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Plasmid#204230PurposeBacterial expression of mitochondrial human malate dehydrogenase 2 (hMDH2) transcript variant 1; Use in CURES (integration of research into undergraduate teaching labs).InserthMDH2 (MDH2 Human)
UseTags6X HisExpressionBacterialMutationDoes not include transit peptide and includes a M…PromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pYES2.1-pomSir1
Plasmid#107447PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertSir1 (sir1 Fission Yeast)
UseTagsV5-HisExpressionYeastMutationH523R, A750TPromoterGAL1Available sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
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C-SWAT IAA17-6FLAG (no NLS)
Plasmid#193322PurposeC-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin-Inducible Degron) and 6FLAG. ARF16(PB1)-P2A-osTIR1 has SIR4 homology arms for genome integration and does not have NLS tags..DepositorInsertIAA17
UseTags6FLAGExpressionYeastMutationPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT GFP CL1 DAmP
Plasmid#182513PurposeC-SWAT compatible vector for C-terminal tagging with GFP CL1 followed by the DAmP cassette. The stop codon after the CL1 degron is immediately followed by the Hygromycin B (Hph) resistance cassette.DepositorInsertCL1 and DAmP
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJUMP18-RBS-pET-MV_RN
Plasmid#127014PurposeBasic Part RN- RBS+N-terminus; B0032m Ribosome Binding Site + Met-Val N-terminus start.DepositorInsertPart RBS-pET-MV_RN
UseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-MAVS-M1A500(KaganF60)
Plasmid#52003PurposeExpresses the human MAVS CDS containing a point mutation M1A and a C-terminal deletion removing the transmembrane domainDepositorInsertMAVS-M1A-500
UseTagsExpressionMammalianMutationchanged Met 1 to Ala and deleted the C-terminal t…PromoterAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVP91A-pcaHG
Plasmid#113766PurposeExpresses the alpha (pcaG) and the beta (pcaH) subunits of Protocatechuate 3,4-Dioxygenase from Pseudomonas putida.DepositorInsertsβ subunit of Protocatechuate 3,4- Dioxygenase
α subunit of Protocatechuate 3,4- Dioxygenase
UseTagsMet-6xHisExpressionBacterialMutationPromoterT5 promoterAvailable sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
TrpLE-M-nMSP1E3D1
Plasmid#179949PurposeTagless membrane scaffold protein (MSP) for formation of nanodiscs.DepositorInsertTrpLE-M-nMSP1E3D1
UseTagsTrpLEExpressionBacterialMutationNative Trp residues have been mutated to Phe resi…PromoterT7Available sinceFeb. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET11a-4SS-HEWL
Plasmid#39233DepositorInsertHen egg white lysozyme (LYZ Chicken)
UseTagsExpressionBacterialMutationN-terminal MetPromoterT7Available sinceSept. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
SG3ΔENV CA M66I
Plasmid#149687PurposeNon-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and M66I mutation in capsid (CA M66I).DepositorInsertCapsid
UseLentiviral and RetroviralTagsExpressionMammalianMutationChanged Capsid residue Met 66 to Iso (CA M66I)PromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET23-PBD(65-150)-N-Cys
Plasmid#13722DepositorInsertPBD(65-150)-N-Cys (PAK1 Human)
UseTags6xHisExpressionBacterialMutationMet-Cys-Gly-PBD(65-150)-PromoterAvailable sinceMarch 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGCNK58
Plasmid#202461PurposePlasmid for the expression of the basic region and leucine zipper domain of the Saccharomyces cerevisiae transcription factor GCN4 in bacterial cellsDepositorInsertResidues 226-281 of Sacchromyces cerevisiae GCN4 plus an N-terminal Met-Lys extension (GCN4 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pTriEx4-H6-FGAm (FlincG3)
Plasmid#49202PurposeFluorescent reporter for cGMPDepositorInsertFlincG3
UseTagsHis tag + other tagsExpressionBacterial, Insect, and Mamm…MutationMet to Lys in cpEGFP region similar to CGaMP3PromoterCMVAvailable sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_LAMA-F98 (for GFP)
Plasmid#130714PurposePlasmid encoding the bacterial expression of LAMA-F98 for GFPDepositorInsertLAMA-F98
UseTagsHisx10ExpressionBacterialMutationDeleted first Met, Ala and Last Ser from the Enha…PromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV/N3IC-HA
Plasmid#47618Purposein vitro transcription and translation of HA-tagged Notch3 Intracellular domainDepositorInsertNotch3 Intracellular domain (Notch3 Mouse)
UseIn vitro transcription and translationTagsHAExpressionMammalianMutationcodons 1664 (Met) to 2318 (Ala)PromoterCMVAvailable sinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only