167,468 results

1. pWM_12x601_15bpLinker

   Plasmid

   #157786

   Purpose

   EcoRV digestion produces 12x601 chromatin assembly construct with 15 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   12x601_15bp linker
   

   Expression

   Bacterial

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDH1-CMV-FIP200-V44D/V314D/L324D/R543D/R548D-mCherry-Hygro

   Plasmid

   #210857

   Purpose

   Lentiviral expression vector encoding FIP200 mutant-mCherry

   Depositor

   James Hurley

   Article

   Chen et al bioRxiv 2023.06.01.543278

   Insert

   FIP200 (RB1CC1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Mutation

   V44D/V314D/L324D/R543D/R548D

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLJM1-FLAG-metap2

   Plasmid

   #100510

   Purpose

   Expresses FLAG tagged metap2 in mammalian cells to make lentivirus

   Depositor

   David Sabatini

   Article

   Gu et al Science. 2017 Nov 10;358(6364):813-818.

   Insert

   METAP2 (METAP2 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Nov. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-Beta3

   Plasmid

   #140988

   Purpose

   Encodes a Gbeta subunit (GNB3) as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GBeta3 (GNB3 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti6.2_miRFP670

   Plasmid

   #113726

   Purpose

   Expresses the fluorescent protein miRFP670 in a lentiviral backbone

   Depositor

   Vanessa LaPointe

   Insert

   miRFP670
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. SPYtag-GFPnanobody

   Plasmid

   #214837

   Purpose

   This plasmid encodes a protein (SPYtag-GFPnanobody) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

   Depositor

   Hironori Funabiki

   Article

   Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.

   Insert

   SPYtag-GFPnanobody
   

   Tags

   His-TEV-SUMO-

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCherry-Parkin-IRES-zeo

   Plasmid

   #61727

   Purpose

   Expression of human Parkin fused to mCherry

   Depositor

   Douglas Green, Stephen Tait

   Article

   Tait et al Cell Rep. 2013 Nov 27;5(4):878-85. doi:

   Insert

   Parkin (PRKN Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWM_12x601_30bpLinker

   Plasmid

   #157789

   Purpose

   EcoRV digestion produces 12x601 chromatin assembly construct with 30 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   12x601_30bp linker
   

   Expression

   Bacterial

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Mutant ornithine transcarbamylase aa 30-114 deleted (OTC-delta)

   Plasmid

   #71878

   Purpose

   For mitochondrial unfolded protein response research

   Depositor

   Nicholas Hoogenraad

   Article

   Zhao et al EMBO J. 2002 Sep 2;21(17):4411-9.

   Insert

   OTC-delta (Otc Rat)
   

   Mutation

   Deleted amino acid 30-114 from full length OTC

   Available Since

   Jan. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. AICSDP-9:DSP-mEGFP

    Plasmid

    #87424

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human DSP

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

    Insert

    DSP Homology Arms with linker-mEGFP (DSP Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    March 15, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. NW11

    Plasmid

    #133443

    Purpose

    Covalently circularized nanodisc NW11 for studying membrane proteins and viral entry.

    Depositor

    Gerhard Wagner

    Article

    Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

    Insert

    NW11
    

    Expression

    Bacterial

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-SENP3

    Plasmid

    #34554

    Depositor

    Mary Dasso

    Article

    Yun et al J Cell Biol. 2008 Nov 17;183(4):589-95.

    Insert

    SENP3 (SENP3 Human)
    

    Tags

    EGFP

    Promoter

    CMV

    Available Since

    Dec. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC gRNA Shuttle

    Plasmid

    #47024

    Purpose

    Encodes a template from which gRNAs can be made via InFusion cloning. The Medicago truncatula U6.6 promoter drives the gRNA. For use in plants.

    Depositor

    Wayne Parrott

    Article

    Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

    Insert

    gRNA Shuttle
    

    Use

    CRISPR; Cas9

    Expression

    Plant

    Mutation

    G to T cloning mutation at position 323

    Promoter

    Medicago truncatula U6.6

    Available Since

    Sept. 3, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pMXs-Etv6

    Plasmid

    #131599

    Purpose

    expression of mouse Etv6 in mammalian cells

    Depositor

    Filipe Pereira, Kateri Moore

    Article

    Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

    Insert

    Etv6 (Etv6 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLVX-2xFLAG-2xSTREP-CCND3-IRES-mCherry

    Plasmid

    #172620

    Purpose

    Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D3 and mCherry in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND3 (CCND3 Human)
    

    Use

    Lentiviral

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pWL078 MCP-2xGFP

    Plasmid

    #197047

    Purpose

    pCYC-NLS-MCP-2xGFP on pRS305

    Depositor

    Robert Singer

    Article

    Li et al Nat Methods. 2022 Dec;19(12):1558-1562.

