plasmid for zinc-inducible expression of the Firefly luciferase in S. pyogenesDepositorInsertffluc
UseLuciferaseExpressionBacterialMutationdeletion of Plac_mrfp cassettePromoterPZn (PczcD)Available SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
SPOP-V5 (W131G)
Plasmid#169724PurposeMammalian Expression of mouse SPOP W131GDepositorAvailable SinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET15b SARS-CoV PLpro
Plasmid#61693Purposeinducible expression of SARS PLpro proteaseDepositorInsertSARS-CoV PLpro
TagsHisExpressionBacterialPromoterT7Available SinceApril 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pmGFP-Sec16S
Plasmid#15775DepositorAvailable SinceNov. 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
EKARdual
Plasmid#99870PurposeMeasure ERK activity by conformational change after ERK substrate phosphorylation and recognition of this phosphorylated substrateDepositorInsertEKAR
TagsShadowG and mTFP1ExpressionMammalianPromoterCMVAvailable SinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ChRger2-TS-EYFP
Plasmid#127237PurposeHigh photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.DepositorInsertChRger2-TS-EYFP
UseAAVTagsEYFP and SpyTagExpressionMammalianPromoterhSyn1Available SinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGMCtKq28-TSC10M1-sspB
Plasmid#49526PurposeKanamycin resistant; contains qtag28, expresses sspB under a promoter containing PtetO, and constutively expresses single-chain TetR10 (tsc10); integrates into the mycobacteriophage att-Tweety siteDepositorInsertTSC10M1-sspB
ExpressionBacterialAvailable SinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-GST-2
Plasmid#228984PurposeFor bacterial expression of anti-GST nanobody GST-2, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertGST-2
Tags6xHISExpressionBacterialPromoterT7Available SinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-GST-1
Plasmid#228983PurposeFor bacterial expression of anti-GST nanobody GST-1, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertGST-1
Tags6xHISExpressionBacterialPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Camk2b
Plasmid#186819PurposeFluorescent reporter for Camk2b expressionDepositorAvailable SinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Camk2d
Plasmid#186820PurposeFluorescent reporter for Camk2d expressionDepositorAvailable SinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGl3-Camk2g
Plasmid#186821PurposeFluorescent reporter for Camk2g expressionDepositorAvailable SinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
FAB(ER-LD)
Plasmid#214424PurposeDetection of ER-lipid droplet contact siteDepositorInsertFRB-V5
TagsCFAST-Halo and RspAN-FRB-V5ExpressionMammalianPromoterCMVAvailable SinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL1-sfGFP-DQNAT
Plasmid#198210PurposepJL1 plasmid encoding superfolder GFP modified at the C-terminus with 30 amino acids containing an optimal DQNAT sequon followed by a 6x-His tagDepositorInsertsfGFP_DQNAT
Tags6x His Tag and DQNAT glycosylation tagExpressionBacterialPromoterT7Available SinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-Ago1
Plasmid#21534DepositorAvailable SinceJuly 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
Lenti-mApple-6xHp
Plasmid#217376PurposeLipid droplet localization of 6xHp of Spastin fused to mAppleDepositorInsertmApple-6x Hp NC
UseLentiviralTagsmApplePromoterCMVAvailable SinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB115
Plasmid#68569PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsNuclear localization signal-tagged Citrine (Citri…ExpressionPlantAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-omega
Plasmid#114632PurposeAddgene plasmid 44249 where dCas9 has been replaced with dCas9 fused to the omega subunit of RNAPDepositorInsertdCas9-omega
UseCRISPRExpressionBacterialPromoterTet promoterAvailable SinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
SPOP-V5 (BACK))
Plasmid#169722PurposeMammalian Expression of mouse SPOP Y353EDepositorAvailable SinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-mNeonGreen
Plasmid#99132PurposeAn AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein mNeonGreenDepositorInsertDIO-mNeonGreen
UseAAVExpressionMammalianPromoterTREAvailable SinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJEC726
Plasmid#190814PurposeCRISPRi backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.DepositorInsertdCas9
UseCRISPR and Synthetic BiologyMutationS. pyogenes dCas9 codon optimized for Streptomyce…PromoterSP30Available SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC-6-GFP-P2A-NptII-9
Plasmid#197741PurposePhytobrick (MoClo) Level 0 PartDepositorInsertEGFP-P2A-NptII bicistronic CDS
ExpressionPlantAvailable SinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_VPR_EBFP2
Plasmid#167881PurposePiggyBac compatible plasmid expressing dCas9-VPRDepositorInsertdCas9-VPR
UseCRISPRMutationD10A, D839A, H840A, N863AAvailable SinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-hXRCC1
Plasmid#52274PurposeBacterial expression of human XRCC1 with N-terminal GST-tagDepositorAvailable SinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
EYFP-Golgi-7
Plasmid#56590PurposeLocalization: Golgi, Excitation: 513, Emission: 527DepositorInsertGolgi (B4GALT1 Human)
TagsEYFPExpressionMammalianMutationaa 1-82 of NM_001497.3PromoterCMVAvailable SinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits