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We narrowed to 13,844 results for: cas9 genes

Showing: 9921 - 9960 of 13844 results

  1. pCG-scr

    Plasmid
    #229847
    Purpose
    Expresses wild-type Cas9 and scrambled gRNA.
    Depositor
    Natsuko Chiba
    Insert
    guide RNA with scrambled sequence
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    229847-plasmid-map-sequence-id-457515

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  2. pSBbi-RB-PE2

    Plasmid
    #173902
    Purpose
    SB-transposon with constitutive expression of SpCas9 PE2
    Depositor
    Ronald Cohn
    Insert
    PE2
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    173902-plasmid-map-sequence-id-346342

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  3. pCAMTA2.1.0-gDNA

    Plasmid
    #132456
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    CAMTA2 (CAMTA2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    132456-plasmid-map-sequence-id-266911

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  4. pMBD1.1.0-gDNA

    Plasmid
    #132444
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    MBD1 (MBD1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    132444-plasmid-map-sequence-id-266804

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  5. pML104-KanMx4

    Plasmid
    #83476
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 marker for yeast transformation.
    Depositor
    Patricia Wittkopp
    Insert
    KanMX4
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    83476-plasmid-map-sequence-id-189195

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  6. pML104-HygMx4

    Plasmid
    #83475
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains HphMx4 marker for yeast transformation.
    Depositor
    Patricia Wittkopp
    Insert
    HphMX4
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    83475-plasmid-map-sequence-id-358352

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  7. pET-MBP-NLS-Geo_st

    Plasmid
    #87703
    Purpose
    Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP and SV40 NLS
    Depositor
    Jennifer Doudna
    Insert
    GeoCas9
    Use
    Tags
    10xHis-MBP-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87703-plasmid-map-sequence-id-176992

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  8. pML104-NatMx3

    Plasmid
    #83477
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains NatMX3 marker for yeast transformation.
    Depositor
    Patricia Wittkopp
    Insert
    NatMx3
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only

  9. HCP1

    Plasmid
    #166103
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination.
    Depositor
    Matthias Heinemann
    Insert
    Msn2-sgRNA#27 (MSN2 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166103-plasmid-map-sequence-id-330378

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  10. AICSDP-49: MYL2-mEGFP

    Plasmid
    #114414
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human MYL2, via Cas9-excisable CAGGS-mCherry selection cassette
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Stem Cell Reports. 2019 May 14;12(5):114
    Insert
    MYL2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (MYL2 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt ā€¦
    Promoter
    Available since
    Sept. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    114414-plasmid-map-sequence-id-220893

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  11. AICSDP-46: MYL7-mEGFP

    Plasmid
    #114413
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human MYL7, via Cas9-excisable CAGGS-mCherry selection cassette
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Stem Cell Reports. 2019 May 14;12(5):114
    Insert
    MYL7 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (MYL7 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt ā€¦
    Promoter
    Available since
    Sept. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    114413-plasmid-map-sequence-id-250980

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  12. Pdpy-30-miniCAFE

    Plasmid
    #170115
    Purpose
    Dpy-30 promoter-driven expression of a truncated VPR-dCjCas9 fusion protein (MiniCAFE) for activation of gene expression.
    Depositor
    Zhili Rong
    Article
    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617
    Insert
    MiniCAFE
    Use
    CRISPR
    Tags
    Expression
    Mutation
    D8A, HNH-truncation (Ī”495ā€“609 aa) in CjCas9
    Promoter
    Dpy-30
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    170115-plasmid-map-sequence-id-336055

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  13. AICSDP-7:SEC61B-mEGFP

    Plasmid
    #87426
    Purpose
    Homology arms and linker-mEGFP sequence for N-terminus tagging of human SEC61B
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Insert
    SEC61B Homology Arms with mEGFP-linker (SEC61B Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt ā€¦
    Promoter
    Available since
    March 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87426-plasmid-map-sequence-id-173967

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  14. AICSDP-10:LMNB1-mEGFP

    Plasmid
    #87422
    Purpose
    Homology arms and linker-mEGFP sequence for N-terminus tagging of human LMNB1
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Insert
    LMNB1 Homology Arms with mEGFP-linker (LMNB1 Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt ā€¦
    Promoter
    Available since
    March 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87422-plasmid-map-sequence-id-173949

