167,318 results

1. MS2-p65-HSF1-P2A-mCherry

   Plasmid

   #184440

   Purpose

   MS2 coat protein fused with p65 and hsf1

   Depositor

   Rhys Allan

   Article

   Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

   Insert

   MS2-p65-hsf1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF2193

   Plasmid

   #141964

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   TFAM (TFAM Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. BFP-Rab5CA (Q79L)

   Plasmid

   #205705

   Purpose

   Mammalian expression of BFP-tagged mutant Rab5CA

   Depositor

   Randy Schekman

   Article

   Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

   Insert

   RAB5A (RAB5A Human)
   

   Expression

   Mammalian

   Mutation

   Q79L

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET MBP GFP LIC cloning vector (MBP-msfGFP)

   Plasmid

   #29750

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   6xHis, AviTag (biotin), MBP, SiriusGFP, and TEV c…

   Expression

   Bacterial

   Available Since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCri System

   Plasmid Kit

   #1000000058

   Purpose

   pCri System: plasmids for cytoplasmic and periplasmic/extracellular heterologous protein expression in Escherichia coli, Bacillus subtilis, and Pichia pastoris.

   Depositor

   F Xavier Gomis-Ruth

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pESC-MET

   Plasmid

   #222482

   Purpose

   Co-expression of two genes in yeast with FLAG and MYC tags and MET15 selection

   Depositor

   Tetsuo Matsuzaki, Kiyofumi Yamada

   Article

   Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

   Type

   Empty backbone

   Tags

   FLAG and Myc

   Expression

   Yeast

   Available Since

   Oct. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pESC-LYS

   Plasmid

   #222483

   Purpose

   Co-expression of two genes in yeast with FLAG and MYC tags and LYS2 selection

   Depositor

   Tetsuo Matsuzaki, Kiyofumi Yamada

   Article

   Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

   Type

   Empty backbone

   Tags

   FLAG and Myc

   Expression

   Yeast

   Available Since

   Oct. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIVT-R2Tg-WT

   Plasmid

   #238105

   Purpose

   For IVT of wildtype R2Tg editor, codon optimized for mammalian expression.

   Depositor

   Feng Zhang

   Article

   Edmonds et al Nat Commun. 2025 Jul 2;16(1):6079. doi:

   Insert

   Wildtype R2Tg, mammalian codon optimized
   

   Use

   For ivt

   Available Since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmEGFP_a_tubulin_C1

   Plasmid

   #21039

   Depositor

   Daniel Gerlich

   Article

   Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

   Insert

   alpha Tubulin (TUBA1B Human)
   

   Tags

   pmEGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 11, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAd/CMV/DAX1-IRES-nEGFP

    Plasmid

    #29752

    Depositor

    Edward McCabe

    Insert

    dosage-sensitive sex reversal, adrenal hypoplasia critical region, on chromosome X, gene 1 (NR0B1 Human)
    

    Tags

    EGFP, IRES, and Nuclear localization sequence

    Expression

    Mammalian

    Mutation

    No mutations; Reporter is nuclear localized EGFP;…

    Available Since

    Oct. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSFS2A-CaHygB

    Plasmid

    #189564

    Purpose

    CaHygB hygromycin resistance gene in the pSFS2A flipper backbone for genetic manipulation of Candida albicans

    Depositor

    Richard Bennett

    Article

    Park et al mSphere. 2022 Aug 15:e0034722. doi: 10.1

    Insert

    HygB
    

    Expression

    Yeast

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti6MFV SLC25A4

    Plasmid

    #194133

    Purpose

    Expresses ANT1 (SLC25A4) protein in mammalian cells

    Depositor

    Jun Liu

    Insert

    SLC25A4 (SLC25A4 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFlag-CMV2-Brd4 del ET

    Plasmid

    #21938

    Depositor

    Eric Verdin

    Article

    Bisgrove et al Proc Natl Acad Sci U S A. 2007 Aug 21. 1

    Insert

    BRD4 (BRD4 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    aa 1-1362 only, ET domain deleted

    Available Since

    Sept. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

14. AAVS1_gRNA

    Plasmid

    #196139

    Purpose

    gRNA targeting the AAVS1 locus in a third generation Cas9 backbone with GFP

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    AAVS1 gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV CEPIA3mt