    Insert

    MCP-2xGFP
    

    Expression

    Yeast

    Promoter

    CYC1

    Available Since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. iF59 PB-TO-oNgn2-puro

    Plasmid

    #204734

    Purpose

    Improved Dox-inducible neurogenin-2; Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase.

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Optimized neurogenin-2
    

    Expression

    Mammalian

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCap-SG5-Hgy

    Plasmid

    #227580

    Purpose

    Capture plasmid with hygromycin resistance and pSG5 replicon

    Depositor

    Chengzhang Fu

    Article

    Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

    Type

    Empty backbone

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. GPR158-Tango

    Plasmid

    #66332

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR158 (GPR158 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

20. miniTurbo-His6_pET21a

    Plasmid

    #107178

    Purpose

    expresses His6-tagged miniTurbo in bacteria

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurbo (BirA mutant)
    

    Expression

    Bacterial

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. mU6-sgRNA-CLTA-mCherry

    Plasmid

    #240637

    Purpose

    Vector for expressing an sgRNA targeting CLTA promoter from the mouse U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter.

    Depositor

    James Nuñez

    Article

    Xu et al Nat Commun. 2025 Aug 26;16(1):7948. doi:

    Insert

    puro-T2A-mCherry
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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22. pcDNA3 HA E2F3A

    Plasmid

    #236437

    Purpose

    transient overexpression of E2F3A in mammalian cells

    Depositor

    Michele Pagano

    Article

    Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

    Insert

    E2F3A (E2F3 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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23. pFB1_hMSH6

    Plasmid

    #129424

    Purpose

    Expression human MSH6 protein using baculovirus

    Depositor

    Peggy Hsieh

    Article

    Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

    Insert

    human MSH6 (MSH6 Human)
    

    Expression

    Insect

    Promoter

    Polyhedrin promoter

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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24. LAMP1-APEX2

    Plasmid

    #215555

    Purpose

    Stable, inducible expression in lysosome

    Depositor

    Nevan Krogan

    Article

    Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

    Insert

    LAMP1-EGFP-Flag tag-APEX2-NES
    

    Use

    Lentiviral

    Available Since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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25. pAAV-syn-dLight1.3b

    Plasmid

    #135762

    Purpose

    For expression of the genetically encoded dopamine sensor dLight1.3b

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Has Service

    AAV9

    Insert

    dLight1.3b
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mammalian

    Promoter

    Synapsin

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. LentiCRISPR-V2_hMSH2

    Plasmid

    #186155

    Purpose

    sgRNA targeting human MSH2 gene

    Depositor

    David McFadden

    Article

    Povedano et al Cell Chem Biol. 2019 Sep 19;26(9):1315-1

    Insert

    MSH2 (MSH2 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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27. pLENTI6.3/AR-GC-E2325

    Plasmid

    #85128

    Purpose

    exogenous AR expression

    Depositor

    Karl-Henning Kalland

    Article

    Olsen et al BMC Cancer. 2016 Jul 4;16:377. doi: 10.1

    Insert

    androgen receptor (AR Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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28. pAAV-hSyn-hM4D(Gi)-mCherry (AAV Retrograde)

    Viral Prep

    #50475-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-hM4D(Gi)-mCherry (#50475). In addition to the viral particles, you will also receive purified pAAV-hSyn-hM4D(Gi)-mCherry plasmid DNA. CNO-induced neuronal silencing. Synapsin-driven with mCherry reporter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Bryan Roth

    Promoter

    Syn

    Tags

    mCherry

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

29. pTet-IRES-EGFP

    Plasmid

    #64238

    Purpose

    Lentiviral, doxycycline-inducible system to express transgene of interest and EGFP.

    Depositor

    Maria Lung

    Article

    Shuen et al Cancer Gene Ther. 2015 Feb 27. doi: 10.1

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Promoter

    pTet

    Available Since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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30. pcDNA3-Cyto-GCaMP3

    Plasmid

    #64853

    Purpose

    Cytosol-targeted version of the Ca2+ indicator GCaMP3.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    GCaMP3
    

    Tags

    Nuclear export signal (NES)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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