We narrowed to 23,648 results for: crispr

1. pCW-Tet-Cas9-BFP-PGK-Blast

   Plasmid

   #196720

   Purpose

   Tet-inducible expression of Cas9-T2A-TagBFP

   Depositor

   Bernhard Schmierer

   Insert

   Cas9-T2A-TagBFP
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG-SV40NLS and Nucleoplasmin NLS

   Promoter

   TRE, PGK

   Available Since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. CROPseq-Guide-EFS-SpCas9-P2A-EGFP

   Plasmid

   #99248

   Purpose

   single-vector system with EGFP reporter for use in CROPseq knockout screens

   Depositor

   Alex Hewitt

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Nov. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSaCas9_GFP

   Plasmid

   #64709

   Purpose

   Co-expression of human codon-optimized Staphylococcus aureus Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells and other mammalian cells

   Depositor

   Kiran Musunuru

   Insert

   SaCas9-2A-GFP
   

   Use

   CRISPR

   Tags

   2A-GFP and 3xFLAG-SV40 NLS

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. 1782_pAAV-U6-Ai9-Sa-gRNA2-CB-SACas9-HA-OLLAS-spA_C

   Plasmid

   #135978

   Purpose

   gRNA against the right loxP site of the Ai9 allele

   Depositor

   William Lagor

   Insert

   Ai9 gRNA2
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   HA

   Promoter

   CB

   Available Since

   Jan. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-ABE-P48R

   Plasmid

   #161816

   Purpose

   Expresses ABE-P48R in mammalian cells

   Depositor

   Sangsu Bae

   Insert

   bpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   D10A in S. pyogenes Cas9, TadA mutations describe…

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC19-MAFA-PQR-NLS-tdTomato-P2A-puro-PGK-puro

   Plasmid

   #121174

   Purpose

   Donor template for CRISPR-Cas9 mediated generation of MAFA-NLS-tdTomato reporter cell lines

   Depositor

   Timo Otonkoski

   Insert

   MAFA-PQR-NLS-tdTomato-P2A-Puro-PGK-Puro
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. CAG-ABEmax-P2A-EGFP-ires-puro

   Plasmid

   #121170

   Purpose

   CAG promoter driven expression of ABEmax base editor, EGFP and Puromycin resistance.

   Depositor

   Timo Otonkoski

   Insert

   ABEmax-P2A-EGFP-ires-puro
   

   Use

   CRISPR

   Tags

   P2A-EGFP-ires-puro

   Expression

   Mammalian

   Available Since

   July 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. plenti guide ds red

   Plasmid

   #128055

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and DsREd from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-EF1alpha-dCas9-VP64-MS2-VP64-ZsG

   Plasmid

   #192665

   Purpose

   3rd generation lenti vector encoding dCas9-VP64 with P2A MS2-VP64 and T2A ZsGreen1

   Depositor

   Jeffrey Miner

   Insert

   dCas9-VP64, MS2-VP64, ZsGreen1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9

   Promoter

   EF1a

   Available Since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCW-Tet-Cas9-miRFP670nano-PGK-Blast

    Plasmid

    #196722

    Purpose

    Tet-inducible expression of Cas9-T2A-miRFP670nano

    Depositor

    Bernhard Schmierer

    Insert

    Cas9-T2A-miRFP670nano
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG-SV40NLS and Nucleoplasmin NLS

    Promoter

    TRE, PGK

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMTF1-donor

    Plasmid

    #113829

    Purpose

    CRISPR donor plasmid to tag human transcription factor MTF1 with GFP

    Depositor

    Kevin White

    Insert

    MTF1 homology arms flanking EGFP-IRES-Neo cassette (MTF1 Human)
    

    Use

    CRISPR

    Available Since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. SpCas9_hGEM(1-110)_hSLBP(56-108)

    Plasmid

    #191388

    Purpose

    SpCas9 fused with geminin and SLBP degrons to improve HDR ratio

    Depositor

    Yannick Doyon

    Insert

    hGEM(1-110)_hSLBP(56_108)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. 1781_pAAV-U6-Ai9-Sa-gRNA1-CB-SACas9-HA-OLLAS-spA_C

    Plasmid

    #135979

    Purpose

    gRNA against the left loxP site of the Ai9 allele

    Depositor

    William Lagor

    Insert

    Ai9 gRNA1
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    HA

    Promoter

    U6

    Available Since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti-EF1alpha-dCas9-VP64-MS2-VP64-Blast

    Plasmid

    #192666

    Purpose

    3rd generation lenti vector encoding dCas9-VP64 with P2A MS2-VP64 and T2A Blast resistance marker

    Depositor

    Jeffrey Miner

    Insert

    dCas9-VP64, MS2-VP64
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9

    Promoter

    EF1a

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. p-dCas9-dDNMT3a-C706S-Hygro

    Plasmid

    #104412

    Purpose

    transient expression of dCas9-dDNMT3a fusion protein

    Depositor

    Stephan Beck

    Insert

    dDNMT3a
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    C706S

    Promoter

    CMV promoter

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDGB3 alpha1 Tnos:nptII:Pnos - SF - P35S:hCas9:Tnos (GB1656)

    Plasmid

    #160618

    Purpose

    Module for the expression of the human codon optimized Cas9 and kanamycin resistance (NptII).

    Depositor

    Diego Orzaez

    Insert

    Tnos:NptII:Pnos-SF-P35s:hCas9:tNos
    

    Use

    CRISPR

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos, 35S

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSNAI2-donor

    Plasmid

    #112378

    Purpose

    CRISPR donor plasmid to tag human transcription factor SNAI2 with GFP

    Depositor

    Kevin White

    Insert

    SNAI2 homology arms flanking EGFP-IRES-Neo cassette (SNAI2 Human)
    

    Use

    CRISPR

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. CAG-FNLS-T2A-EGFP-ires-puro

    Plasmid

    #121169

    Purpose

    CAG promoter driven expression of FNLS BE3 base editor, EGFP and Puromycin resistance.

    Depositor

    Timo Otonkoski

    Insert

    FNLS-T2A-EGFP-ires-puro
    

    Use

    CRISPR

    Tags

    T2A-EGFP-ires-puro

    Expression

    Mammalian

    Available Since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pZBTB9-donor

    Plasmid

    #112373

    Purpose

    CRISPR donor plasmid to tag human transcription factor ZBTB9 with GFP

    Depositor

    Kevin White

    Insert

    ZBTB9 homology arms flanking EGFP-IRES-Neo cassette (ZBTB9 Human)
    

    Use

    CRISPR

    Mutation

    Unknown

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZNF436-donor

    Plasmid

    #113800

    Purpose

    CRISPR donor plasmid to tag human transcription factor ZNF436 with GFP

    Depositor

    Kevin White

    Insert

    ZNF436 homology arms flanking EGFP-IRES-Neo cassette (ZNF436 Human)
    

    Use

    CRISPR

    Mutation

    rs2849028, rs646193

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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