We narrowed to 18,995 results for: Met;

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pJT11

Plasmid

#98308

Purpose

Overexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Citrus limon limonene synthase LIS1 under the control of GAL1/GAL2 promoter

Depositor

Claudia Vickers

Article

Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(

Insert

TRPL3ClLIS1>TRPL41B

Expression

Yeast

Available Since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pXWJ109HgAmp30E

Plasmid

#123149

Purpose

Mercury sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t

Depositor

Baojun Wang

Article

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

J109, PmerT, PhrpLE

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pJCC_075 AsCas12a D908A

Plasmid

#171668

Purpose

For bacterial expression of AsCas12a D908A (RuvC-inactivating mutation) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

AsCas12a D908A

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

changed aspartate 908 to alanine

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pBEAST-pBen-sfGFP

Plasmid

#114598

Purpose

"Output expression of sfGFP under the activation of BenR transcription factor for cell-free expression"

Depositor

Jerome Bonnet

Article

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

sfGFP

Use

Synthetic Biology; Cell-free protein synthesis

Promoter

PBen

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pT3TS-HiFi-nCas9n-A72

Plasmid

#125646

Purpose

micro-injection of poly-adenylated HiFi-nCas9n RNA

Depositor

Jason Berman

Article

Prykhozhij et al Methods Mol Biol. 2020;2115:385-405. doi

Insert

HiFi-nCas9n

Use

Rna in vitro transcription vector

Mutation

R691A from wild-type Cas9

Promoter

T3

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pZ_lacO-cP-AOX1-NpgA

Plasmid

#126731

Purpose

yeast plasmid overexpressing NpgA

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

npgA

Tags

6xHis

Expression

Yeast

Mutation

G951A,C957A

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBEAST-BenR

Plasmid

#114597

Purpose

"Strong constitutive expression of transcription factor, BenR, for cell-free expression"

Depositor

Jerome Bonnet

Article

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

BenR

Use

Synthetic Biology; Cell-free protein synthesis

Promoter

OR2-OR1-Pr

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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FH-TET3-CXXCmut-pEF

Plasmid

#127895

Purpose

Expresses ten-eleven-translocation 3 (TET3) containing CXXC mutation 80THQ82 to AAA

Depositor

Patrick Hogan, Anjana Rao

Article

Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

Insert

TET3-CxxCmut (TET3 Human)

Tags

HA and Flag

Expression

Mammalian

Promoter

EF-1a

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pALS343 (pGEX-2TP-cTevHis-Gibson/MCS)

Plasmid

#139788

Purpose

Vector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHis

Depositor

Arneet Saltzman

Article

Saltzman et al Genetics. 2018 Nov;210(3):907-923. doi:

Type

Empty backbone

Tags

6xHis (cleavable by TEV protease) and GST (cleava…

Expression

Bacterial

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pT2ADW-lox-mCherry-hyBRET-ERK-NLS

Plasmid

#170290

Purpose

For generating the conditional transgenic mice expressing BRET-FRET hybrid biosensor of ERK.

Depositor

Michiyuki Matsuda

Article

Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

Insert

lox-mCherry-lox-hyBRET-ERK-NLS

Expression

Mammalian

Available Since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 NSP2

Plasmid

#158353

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP2 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-SARS-CoV-2 Orf10

Plasmid

#158380

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 Orf10 (ORF10 )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti-X1-Neo-NDUFB2

Plasmid

#139841

Purpose

Lentiviral expression of human NDUFB2

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

NDUFB2 (NDUFB2 Human)

Use

Lentiviral

Available Since

July 6, 2020

Availability

Academic Institutions and Nonprofits only

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pRW006-SM-destination-pro35S-Cas9

Plasmid

#104439

Purpose

Destination vector expressing plant-codon-optimized Cas9 under 35S promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free

Depositor

Detlef Weigel

Article

Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

Type

Empty backbone

Use

CRISPR

Expression

Bacterial and Plant

Promoter

35S

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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35S::Cel1SP-GeNL/pCAMBIA1301

Plasmid

#190069

Purpose

Expressing bioluminescent pH indicator-GeNL- in Arabidopsis thaliana apoplast

Depositor

Takeharu Nagai

Article

Tran et al Plant Cell Environ. 2022 Jul 21. doi: 10

Insert

Cel1 signal peptide-Green-enhanced Nano-lantern

Expression

Plant

Promoter

CaMV 35S

Available Since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pHIE050 ZF43x0 mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

Plasmid

#138731

Purpose

Integration Vector, assembled with pHIE042 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x0

Use

Synthetic Biology

Expression

Mammalian

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pegRNA_HEK4_20-7

Plasmid

#201974

Purpose

Express a pegRNA used for 20bp tag insertion at HEK4 site with a 7bp homology arm

Depositor

Scot Wolfe

Article

Liang et al Nat Methods. 2023 Jun;20(6):898-907. doi

Insert

pegRNA_HEK4_20-7

Expression

Mammalian

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 M

Plasmid

#158369

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 M (M )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pAc5.1B-lambdaN-HA-DmPAN3-W707AG711E-dsRNAres_Y

Plasmid

#148087

Purpose

Insect Expression of DmPAN3-W707AG711E-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN3-W707AG711E-dsRNAres (CG11486 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 NSP12

Plasmid

#158363

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP12 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-SARS-CoV-2 NSP14

Plasmid

#158365

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP14 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pHIE053 ZF43x6-C mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

Plasmid

#138734

Purpose

Integration Vector, assembled with pHIE045 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x6-Compact

