We narrowed to 5,835 results for: pcas

1. cacnb1b pCAGGS

   Plasmid

   #206102

   Purpose

   expression of rat beta1b calcium channel auxillary subunit

   Depositor

   Annette Dolphin

   Insert

   cacnb1b (Cacnb1 Rat)
   

   Expression

   Mammalian

   Promoter

   CMV/B-actin

   Available Since

   Nov. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF1a-nSpCas9

   Plasmid

   #171691

   Purpose

   Expresses nSpCas9 in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

   Insert

   nSpCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   EF1a

   Available Since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. eSpCas9(1.1)

   Plasmid

   #138555

   Purpose

   Expresses human codon-optimized enhanced specificity SpCas9 and blasticidin resistance: EFS promoter-eSpCas9(1.1)-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   eSpCas9(1.1)
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   K848A, K1003A, R1060A

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGGs-Venus

   Plasmid

   #127346

   Purpose

   Expresses Venus constitutively via the CAGGS promoter in mammalian cells

   Depositor

   Phil Sharp

   Insert

   Venus Fluorescent Protein
   

   Expression

   Mammalian

   Promoter

   CAGGS Promoter

   Available Since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-Voltron

   Plasmid

   #119033

   Purpose

   Express Voltron in mammalian cells

   Depositor

   Eric Schreiter

   Article

   Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

   Insert

   Voltron
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS-NICD

   Plasmid

   #26891

   Depositor

   Nicholas Gaiano

   Article

   Dang et al Dev Neurosci. 2006;28(1-2):58-69.

   Insert

   NICD (Notch1 Mouse)
   

   Tags

   FLAG and b-globin polyA

   Expression

   Mammalian

   Mutation

   E2299K

   Available Since

   Nov. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pCAMKIIa-TetOn3G

   Plasmid

   #133727

   Purpose

   Plasmid contains the TetOn3G-transactivator sequence under control of a Camk2a promoter.

   Depositor

   Erin Schuman

   Article

   Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

   Insert

   TetOn3G
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   pCamk2a

   Available Since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG_mRuby2_smFP OLLAS

   Plasmid

   #59761

   Purpose

   encodes multiple copies of an epitope tag OLLAS ( not detected by anti GFP ab)

   Depositor

   Loren Looger

   Article

   Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

   Insert

   mRuby2_smFP_OLLAS
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-memRFP

   Plasmid

   #224564

   Purpose

   Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and membrane RFP reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   mRFP1
   

   Use

   Unspecified

   Tags

   Membrane localization signal

   Mutation

   Codon optimized

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-Beatrix

    Plasmid

    #167528

    Purpose

    Dual-color fluorescent reporter for the creation and detection of Cre-dependent tunable mosaics in floxed mouse lines and cells.

    Depositor

    Gian Michele Ratto

    Article

    Trovato et al Nat Commun. 2020 Dec 3;11(1):6194. doi:

    Insert

    Beatrix
    

    Use

    Cre/Lox and Mouse Targeting

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEC1.2/EC1.1_spCas9_TagRFP

    Plasmid

    #161934

    Purpose

    CRISPR plasmid with spCas9 expressed under egg cell promoter - TagRFP seed selection

    Depositor

    Christian Fankhauser

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. AB65_pCAG.Cas9-D10A.rBGpA

    Plasmid

    #199256

    Purpose

    Expression construct encoding the SpCas9-D10A nickase

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Insert

    SpCas9.D10A nickase
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CAG promoter

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-sfGFP

    Plasmid

    #125747

    Purpose

    sfGFP without iCreERT2 fusion

    Depositor

    Mario Capecchi

    Article

    Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

    Insert

    sfGFP
    

    Expression

    Mammalian

    Available Since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX459_gRNA-ROSA26_hspCas9

    Plasmid

    #193310

    Purpose

    Cas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

    Insert

    Cas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    not applicable

    Available Since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG/ATP10D-HA

    Plasmid

    #209217

    Purpose

    Mammalian expression of ATP10D

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

    Insert

    ATP10D (ATP10D Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-Positron

    Plasmid

    #129253

    Purpose

    Express Positron in mammalian cells

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

    Insert

    Positron
    

    Expression

    Mammalian

    Mutation

    D92N, E199V

    Promoter

    CAG

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. SpCas9-mSA*

    Plasmid

    #182952

    Purpose

    Express SpCas9-mSA*

    Depositor

    Wen Xue

    Article

    Liang et al Nat Commun. 2022 Jan 21;13(1):437. doi:

    Insert

    SpCas9-mSA*
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG/ATP11A-HA

    Plasmid

    #209218

    Purpose

    Mammalian expression of ATP11A

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

    Insert

    ATP11A (ATP11A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. B-Sniper SpCas9

    Plasmid

    #207361

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-Sniper SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    F539S, M763I, K890N, amino acids 1005-1013 replac…

    Promoter

    CBh

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAGGS_cAMPFIRE-L-R279E

    Plasmid

    #182286

    Purpose

    Mammalian expression of cAMPFIRE-L-R279E (non-cAMP-binding) under a CAG promotor w/ WPRE translation enhancer.

    Depositor

    Haining Zhong

    Article

    Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

    Insert

    cAMPFIRE-L-R279E (cAMP-deficient cAMPFIRE-L)
    

    Expression

    Mammalian

    Available Since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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