Skip to main content
Addgene
Showing: 521 - 540 of 23589 results

  10. pAAV-Ef1a-FAS-NES-jRGECO1a-WPRE

    Plasmid
    #141236
    Purpose
    AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of jRGECO1a (jRGECO1a will not be expressed in mammalian cells that express Cre recombinase)
    Depositor
    Guohong Cui
    Article
    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do
    Insert
    NES-jRGECO1a
    Use
    AAV and Cre/Lox; Cre-off
    Tags
    6xHIS tag and nuclear export signal
    Expression
    Mutation
    Promoter
    human elongation factor-1 alpha (EF-1 alpha)
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    141236-plasmid-map-sequence-id-432423

    Enlarge

  14. 3xNLS-SGFP2(T65G)-T2A-SGFP2-3xNES

    Plasmid
    #60492
    Purpose
    Co-expresses two green fluorescent protein variants, a short fluorescence lifetime GFP variant in the nucleus and a GFP variant with a long lifetime in the cytoplasm
    Depositor
    Dorus Gadella
    Article
    Joosen et al J Microsc. 2014 Sep 1. doi: 10.1111/jmi.
    Inserts
    3xnls-SGFP2(T65G)
    SGFP2-3xNES
    Use
    Tags
    SGFP2 and SGFP2(T65G)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    60492-plasmid-map-sequence-id-96974

    Enlarge

  15. AAV-CAG-FLEX-NES-jGCaMP8f-WPRE

    Plasmid
    #186044
    Purpose
    AAV-mediated cytosolic expression of jGCaMP8f under the CAG promoter, Cre-dependent expression
    Depositor
    Loren Looger
    Insert
    NES-jGCaMP8f
    Use
    AAV
    Tags
    NES-6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    186044-plasmid-map-sequence-id-370163

    Enlarge

  19. p663-UBC-2xV5-miniTurbo-NES-STOP_IDG-K

    Plasmid
    #158161
    Purpose
    Gateway destination clone of miniTurbo-NES (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    2xV5-miniTurbo-NES
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    158161-plasmid-map-sequence-id-309972

    Enlarge

Showing: 521 - 540 of 23589 results