Use

Synthetic Biology

Expression

Mammalian

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 NSP11

Plasmid

#158362

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP11 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pRK5-HA-SAMTOR (G172A)

Plasmid

#100514

Purpose

Expresses HA tagged SAMTOR G172A mutant in mammalian cells

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

SAMTOR (BMT2 Human)

Tags

HA

Expression

Mammalian

Mutation

G172A

Available Since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pROF394

Plasmid

#155333

Purpose

Expression of PULSE-controlled LFY-VP16-2A-RLuc

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

(etr)8-(C120)5-PhCMVmin-LFY-VP16-NLS-2A-RLuc-TSV40

Use

Synthetic Biology; Eukaryotic expression

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pET-17b_Unc104-KIF5B-HALO

Plasmid

#169996

Purpose

Ultra processive kinesin KIF1A fused with KIF5B stalk for dimerization and HALO-tag for labeling

Depositor

Ron Vale

Article

Niekamp et al Nat Methods. 2020 Apr;17(4):437-441. doi

Insert

Kinesin-like protein Unc-104 fused with KIF5B

Tags

HALO-tag, 6xHIS

Expression

Bacterial

Promoter

T7

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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tdEos-VSVG-16

Plasmid

#57693

Purpose

Localization: Golgi/ER Transport, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

VSVG

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pTS2410-Tier1-PhCMV-Myr_mTagBFP2

Plasmid

#169535

Purpose

Tier-1 vector encoding PhCMV-controlled plasma membrane-tethered mTagBFP2 (PhCMV-myr-mTagBFP2-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven membrane-localized monomeric Tag blue fluorescent protein 2

Expression

Mammalian

Promoter

PhCMV

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pENV8(GAAA)-GFPuv

Plasmid

#70128

Purpose

Negative control cobalamin riboswitch reporter vector for use in E. coli; deletion of regulatory kissing loop interaction by conversion of L5 to GAAA tetra loop

Depositor

Robert Batey

Article

Johnson JE et al Nature. 2012 Dec 6;492(7427):133-7. doi:

Insert

kissing loop regulatory mutant of environmental cobalamin riboswitch sequence (env8)

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Promoter

tac

Available Since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
MDH1-PGK-GFP-miR9

Plasmid

#25036

Depositor

Bob Weinberg

Article

Ma et al Nat Cell Biol. 2010 Mar . 12(3):247-56.

Insert

mir-9-3 (MIR9-3 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
pENN.AAV.CB7.CI.PM20D1-flag.WPRE.rBG

Plasmid

#132682

Purpose

AAV plasmid encoding mouse PM20D1 with C-terminal flag tag

Depositor

Jonathan Long

Article

Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

Insert

Peptidase M20 domain containing 1 (Pm20d1 Mouse)

Use

AAV

Tags

6xHIS and Flag

Promoter

Chicken b-actin

Available Since

Nov. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pPMVAugw

Plasmid

#98300

Purpose

Enhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of CUP1 promoter.

Depositor

Claudia Vickers

Article

Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

Insert

TEFM1EfmvaE>TEBS1

Expression

Yeast

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
ptdEos-CLC

Plasmid

#38009

Depositor

Xiaowei Zhuang

Article

Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

Inserts

Clathrin, light polypeptide (LCa) (Clta Mouse)
Eos FP, tandem dimer

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Psicheck2 Sox4 full length 3'UTR

Plasmid

#26989

Depositor

Joan Massague

Article

Tavazoie et al Nature. 2008 Jan 10. 451(7175):147-52.

Insert

Sox4 Full length 3' UTR (SOX4 Human)

Use

Luciferase

Tags

Renilla Luciferase

Expression

Mammalian

Available Since

Jan. 10, 2011

Availability

Academic Institutions and Nonprofits only
Myc-Foxo1-WT 6KQ

Plasmid

#17559

Depositor

Domenico Accili

Article

Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

Insert

Foxo1 6KQ (Foxo1 Mouse)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2008

Availability

Academic Institutions and Nonprofits only
MAC-SARS-CoV-2 Orf3a

Plasmid

#158372

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 Orf3a (ORF3a )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCytERM-hfYFP

Plasmid

#186520

Purpose

Visualization of the ER in cells using hyperfolder YFP (compatible with OSER assay)

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Hyperfolder YFP

Tags

Cytochrome p450 first 29 animo acids (Oryctolagus…

Expression

Mammalian

Mutation

mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs_FLAG-SFXN3

Plasmid

#110637

Purpose

Retroviral expression of Flag-SFXN3 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Science. 2018 Nov 16;362(6416). pii: 362

Insert

SFXN3 (SFXN3 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-myc-ΔD-box-CycA-GFP (525)

Plasmid

#39856

Depositor

Jonathon Pines

Article

den Elzen et al J Cell Biol. 2001 Apr 2;153(1):121-36.

Insert

Cyclin A2 (CCNA2 Human)

Tags

GFP and Myc

Expression

Mammalian

Mutation

L2V; Δ destruction box = deletion of aa47-55

Available Since

Jan. 28, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pGREAT18

Plasmid

#170906

Purpose

GoldenBraid Double Transcriptional Unit - pAtUBQ10_E-Luc_AtHSP18.2t+pNOS_Red-F_NOSt

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

E-Luc

Use

Luciferase and Synthetic Biology

Expression

Plant

Mutation

c.375C>T silent mutation to remove BbsI site (…

Promoter

pAtUBQ10

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

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