We narrowed to 17,429 results for: She

1. pDEST-FRT-TO-GFP-PALB2 KR

   Plasmid

   #71107

   Purpose

   mammalian expression of GFP-PALB2 mutant. (first 8 Lysine residues mutated to Arginine)

   Depositor

   Daniel Durocher

   Article

   Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

   Insert

   PALB2 (PALB2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   first 8 Lysine residues mutated to Arginine (K14-…

   Promoter

   CMV/TO

   Available since

   Dec. 2, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pRK5 CASTOR2-HA Cterm

   Plasmid

   #84489

   Purpose

   overexpression of CASTOR2 with HA tag

   Depositor

   David Sabatini

   Article

   Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

   Insert

   CASTOR2 (GATSL2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pEPQD2KN0897

   Plasmid

   #177090

   Purpose

   MoClo Level 2 plasmid containing multiple transcriptional units for transient expression of CrISY, NmMLPL, Cr7-DLGT, CrGOR, CrIO, and CrG8H driven by 4x opaatB

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   CrISY, NmMLPL, Cr7-DLGT, CrGOR, CrIO, and CrG8H
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-TAT-2-130-FLAG

   Plasmid

   #35984

   Depositor

   Susana Valente

   Article

   Mousseau et al Cell Host Microbe. 2012 Jul 19;12(1):97-

   Insert

   transactivating protein (tat )
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   tat from HIV2 130 amino acids

   Promoter

   Available since

   Sept. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKD280.7

   Plasmid

   #173172

   Purpose

   Expresses SO_4387

   Depositor

   Jeffrey Tabor

   Article

   Cartwright et al Proc Natl Acad Sci U S A. 2021 May 18;11

   Insert

   SO_4387
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23107

   Available since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Halo-Tev-Akt3 WT-2X Flag

   Plasmid

   #87607

   Purpose

   expresses halo tagged at the N-terminus and 2 tandem flag tags at the C-terminus of human Akt3 protein

   Depositor

   Yimon Aye

   Insert

   Akt3 isoform 2 (AKT3 Human)
   

   Use

   Zebrafish (danio rerio) expression

   Tags

   2 tandem flag tags and His-Halo-Tev

   Expression

   Mammalian

   Mutation

   Akt3 isoform 2

   Promoter

   SP6

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLHCX-Flag-mPKM2(K433E)

   Plasmid

   #42514

   Depositor

   Dimitrios Anastasiou

   Article

   Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

   Insert

   Pyruvate Kinase M2 Lys433->Glu
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mutation

   Changed lysine 433 to glutamate

   Promoter

   Available since

   March 19, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pEGFP-C-SLC35B2

   Plasmid

   #155037

   Purpose

   Transient expression of EGFP-SLC35B2

   Depositor

   Yihong Ye

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

   Insert

   SLC35B2 (SLC35B2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. POT4-RFP

   Plasmid

   #65320

   Purpose

   receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

   Depositor

   Junbiao Dai

   Article

   Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

   Insert

   RFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. ELC-Fc

    Plasmid

    #8636

    Depositor

    Timothy Springer

    Article

    Manjunath et al J Clin Invest 2001 Sep;108(6):871-8.

    Insert

    CCL19 (CCL19 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 23, 2005

    Availability

    Academic Institutions and Nonprofits only

11. pMV-LEU2

    Plasmid

    #65334

    Purpose

    standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker LEU2 which could be released and ligated to series of TUs

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    LEU2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-NLS-FRB-VP16

    Plasmid

    #210515

    Purpose

    expresses NLS-FRB-VP16 component in mammalian cells

    Depositor

    Dongmin Lee

    Article

    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

    Insert

    NLS-FRB-VP16
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTYF-sGFAP-LOx-IRES-tdTomato

    Plasmid

    #166919

    Purpose

    Express the bacterial lactate oxidase (LOx) enzyme in astrocytes

    Depositor

    Sergey Kasparov

    Article

    Vaccari Cardoso et al Glia. 2021 Jan 5. doi: 10.1002/glia.2396

    Insert

    Lactate oxidase
    

    Use

    Lentiviral

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    sGFAP

    Available since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLA CMV N-Flag RALB WT

    Plasmid

    #50990

    Purpose

    Expresses RALB in mammalian cells

    Depositor

    Anna Sablina

    Article

    Simicek et al Nat Cell Biol. 2013 Oct;15(10):1220-30.

    Insert

    RALB (RALB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

15. GWF180

    Plasmid

    #133607

    Purpose

    CAG-DNCR2-VPR-IRES-Puro-WPRE-SV40PA-PGK-NS3aH1-tagBFP-SpdCas9

    Depositor

    Dustin Maly

    Article

    Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

    Insert

    DNCR2-VPR, NS3aH1-tagBFP-SpdCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. OVA-miSFIT-8-G

    Plasmid

    #124675

    Purpose

    Tuning expression of cytosolic Ovalbumin coupled to EGFP

    Depositor

    Tudor Fulga

    Article

    Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

    Insert

    Cyto-Ovalbumin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Truncated OVA for cytoplasmic expression

    Promoter

    minimal CMV

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-CAG-miniSOG-T2A-dTomato

    Plasmid

    #193796

    Purpose

    Expresses miniSOG in the cytosol of mammalian cells, joined by a T2A cleavage site with dTomato

    Depositor

    Xiao Wang

    Article

    Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

    Insert

    miniSOG
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAV-SYN-eGFP-RAB11

    Plasmid

    #203731

    Purpose

    AAV vector plasmid expressing human RAB11 fused to eGFP under the human synapsin (SYN) promoter

    Depositor

    Bart Nieuwenhuis, Richard Eva

    Insert

    RAB11 (RAB11A Human)
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Human synapsin promoter

    Available since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPB-CAG-Flag3-pG-mNanog-pgk-hph

    Plasmid

    #74911

    Purpose

    Mammalian expression of Flag tagged mNanog

    Depositor

    Jose Silva

    Article

    Theunissen et al Development. 2011 Nov;138(22):4853-65. d

    Insert

    Nanog (Nanog Mouse)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PKC-delta-IRES-hrGFP

    Plasmid

    #73414

    Purpose

    Mammalian expression of mouse PKC-delta and hrGFP

    Depositor

    Raymond Swanson

    Article

    Brennan-Minnella et al Cell Death Dis. 2013 Apr 4;4:e580. doi:

    Insert

    PKC-delta (Prkcd Mouse)
    

    Use

    Tags

    3xFLAG and IRES-hrGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

21. pLL3.7-hSt3Cas9 (pXK-1721)

    Plasmid

    #208828

    Purpose

    CRISPR/hSt3Cas9 expression vector for Animal gene editing, harboring the humanized St3Cas9 gene and the corresponding optimized SP1.gRNA. Key Construct: mU6- SP1.gRNA-CMV-hSt3Cas9.

    Depositor

    Kun Xu

    Article

    Xu et al Cell Mol Life Sci. 2015 Jan;72(2):383-99

    Type

    Empty backbone

    Use

    CRISPR; Stcas9

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGL3-24p3/LCN2-282-LUC

    Plasmid

    #46865

    Purpose

    murine 24p3 minimal promoter as a luciferase reporter

    Depositor

    Sarah Gaffen

    Article

    Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    24p3/lipocalin 2 proximal promoter
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    24p3 minimal promoter

    Available since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

23. pMtb CblA

    Plasmid

    #138298

    Purpose

    Expression of Mtb CblA

    Depositor

    Ruma Banerjee

    Article

    Ruetz et al Science. 2019 Nov 1;366(6465):589-593. d

    Insert

    Mtb CblA
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMtb ATR

    Plasmid

    #138297

    Purpose

    Expression of Mtb ATR

    Depositor

    Ruma Banerjee

    Article

    Ruetz et al Science. 2019 Nov 1;366(6465):589-593. d

    Insert

    Corrinoid adenosyl transferase
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pKD280

    Plasmid

    #125071

    Purpose

    Expression of SO_4387 under Ptac

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    SO_4387 (SO_4387 )
    lacI (lacI )
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    S286L- Please see depositor comments below

    Promoter

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1-P2X2-CFP

    Plasmid

    #22401

    Depositor

    Henry Lester

    Article

    Khakh et al J Neurosci. 2005 Jul 20. 25(29):6911-20.

