167,318 results

1. gTBEv4

   Plasmid

   #220620

   Purpose

   vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv4) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

   Insert

   nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
   

   Expression

   Mammalian

   Mutation

   hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…

   Promoter

   hU6, EF1a

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-Merlin S518D

   Plasmid

   #84295

   Purpose

   Expression of Merlin with a phosphomimic (aspartic acid) mutation at the Pak phosphorylation site

   Depositor

   Jonathan Chernoff

   Article

   Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

   Insert

   Merlin (NF2) (NF2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S518D

   Promoter

   CMV

   Available Since

   Oct. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENN.AAV.CAG.Flex.CaMPARI.WPRE.SV40

   Plasmid

   #100831

   Purpose

   AAV expression of CaMPARI, a photoconvertible fluorescent protein-based calcium integrator

   Depositor

   Loren Looger, Eric Schreiter

   Article

   Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

   Insert

   CaMPARI
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-PKCA D463N

   Plasmid

   #195203

   Purpose

   Mammalian expression vector for GFP tagged PKCA. Kinase-inactive mutant

   Depositor

   David Bryant, Peter Parker

   Article

   Cameron et al Nat Struct Mol Biol. 2009 Jun;16(6):624-

   Insert

   PRKCA (PRKCA Human)
   

   Expression

   Mammalian

   Mutation

   D463N

   Available Since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHiUGE HA-epitope donor ORF0

   Plasmid

   #200371

   Purpose

   Tagging endogenously expressed proteins with HA epitope, C-terminal

   Depositor

   Scott Soderling

   Article

   Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

   Insert

   HA epitope
   

   Expression

   Mammalian

   Mutation

   NA

   Available Since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBS-hsFlt3L

   Plasmid

   #25074

   Depositor

   Maria Castro

   Article

   Curtin et al J Immunol. 2006 Mar 15. 176(6):3566-77.

   Insert

   human soluble FMS-like tyrosine kinase 3 ligand (FLT3LG Human)
   

   Expression

   Bacterial

   Available Since

   July 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFBP-2_6.sensor

   Plasmid

   #162800

   Purpose

   Yeast expression of the RNA-based sensor for fructose-1,6-bisphosphate #2_6

   Depositor

   Matthias Heinemann

   Article

   Ortega et al Cell Chem Biol. 2021 Apr 22. pii: S2451-

   Insert

   2_6_RNA-device
   

   Expression

   Yeast

   Available Since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. rAAV2/MG1.2

   Plasmid

   #184541

   Purpose

   AAV packaging plasmid, expressing Rep2 and AAV-MG1.2 capsid

   Depositor

   Minmin Luo

   Article

   Lin et al Nat Methods. 2022 Jul 25. pii: 10.1038/s

   Insert

   AAV-MG1.2 capsid
   

   Use

   AAV; Helper vector for packaging

   Mutation

   changes on AAV9 capsid: K449R, Q588T; insertion o…

   Available Since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223_DKK1_WT

   Plasmid

   #82250

   Purpose

   Gateway Donor vector containing DKK1 , part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, William Hahn, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   DKK1 (DKK1 Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. YAP/TAZ-TRE-mStrawberry reporter

    Plasmid

    #158682

    Purpose

    This plasmid is a YAP/TAZ pathway reporter. It has a YAP/TAZ-responsive synthetic promoter driving the expression of mStrawberry-pGK-BSD

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    mStrawberry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Dronpa3-C1

    Plasmid

    #54655

    Purpose

    Localization: C1 Cloning Vector, Excitation: 503, Emission: 518

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Tags

    Dronpa3

    Expression

    Mammalian

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pDONR221_SLC4A7

    Plasmid

    #132204

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC4A7 (SLC4A7 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 28, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. Tol2-UAS-Dendra2

    Plasmid

    #97145

    Purpose

    For Gal4/UAS expression system

    Depositor

    Qing Deng

    Article

    Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

    Insert

    Dendra2
    

    Use

    Zebrafish

    Available Since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. EGFP-Cyclin F

    Plasmid

    #215054

    Purpose

    Expresses EGFP-Cyclin F in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CCNF (CCNF Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pATetO 6XHis T5 Exonuclease

    Plasmid

    #206209

    Purpose

    Inducible expression of T5 exonuclease

    Depositor

    Andrew Ellington

    Article

    Bhadra et al PLoS One. 2018 Aug 15;13(8):e0201681. do

    Insert

    T5 exonuclease
    

    Expression

    Bacterial

    Available Since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRGD-GmR

    Plasmid

    #74106

    Purpose

    Plasmid containing gentamicin resistance gene cassette flanked by multiple restriction sites

    Depositor

    Hans-Martin Fischer

    Article

    Ledermann et al Appl Environ Microbiol. 2016 Feb 26. pii

    Insert

    aacC1
    

    Use

    Bacterial vector based on pmb1 replicon

    Available Since

    April 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSimpleII-NLS-NmCas9-HA-NLS(s)

    Plasmid

    #47867

    Purpose

    This plasmid encode NmCas9 with two NLS and a HA tag

    Depositor

    James Thomson, Erik Sontheimer

    Article

    Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

    Insert

    NmCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLL2787

    Plasmid

    #15460

    Depositor

    Chia Lee

    Article

    Luong et al J Microbiol Methods. 2007 Jul . 70(1):18

    Insert

    phage phi11 int gene
    

    Expression

    Bacterial

    Available Since

    Oct. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. mCherry-Alpha-Actinin2-N-18

    Plasmid

    #54974

    Purpose

    Localization: Alpha Actinin, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Alpha-Actinin2 (ACTN2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pLVX-EF1alpha-2xGFP:NES-IRES-2xRFP:NLS

    Plasmid

    #71396

    Purpose

    2Gi2R assay

    Depositor

    Fred Gage

    Article

    Mertens et al Cell Stem Cell. 2015 Oct 6. pii: S1934-5

    Inserts

    2xGFP:NES
    2xRFP:NLS
    

    Use

    Lentiviral

    Tags

    IRES

    Expression

    Mammalian

    Promoter

    EF1alpha and IRES

    Available Since

    Dec. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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