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IQGAP1_pLX307

Plasmid

#98346

Purpose

Lentiviral expression of IQGAP1

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

IQGAP1 (IQGAP1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-SynI-CreOn-FlpOff-Kir2.1MUT-P2A-EGFP

Plasmid

#176279

Purpose

Viral vector for co-expression of non-functional Kir2.1 and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

Depositor

Martin Riccomagno

Article

Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

Kir2.1MUT-P2A-EGFP (Kcnj2 Mouse, Synthetic)

Use

AAV and Cre/Lox

Tags

Myc

Expression

Mammalian

Mutation

GYG to AAA (aa144-146)

Promoter

human Synapsin I

Available Since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-EGF-ScNeo

Plasmid

#209893

Purpose

To monitor the status of EGF, the plasmid encodes a recombinant EGF fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

EGF-ScNeo (EGF Human)

Use

Lentiviral

Tags

GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-HBEGF-ScNeo

Plasmid

#209894

Purpose

To monitor the status of HB-EGF, the plasmid encodes a recombinant HB-EGF fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

HBEGF-ScNeo (HBEGF Human)

Use

Lentiviral

Tags

mNeonGreen and mScarlet

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSOX9-dTAG-mNeonGreen-V5

Plasmid

#194971

Purpose

AAV vector for knocking in a C-terminal tag (dTAG-mNeonGreen-V5) for human SOX9

Depositor

Joanna Wysocka

Article

Naqvi et al Nat Genet. 2023 May;55(5):841-851. doi:

Insert

SRY-box transcription factor 9 (SOX9 Human)

Use

AAV

Tags

dTAG-mNeonGreen-V5

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO5d.EFS.SpCas9.P2A.BSD

Plasmid

#57821

Purpose

Lentiviral Vector for SpCas9 Expression without sgRNA, Blasticidin resistance, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
EFS (EEF1A1 Human)
P2A-BSD

Use

CRISPR and Lentiviral

Tags

FLAG

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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AID-BE3

Plasmid

#100803

Purpose

Expresses AID-BE3 in mammalian cells

Depositor

David Liu

Article

Komor et al Sci Adv. 2017 Aug 30;3(8):eaao4774. doi:

Insert

activation induced cytidine deaminase (AICDA S. pyogenes, Human)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223-MEKDD

Plasmid

#31202

Purpose

Gateway entry vector containing MEKDD.

Depositor

David Root

Article

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Insert

MAPKK1 (MAP2K1 Human)

Use

Gateway donor vector

Mutation

S218D S222D

Available Since

June 24, 2011

Availability

Academic Institutions and Nonprofits only

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pGS_128

Plasmid

#160651

Purpose

Expresses Human APOE3 with a yeast secretory signal peptide under the control of a beta-estradiol inducible promoter

Depositor

Susan Lindquist, Li-Huei Tsai

Article

Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

Insert

Apolipoprotein E e3 (APOE Human)

Tags

Kar2 signal sequence

Expression

Yeast

Mutation

e3 allele

Promoter

pZ estradiol inducible promoter

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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TDG004_pLenti_CMVtight_Kir2.1_CFP

Plasmid

#178820

Purpose

tet-on expression of an inward-rectifier potassium channel Kir2.1 carrying an CFP tag

Depositor

Adam Cohen

Article

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

Kir2.1_CFP (Kcnj2 Mouse)

Use

Lentiviral

Tags

CFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV-BCL2

Plasmid

#163611

Purpose

Overexpression of human BCL2

Depositor

Daniel Hodson

Article

Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

Insert

BCL2 (BCL2 Human)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST-ERBB2-v2

Plasmid

#154895

Purpose

Expresses ERBB2 (a.k.a HER2) fused to Venus fragment 2 (v2) for use in bimolecular fluorescence complementation (BiFC) assays

Depositor

Johanna Ivaska

Article

Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

Insert

ERBB2 (ERBB2 Human)

Tags

Venus fragment 2 (v2)

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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pSecTaq2-hProS-Myc-6xHis

Plasmid

#226524

Purpose

Expresses TAM Receptor Ligand human PROS1

Depositor

Raymond Birge, Sergei Kotenko

Article

Geng et al Front Immunol. 2017 Nov 10;8:1521. doi:

Insert

Human PROS1 (PROS1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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CSNK1A1_pLX307

Plasmid

#98326

Purpose

Lentiviral expression of CSNK1A1

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

CSNK1A1 (CSNK1A1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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TOPO-HIF1A

Plasmid

#226452

Purpose

For subcloning of human HIF1A promoter or for assays using M13 phage

Depositor

Glen Borchert

Article

DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Insert

HIF1A (HIF1A Human)

Use

Cloning vector

Mutation

No

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pGS_107

Plasmid

#160650

Purpose

Expresses Human APOE4 with a yeast secretory signal peptide under the control of the yeast Gal promoter

Depositor

Susan Lindquist, Li-Huei Tsai

Article

Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

Insert

Apolipoprotein E e4 (APOE Human)

Tags

Kar2 signal sequence

Expression

Yeast

Mutation

e4 allele

Promoter

GAL1

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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eGFP-proα2(I)-G610C

Plasmid

#119827

Purpose

Expresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and GFP between signal sequence and exon 6

Depositor

Sergey Leikin

Article

Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

Insert

Type I procollagen α2 chain (Col1a2 Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

Col1a2 exons 2-5 replaced by fluorescent tag, Gly…

Promoter

CMV

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pCR-XL-rAkap9

Plasmid

#196867

Purpose

Cloned rat (r) A-Kinase Anchoring Protein 9 (Akap9) ORF. Used as a template for Akap9 containing constructs

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Akap9 (Akap9 Rat)

Expression

Bacterial

Promoter

Lac promoter and T7 promoter

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGS_129

Plasmid

#160652

Purpose

Expresses Human APOE4 with a yeast secretory signal peptide under the control of a beta-estradiol inducible promoter

Depositor

Susan Lindquist, Li-Huei Tsai

Article

Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

Insert

Apolipoprotein E e4 (APOE Human)

Tags

Kar2 signal sequence

Expression

Yeast

Mutation

e4 allele

Promoter

pZ estradiol inducible promoter

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA6-N-3XFLAG-Ubxd8

Plasmid

#123599

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

Fas associated factor family member 2 (Faf2 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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