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Showing: 8191 - 8220 of 160955 results

  1. pBAD-6xHis-AviTag-ALFAtag-eGFP

    Plasmid
    #195854
    Purpose
    Bacterial expression vector encoding 6xHis-AviTag-ALFAtag-eGFP. Co-expression in cells with biotin ligase (BirA) results in efficient biotinylation of the AviTag sequence.
    Depositor
    John Ngo
    Article
    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103
    Insert
    6xHis-AviTag-ALFAtag-eGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    195854-plasmid-map-sequence-id-383965

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  7. pCS2 GFP-GSK3mut-MAPK

    Plasmid
    #29690
    Purpose
    GFP reporter protein fused to three mutated GSK3 phosphorylation sites
    Depositor
    Edward De Robertis
    Article
    Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.
    Insert
    GFP glycogen synthase kinase 3 recognition site mutated primed by MAPK
    Use
    Tags
    Flag and Flag-EGFP
    Expression
    Mammalian
    Mutation
    recognition sites only, and E3 ligase site, GSK3 …
    Promoter
    SP6
    Available since
    Jan. 6, 2012
    Availability
    Academic Institutions and Nonprofits only
    29690-plasmid-map-sequence-id-129831

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  8. CHRM2-DuET

    Plasmid
    #213208
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CHRM2 (CHRM2 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213208-plasmid-map-sequence-id-430770

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  9. pCAGGs-RFP

    Plasmid
    #127347
    Purpose
    Expresses RFP constitutively via the CAGGS promoter in mammalian cells
    Depositor
    Phil Sharp
    Insert
    Red Fluorescent Protein
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAGGS Promoter
    Available since
    Dec. 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    127347-plasmid-map-sequence-id-246568

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  16. pG1C

    Plasmid
    #71740
    Purpose
    Modular shuttle vector for Geobacillus and E. coli
    Depositor
    Tom Ellis
    Article
    Reeve et al ACS Synth Biol. 2016 Jul 1.
    Type
    Empty backbone
    Use
    Synthetic Biology; Shuttle vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71740-plasmid-map-sequence-id-130141

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  21. pAK_DR30_CasRx_mCherry

    Plasmid
    #134844
    Purpose
    All in one overexpression of CasRx and guideRNA
    Depositor
    Michael Yaffe
    Insert
    RfxCas13d
    Use
    CRISPR
    Tags
    T2A mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Nov. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    134844-plasmid-map-sequence-id-266704

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  22. CIRTS-control

    Plasmid
    #213788
    Purpose
    CIRTS RNA targeting system for targeted knockdown in neurons. CIRTS-Calm3 and scramble control guide RNA is expressed under human Syn1 and U6 promoter, respectively.
    Depositor
    Timothy Bredy
    Article
    Liau et al Nat Commun. 2023 Nov 22;14(1):7616. doi:
    Insert
    CIRTS-Calm3-U6-control gRNA
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Syn1, U6
    Available since
    Feb. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    213788-plasmid-map-sequence-id-424349

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  28. Yeast Secrete and Detect

    Plasmid Kit
    #1000000166
    Purpose
    Yeast Golden Gate cloning for protein secretion in Saccharomyces cerevisiae and Pichia pastoris. Methanol utilization (MUT) promoters, signal peptides, C-terminal tags, episomal and integrative.
    Depositor
    Sylvestre Marillonnet
    Available since
    Jan. 28, 2021
    Availability
    Academic Institutions and Nonprofits only

  30. pIRES-EGFP-puro

    Plasmid
    #45567
    Depositor
    Michael McVoy
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    IRES-EGFP-Puro
    Expression
    Mammalian
    Mutation
    Promoter
    Phcmv (strong human cytomegalovirus immediate ear…
    Available since
    June 14, 2013
    Availability
    Academic Institutions and Nonprofits only
    45567-plasmid-map-sequence-id-237092

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Showing: 8191 - 8220 of 160955 results