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Showing: 7951 - 7980 of 160936 results

  2. pLTR.gp140/EGFP.Revdelta38/DsRed

    Plasmid
    #115775
    Purpose
    HIV splicing reporter allows the assessment of the effect of LRAs on HIV-1 transcription and splicing.
    Depositor
    Damian Purcell
    Article
    Khoury et al J Vis Exp. 2019 Jan 22;(143). doi: 10.37
    Insert
    Envelope fused to EGFP (gp140-EGFP) and non-functional Rev protein fused to DsRed fluorescent protein (Rev-delta38-DsRed).
    Use
    Tags
    EGFP; dsReD
    Expression
    Mammalian
    Mutation
    gp140 uncleaved (1190-91aa, KR > TG; truncated…
    Promoter
    HIV-1 (LTR)
    Available since
    Oct. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    115775-plasmid-map-sequence-id-224438

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  6. pAAV-Ef1a-DIO eNpHR 3.0-EYFP (AAV1)

    Viral Prep
    #26966-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-Ef1a-DIO eNpHR 3.0-EYFP (#26966). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO eNpHR 3.0-EYFP plasmid DNA. EF1a-driven, cre-dependent eNpHR 3.0-EYFP for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Gradinaru et al Cell. 2010 Apr 2. 141(1):154-65.
    Promoter
    EF1a
    Tags
    EYFP (Cre-dependent)
    Available since
    Feb. 14, 2018
    Availability
    Academic Institutions and Nonprofits only

  7. pET22B_SA_PcrA

    Plasmid
    #102999
    Purpose
    The plasmid is used to express Staphylococcus aureus PcrA, a DNA helicase, with N-terminal His-tag and Gly-Gly linker
    Depositor
    Martin Webb
    Insert
    SA_PcrA
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    PcrA 199-2423
    Promoter
    T7
    Available since
    May 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    102999-plasmid-map-sequence-id-199646

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  8. pAX2

    Plasmid
    #117398
    Purpose
    allelic exchange vector with GFP merodiploid tracker, temperature-sensitive origin of replication, and TetR-controlled kill switch
    Depositor
    Karen Guillemin
    Article
    Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    117398-plasmid-map-sequence-id-230535

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  13. PGKp-GFP-YAP (5SA)

    Plasmid
    #174174
    Purpose
    Lentiviral vector to constitutively express overactive YAP (5 LATS phosporylation sites mutated to Ala) under control of the human PGK promoter.
    Depositor
    Rod Bremner
    Article
    Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610
    Insert
    YAP (YAP1 Human)
    Use
    Lentiviral
    Tags
    3x FLAG tag
    Expression
    Mammalian
    Mutation
    Five LATS phosphorylation sites (S61, S109, S127,…
    Promoter
    PGK
    Available since
    Aug. 19, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    174174-plasmid-map-sequence-id-340277

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  16. pAAV_hSyn1-SIO-stGtACR2-FusionRed (AAV Retrograde)

    Viral Prep
    #105677-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV_hSyn1-SIO-stGtACR2-FusionRed (#105677). In addition to the viral particles, you will also receive purified pAAV_hSyn1-SIO-stGtACR2-FusionRed plasmid DNA. Synapsin-driven, Cre-dependent, soma-targeted anion-conducting channelrhodopsin fused to FusionRed for optogenetic inhibition. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ofer Yizhar
    Article
    Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1
    Promoter
    Syn
    Tags
    FusionRed (Cre-dependent)
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only

  19. pDule2 - Mj Acd A9

    Plasmid
    #197652
    Purpose
    E. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pair
    Depositor
    Ryan Mehl
    Article
    Sungwienwong et al Org Biomol Chem. 2017 May 3;15(17):3603-
    Inserts
    Mj Acd A9 synthetase
    Methanocaldococcus jannaschii tRNA
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32A, L65D, F108L, Q109E, D158S, L162T
    Promoter
    GlnS and lpp
    Available since
    March 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    197652-plasmid-map-sequence-id-388215

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  24. rAAV2/MG1.1

    Plasmid
    #184540
    Purpose
    AAV packaging plasmid, expressing Rep2 and AAV-MG1.1 capsid
    Depositor
    Minmin Luo
    Article
    Lin et al Nat Methods. 2022 Jul 25. pii: 10.1038/s
    Insert
    AAV-MG1.1 capsid
    Use
    AAV; Helper vector for packaging
    Tags
    Expression
    Mutation
    changes on AAV9 capsid: K449R, A587L, Q588M; inse…
    Promoter
    Available since
    July 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    184540-plasmid-map-sequence-id-386238

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  25. pG1C

    Plasmid
    #71740
    Purpose
    Modular shuttle vector for Geobacillus and E. coli
    Depositor
    Tom Ellis
    Article
    Reeve et al ACS Synth Biol. 2016 Jul 1.
    Type
    Empty backbone
    Use
    Synthetic Biology; Shuttle vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71740-plasmid-map-sequence-id-130141

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  28. pAAV-Ef1a-Con/Fon-oScarlet (AAV8)

    Viral Prep
    #137136-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Con/Fon-oScarlet (#137136). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Fon-oScarlet plasmid DNA. EF1a-driven, Cre and Flp-dependent expression of oScarlet. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    EF1a
    Tags
    oScarlet (Cre and Flp-dependent)
    Available since
    April 5, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 7951 - 7980 of 160936 results