    Insert

    ATP-gated P2X channel 2 (P2rx2 Rat)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

27. pT2K-CAGGS-U6-sgRNA-M5-U6-sgRNA-M7-U6-sgRNA-M9-IRES-CFP

    Plasmid

    #114729

    Purpose

    3 sgRNAs targeting a sequence upstream of the initiator ATG of the cellular Myc gene

    Depositor

    Martine Roussel

    Article

    Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

    Insert

    sgRNA M5, sgRNA M7, sgRNA M9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBabe_puro_DEST_Flag_SLC2A2

    Plasmid

    #45267

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    SLC2A2 (SLC2A2 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

29. HSD17B10

    Plasmid

    #39153

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    HSD17B10 (HSD17B10 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    codon-optimized

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

30. pet16b ALIX Bro1 (1-359)

    Plasmid

    #80641

    Purpose

    Residues 1-359 of ALIX; Bacterial Expression Vector; N-term His-tag; Sundquist Lab Internal ID: WISP11-428

    Depositor

    Wesley Sundquist

    Article

    McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

    Insert

    ALIX Bro1, residues 1-359 (PDCD6IP Human)
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Residues 1-359

    Promoter

    T7

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. boP58-pBabe.puro(notag)

    Plasmid

    #21885

    Depositor

    David Ron

    Article

    Oyadomari et al Cell. 2006 Aug 25. 126(4):727-39.

    Insert

    P58 (DNAJC3 )
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pSIN-Pdha1

    Plasmid

    #175936

    Purpose

    knockout mouse Pdha1

    Depositor

    Dan Littman

    Article

    Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

    Insert

    Pdha1 gRNA (Pdha1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pJL1-EPA-DNNNS-DQNRT

    Plasmid

    #128397

    Purpose

    In vitro expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Exotoxin A
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    242DNNNS246 and 384DQNRT388 engineered glycosylat…

    Promoter

    T7

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAAV-pEF1a-DIO-tTAc

    Plasmid

    #172126

    Purpose

    Expression of InteinC-tTAC in Cre recombinase positive cells

    Depositor

    Chun Xu

    Article

    Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

    Insert

    InteinC-tTAc
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CaMKII

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAV-EF1a-FRT-EBFP-tTAn

    Plasmid

    #172127

    Purpose

    Expression of EBFP and tTA-InteinN in Flp recombinase positive cells

    Depositor

    Chun Xu

    Article

    Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

    Insert

    EBFP, tTAn-InteinN
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. AAV-SYN-integrin alpha9-V5

    Plasmid

    #203729

    Purpose

    AAV vector plasmid expressing human integrin subunit alpha 9 (ITGA9) fused to V5-tag under the human synapsin (SYN) promoter

    Depositor

    Bart Nieuwenhuis, Richard Eva

    Insert

    ITGA9 (ITGA9 Human)
    

    Use

    AAV

    Tags

    V5-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Human synapsin promoter

    Available since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET151-Vps4(101-437)

    Plasmid

    #87735

    Purpose

    Bacterial expression of N-terminally truncated S. cerevisiae Vps4

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Use

    Tags

    6xHis-V5-TEV

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-100

    Promoter

    T7

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET-SOMA

    Plasmid

    #118935

    Purpose

    Used to express the SOMA FRET sensor in E. coli.

    Depositor

    Patrick Krysan

    Article

    Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

    Insert

    SOMA FRET sensor for measuring MAPK activity in Arabidopsis thaliana
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. gRNA-HEK3-Puro

    Plasmid

    #136282

    Purpose

    Expresses a guide RNA targeting HEK3 site

    Depositor

    Zhaohui Ye

    Article

    McGrath et al Nat Commun. 2019 Nov 25;10(1):5353. doi:

    Insert

    sgRNA-HEK3
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCMV4-Flag-mIL-17RA/YFP.Hygro

    Plasmid

    #46862

    Purpose

    encodes Flag-tagged mouse IL-17RA fused to Yellow Fl. Protein

    Depositor

    Sarah Gaffen

    Article

    Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    IL-17RA-YFP
    

    Use

    Tags

    Flag/IL-3 signal sequence and fused to CFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only