    Plasmid

    #58219

    Purpose

    Green fluorescent indicator for calcium imaging in the mitochondria (moderately low affinity variant)

    Depositor

    Masamitsu Iino

    Article

    Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

    Insert

    CEPIA3mt
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    GCaMP2 N263Y E282V E334D M339L

    Promoter

    CMV

    Available Since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC19 Giles integrase

    Plasmid

    #170049

    Purpose

    pUC19 with Giles integrase

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Insert

    GIles Integrase
    

    Expression

    Bacterial

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. T7-phiC31-nos3'UTR

    Plasmid

    #182192

    Purpose

    in vitro transcription of phiC31 integrase mRNA

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

    Insert

    phiC31 integrase
    

    Use

    In vitro transcription

    Available Since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CAG-dLight1.1 (AAV5)

    Viral Prep

    #111067-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-CAG-dLight1.1 (#111067). In addition to the viral particles, you will also receive purified pAAV-CAG-dLight1.1 plasmid DNA. CAG-driven expression of dLight1.1 dopamine sensor. This sensor is recommended for photometry in vivo. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Available Since

    Dec. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

19. Lenti-multi-CRISPR

    Plasmid

    #85402

    Purpose

    Lentiviral vector for the delivery of multiple sgRNAs targeting different genes with constitutive Cas9 expression

    Depositor

    Qin Yan

    Article

    Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Jan. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. qTAG-N-Puro-mStayGold-ACTB

    Plasmid

    #227320

    Purpose

    Donor template for Puro-2A-mStayGold insertion into the N-terminus of the ACTB locus. For actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB (Addgene #207748)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    ACTB Homology Arms flanking a Puro-2A-mStayGold Cassette (ACTB Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pLX304-IRES-Td

    Plasmid

    #176296

    Purpose

    pLX304 backbone with an IRES-Tdtomato cassette subcloned into the NheI-AgeI sites

    Depositor

    William Kaelin

    Article

    Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCLIP-tag (m) Vector

    Plasmid

    #101136

    Purpose

    Expression of protein of interest with CLIP-Tag in Mammalian cells

    Depositor

    New England Biolabs, Ana Egana

    Type

    Empty backbone

    Tags

    CLIP-tag (CLIP10)

    Expression

    Mammalian

    Available Since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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23. pLX304_CMV::mCherry-LgBiT-FLAG-NES

    Plasmid

    #199715

    Purpose

    Expresses LgBiT, fused to mCherry, in the cytosol of mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.

    Depositor

    Alice Ting

    Article

    Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

    Insert

    mCherry-LgBiT-FLAG-NES
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. pLV CMV FST-FLAG

    Plasmid

    #175167

    Purpose

    Lentiviral expression of human FST-FLAG

    Depositor

    Larry Gerace

    Insert

    FST (FST Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. pCDNA3-HA-human MYCN

    Plasmid

    #74163

    Purpose

    Mammalian expression of human MycN

    Depositor

    Martine Roussel

    Article

    Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

    Insert

    MycN (MYCN Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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26. EGFP-HsBIN1_tv8

    Plasmid

    #176012

    Purpose

    Mammalian expression of Human EGFP-Bin1 (transcriptional variant 8, muscle specific). Parton lab clone HBH

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    Bin1
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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27. pDONR223_FYN_WT

    Plasmid

    #82211

    Purpose

    Gateway Donor vector containing FYN , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Jesse Boehm, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    FYN (FYN Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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28. Pepper-fused sgRNA

    Plasmid

    #217520

    Purpose

    Expresses Pepper-fused sgRNA in mammalian cells with U6 promoter

    Depositor

    Xing Li

    Article

    Zhang et al Nat Commun. 2024 Jan 31;15(1):934. doi:

    Insert

    Pepper-fused sgRNA for spCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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29. pENTR-AIF

    Plasmid

    #16182

    Depositor

    Huda Zoghbi

    Article

    Lim et al Cell. 2006 May 19. 125(4):801-14.

    Insert

    Apoptosis-inducing factor (AIFM1 Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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30. pCDF-GFPplus

    Plasmid

    #172718

    Purpose

    Expression of a fast folding GFP variant GFP plus

    Depositor

    Tilmann Weber

    Article

    Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

    Insert

    GFP plus
    

    Expression

    Bacterial

    Available Since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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