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  15. AICSDP-8:TOMM20-mEGFP

    Plasmid
    #87423
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human TOMM20
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Insert
    TOMM20 Homology Arms with linker-mEGFP (TOMM20 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt ā€¦
    Promoter
    Available since
    March 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87423-plasmid-map-sequence-id-173982

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  16. AICSDP-4:TUBA1B-mEGFP

    Plasmid
    #87421
    Purpose
    Homology arms and linker-mEGFP sequence for N-terminus (second exon) tagging of human TUBA1B
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Insert
    TUBA1B Homology Arms with mEGFP-linker (TUBA1B Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt ā€¦
    Promoter
    Available since
    March 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87421-plasmid-map-sequence-id-441601

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  17. AICSDP-9:DSP-mEGFP

    Plasmid
    #87424
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human DSP
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Insert
    DSP Homology Arms with linker-mEGFP (DSP Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt ā€¦
    Promoter
    Available since
    March 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87424-plasmid-map-sequence-id-173979

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  18. AICSDP-13:FBL-mEGFP

    Plasmid
    #87427
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human FBL
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Insert
    FBL Homology Arms with linker-mEGFP (FBL Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt ā€¦
    Promoter
    Available since
    March 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87427-plasmid-map-sequence-id-173980

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  19. pYUU

    Plasmid
    #159751
    Purpose
    Ubiquitin promoter-controlled expression of 3ƗFLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by YFP fluoresce.
    Depositor
    Wolfgang Lukowitz
    Article
    Angulo et al bioRxiv
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159751-plasmid-map-sequence-id-313821

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  20. B2M Bulldozer (gRNA crB2M_13)

    Plasmid
    #84381
    Purpose
    This plasmid expresses a gRNA targeting the first exon of human B2M. Most efficient gRNA targeting B2M, leading to ablation of B2M and MHC class I surface expression in 50% of transfected cells
    Depositor
    Chad Cowan
    Article
    Mandal et al Cell Stem Cell. 2014 Nov 6;15(5):643-52.
    Insert
    B2M Bulldozer crB2M_13 gRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6 promoter
    Available since
    July 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    84381-plasmid-map-sequence-id-189203

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  21. pTUU

    Plasmid
    #159749
    Purpose
    Ubiquitin promoter-controlled expression of 3ƗFLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by mTomato fluoresce.
    Depositor
    Wolfgang Lukowitz
    Article
    Angulo et al bioRxiv
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159749-plasmid-map-sequence-id-313818

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  23. pCUU

    Plasmid
    #159747
    Purpose
    Ubiquitin promoter-controlled expression of 3ƗFLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by CFP fluoresce.
    Depositor
    Wolfgang Lukowitz
    Article
    Angulo et al bioRxiv
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159747-plasmid-map-sequence-id-313815

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  27. plenti-Lb-Cas12a-2xNLS

    Plasmid
    #155046
    Purpose
    Lentiviral vector expressing human codon-optimized _C-terminal Myc-tagged Lachnospiraceae bacterium (Lb)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoter
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    LbCas12a
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS, Myc-tag and SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Aug. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    155046-plasmid-map-sequence-id-308670

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  34. PL-5LTR-GW-A

    Plasmid
    #138480
    Purpose
    For expression of ribozyme-flanked sgRNA after the Gateway (GW) LR cloning.
    Depositor
    Akitsu Hotta
    Article
    Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:
    Insert
    Gateway attR1/R2 cassette with chloramphenicol resistance and ccdB genes
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    138480-plasmid-map-sequence-id-275574

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  40. AAV-CMV-scFVDnmt3AL_bGHpA

    Plasmid
    #177347
    Purpose
    AAV expression of a fusion protein with scFV, catalytic domain of Dnmt3a and C terminal domain of Dnmt3l for targeted DNA methylation editing
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Insert
    catalytic domain of DNA Methyltransferase 3 Alpha (Dnmt3a Mouse)
    Use
    AAV
    Tags
    C-terminal domain of mouse Dnmt3l (194ā€“415 aa), Pā€¦
    Expression
    Mammalian
    Mutation
    598ā€“908 aa of mouse Dnmt3a
    Promoter
    CMV
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177347-plasmid-map-sequence-id-353618

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Showing: 9921 - 9960 of 13844 results