We narrowed to 6,134 results for: nls

1. M_NLS 47kDa

   Plasmid

   #201343

   Purpose

   Nucleocytoplasmic transport probe

   Depositor

   Pere Roca-Cusachs

   Article

   Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

   Insert

   (NLS)SV40A5-EGFP-3PrA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. H_NLS 41kDa

   Plasmid

   #201340

   Purpose

   Nucleocytoplasmic transport probe

   Depositor

   Pere Roca-Cusachs

   Article

   Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

   Insert

   (NLS)SV40-EGFP-2PrA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. LLP715_L2sV3_NAND_1IF1_FRT_Rluc_FlpF1_C1-NLS_lacZ

   Plasmid

   #192415

   Purpose

   To act as a 1-input state of the split-NAND gate.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   FRT-Act2-FRT::RlucRluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. bpNLS-DddIA

   Plasmid

   #187847

   Purpose

   expresses the bpNLS-linked DddIA in mammalian cells

   Depositor

   Chengqi Yi

   Article

   Lei et al Nature. 2022 May 12. pii: 10.1038/s41586

   Insert

   bpNLS-DddIA-bpNLS-p2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pT2iC6FAST1nls2ER4(#638)

   Plasmid

   #184068

   Purpose

   cyclofen-inducible YFAST nuclear relocation via mammalian cell transfection or mRNA synthesis

   Depositor

   Sophie Vriz

   Insert

   YFAST-2nls-ERT2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   ERT2: G400V, M543A, L544A, deleted 1-281;

   Promoter

   sCMV+SP6

   Available since

   June 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHcRed-NPM1cNLSdN243-C1

   Plasmid

   #131848

   Purpose

   Expresses HcRed-NPM1c (cytoplasmic mutant, human) with the NLS domain (aa. 121-195) attached to the last 50 aminoacids in mammalian cells through transient transfection

   Depositor

   Mark Minden

   Article

   Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

   Insert

   NPM1 (NPM1 Human)
   

   Use

   Tags

   HcRed

   Expression

   Mammalian

   Mutation

   This is a deletion contruct of the cytoplasmic m…

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CTAG_NLS-SV40

   Plasmid

   #136108

   Purpose

   CTAG Nuclear localiation signal SV40 for fusion with CDS12

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   CTAG_NLS-SV40
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. PB-BE3-NLS

   Plasmid

   #119030

   Purpose

   Stably express BE3 in mammalian cells

   Depositor

   Jinsong Li

   Article

   Li et al Nat Cell Biol. 2018 Oct 1. pii: 10.1038/

   Insert

   BE3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. HA-CPI-17-wt-NLS

   Plasmid

   #100776

   Purpose

   Mammalian expression of CPI-17 nuclear

   Depositor

   Joseph Gordon

   Insert

   CPI-17-NLS (PPP1R14A Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MF85_PB-14xUAS-12x42bs-10x(ErbB2bs_ErbB2bs)-miniCMV-NLS-FKBP12F36V-ErbB2ZFR2AR39A-VP16-NLS-DHFR-IRES-mTurquoise2-PEST-BGHpA

    Plasmid

    #176629

    Purpose

    TF C self-activation cassette in MultiFate-3

    Depositor

    Michael Elowitz

    Article

    Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

    Insert

    14xUAS-12x42bs-10x(ErbB2bs_ErbB2bs)-miniCMV-NLS-FKBP12F36V-ErbB2ZFR2AR39A-VP16-NLS-DHFR-IRES-mTurquoise2-PEST-BGHpA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MF83_PB-TRE3G-6x42bs-10x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA

    Plasmid

    #176627

    Purpose

    TF B self-activation cassette in MultiFate-2.2 and MultiFate-3

    Depositor

    Michael Elowitz

    Article

    Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

    Insert

    TRE3G-6x42bs-10x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MF82_PB-TRE3G-12x42bs-10x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA

    Plasmid

    #176626

    Purpose

    TF A self-activation cassette in MultiFate-2.2 and MultiFate-3

    Depositor

    Michael Elowitz

    Article

    Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

    Insert

    TRE3G-12x42bs-10x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-S5E2-dTom-nlsdTom (AAV PHP.eB)

    Viral Prep

    #135630-PHPeB

    Purpose

    Ready-to-use AAV PHP.eB particles produced from pAAV-S5E2-dTom-nlsdTom (#135630). In addition to the viral particles, you will also receive purified pAAV-S5E2-dTom-nlsdTom plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the E2 regulatory element. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Jordane Dimidschstein

    Article

    Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

    Promoter

    Tags

    dTomato

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

14. pAAV-CAG-NLS-GFP (AAV PHP.eB)

    Viral Prep

    #104061-PHPeB

    Purpose

    Ready-to-use AAV PHP.eB particles produced from pAAV-CAG-NLS-GFP (#104061). In addition to the viral particles, you will also receive purified pAAV-CAG-NLS-GFP plasmid DNA. CAG-driven NLS-GFP expression. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Promoter

    CAG

    Tags

    GFP

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

15. pAAV-mDlx-NLS-mRuby2 (AAV PHP.eB)

    Viral Prep

    #99130-PHPeB

    Purpose

    Ready-to-use AAV PHP.eB particles produced from pAAV-mDlx-NLS-mRuby2 (#99130). In addition to the viral particles, you will also receive purified pAAV-mDlx-NLS-mRuby2 plasmid DNA. mDlx-driven NLS-mRuby2 expression. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Promoter

    mDLX5/6

    Tags

    mRuby2

    Available since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

16. pAAV-mDlx-NLS-mRuby2 (AAV Retrograde)

    Viral Prep

    #99130-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pAAV-mDlx-NLS-mRuby2 (#99130). In addition to the viral particles, you will also receive purified pAAV-mDlx-NLS-mRuby2 plasmid DNA. mDlx-driven NLS-mRuby2 expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Promoter

    mDLX5/6

    Tags

    mRuby2

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

17. pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA (AAV9)

    Viral Prep

    #192552-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA (#192552). In addition to the viral particles, you will also receive purified pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA plasmid DNA. CAG-driven expression of nuclear localized tdTomato. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Guoping Feng

    Promoter

    CAG

    Tags

    tdTomato

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

18. AAV-hSyn1-GCaMP6f-P2A-nls-dTomato (AAV1)

    Viral Prep

    #51085-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from AAV-hSyn1-GCaMP6f-P2A-nls-dTomato (#51085). In addition to the viral particles, you will also receive purified AAV-hSyn1-GCaMP6f-P2A-nls-dTomato plasmid DNA. GCaMP6f calcium sensor and bicistronic, physically separate nuclear localized dTomato expression under the Synapsin promoter. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Jonathan Ting

    Promoter

    Syn

    Tags

    nuclear dTomato (physically separate, not a fusion protein)

    Available since

    Sept. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

19. AAV-hSyn1-GCaMP6s-P2A-nls-dTomato (AAV1)

    Viral Prep

    #51084-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from AAV-hSyn1-GCaMP6s-P2A-nls-dTomato (#51084). In addition to the viral particles, you will also receive purified AAV-hSyn1-GCaMP6s-P2A-nls-dTomato plasmid DNA. GCaMP6s calcium sensor and bicistronic, physically separate nuclear localized dTomato expression under a human synapsin1 promoter. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Jonathan Ting

    Promoter

    Syn

    Tags

    nuclear dTomato (physically separate, not a fusion protein)

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

20. pLenti-UltraCas12a-6xNLS

    Plasmid

    #209023

    Purpose

    Lentiviral vector expressing human codon-optimized UltraCas12a with 6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    UltraCas12a-6xNLS-Myc-NeoR
    

    Use

    CRISPR and Lentiviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    M537R/F870L

    Promoter

    EF-1a

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge