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CrBlue-mcherry - pcDNA3.1

Plasmid

#64204

Purpose

CrBlue fused to mcherry on the C terminus

Depositor

Narasimhan Gautam

Article

Karunarathne et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

Insert

CrBlue

Tags

mcherry

Expression

Mammalian

Promoter

CMV

Available Since

April 13, 2015

Availability

Academic Institutions and Nonprofits only
vYM012_phiC31-nTRE-Ins(high)-pEF1s(high)-H2b-Citine

Plasmid

#197680

Purpose

"Insert(high)-pEF1s(high)" reporter plasmid

Depositor

Michael Elowitz

Article

Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

Insert

pEF1s-H2B-Citrine

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-RhoH

Plasmid

#23230

Depositor

Channing Der

Article

Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-

Insert

RhoH (RHOH Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only
pI6-MTU-DO-URA

Plasmid

#160030

Purpose

Integrative vector for K. marxianus, targeting the ARO3 locus. Uracil selection. Low integration efficiency in an NHEJ-deficient background.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3 NHE-RF

Plasmid

#11635

Depositor

Vijaya Ramesh

Article

Gonzalez-Agosti et al J Biol Chem. 1999 Nov 26. 274(48):34438-

Insert

NHE-RF (NHERF1 Human)

Tags

HA

Expression

Mammalian

Available Since

May 3, 2006

Availability

Academic Institutions and Nonprofits only
pDM1209

Plasmid

#108986

Purpose

extrachromosomal expression vector, C-terminal GFP tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum expression vector

Tags

GFP

Available Since

July 19, 2018

Availability

Academic Institutions and Nonprofits only

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pIRESN2 GFP-uvr8at

Plasmid

#44969

Depositor

Thanos Halazonetis

Article

Crefcoeur et al Nat Commun. 2013;4:1779. doi: 10.1038/nc

Insert

GFP-UVR8 (UVR8 Synthetic, Mustard Weed)

Tags

GFP

Expression

Mammalian

Mutation

deleted Methionine 1

Promoter

pCMV IE

Available Since

June 6, 2013

Availability

Academic Institutions and Nonprofits only

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pmNFM

Plasmid

#83126

Purpose

Mouse neurofilament protein M cDNA mammalian expression construct

Depositor

Anthony Brown

Article

Yan et al Cell Motil Cytoskeleton. 2007 Apr;64(4):

Insert

mouse NFM (Nefm Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pMSXND1-hPPT1

Plasmid

#85817

Purpose

Expression of hPPT in mammalian cells

Depositor

Sandra Hofmann

Article

Lu et al Mol Genet Metab. 2010 Apr;99(4):374-8. d

Insert

human PPT1 (PPT1 Human)

Expression

Mammalian

Available Since

Jan. 13, 2017

Availability

Academic Institutions and Nonprofits only
pTRANS_233

Plasmid

#91122

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: WDV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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TNXXL-CTS20

Plasmid

#48342

Purpose

FRET reporter for Ca2+ inside primary cilia

Depositor

Takanari Inoue

Article

Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

Insert

CTS20

Tags

TN-XXL

Expression

Mammalian

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pFA6a-link-yomKeima-CaURA3

Plasmid

#44885

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mKeima with CaUra3 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomKeima

Expression

Yeast

Available Since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_PA-Cre-Y324F

Plasmid

#122961

Purpose

Catalytically inactive mutant of PA-Cre

Depositor

Moritoshi Sato

Article

Kawano et al Nat Chem Biol. 2016 Dec;12(12):1059-1064

Insert

pcDNA3.1_PA-Cre-Y324F

Use

Cre/Lox

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoGFPgamma-SpHis5

Plasmid

#44859

Purpose

Yeast tagging vector to create gene fusion with yeast optimized GFPgamma with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoGFPgamma

Expression

Yeast

Available Since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

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Cox8x2-YeNL_pcDNA3

Plasmid

#89517

Purpose

Yellow color variant of bright luminescent protein, targeted to the Mitochondria

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

YeNL

Tags

CoxVIII X2

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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pBabe NEO Venus

Plasmid

#98396

Purpose

Retroviral vector for constitutive Venus expression

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

Venus

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 15, 2017

Availability

Academic Institutions and Nonprofits only

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cavin-3-HA

Plasmid

#27711

Depositor

Ari Helenius

Article

Hayer et al Traffic. 2010 Mar . 11(3):361-82.

Insert

cavin-3 (CAVIN3 Human)

Tags

HA

Expression

Mammalian

Mutation

R8P

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 mPapaya1.0

Plasmid

#52304

Purpose

An engineered monomeric Zoanthus sp. yellow fluorescent protein called mpapaya

Depositor

Robert Campbell

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

fluorescent protein mpapaya

Expression

Mammalian

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
pFA6a-mTID-HA3-KanMX6

Plasmid

#227982

Purpose

To insert biotin ligase, miniTurboID, at the C-terminus of genes; yeast BioID, KanMX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

miniTurboID

Tags

HA tag

Expression

Yeast

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hAIM2-T7

Plasmid

#51538

Purpose

Express T7-tagged hAIM2 in mammalian cells

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Tags

T7

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

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pDM1207

Plasmid

#108984

Purpose

extrachromosomal expression vector, N-terminal GFP tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum expression vector

Tags

GFP

Available Since

July 19, 2018

Availability

Academic Institutions and Nonprofits only

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pPL5628_TEF1*-Cre_MET15

Plasmid

#60933

Purpose

Constitutive expression vector for Cre-recombinase containing MET15 selection marker.

Depositor

Robert Piper

Article

MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

Insert

Cre

Tags

Driven from a mutant TEF1 promoter with 0.16x act…

Expression

Yeast

Promoter

mutant TEF1

Available Since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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Rtn3c-mEmerald

Plasmid

#186963

Purpose

Express Rtn3c with C-terminus mEmerald

Depositor

Ke Xu

Article

Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

RTN3C (Rtn3 Mouse)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV Promoter

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pMito-iRFP713

Plasmid

#45465

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP713

Tags

human cytochrome C oxidase subunit VIII

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, Y193K, F198…

Promoter

CMV

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pBS-TarHom-Tol2-kanaR-cryaa-sfGFP

Plasmid

#74153

Purpose

BAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker sfGFP

Depositor

Holger Knaut

Article

Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

Insert

Tol2-kanaR-cryaa-sfGFP

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-T1ER

Plasmid

#47928

Purpose

T1ER is a calcium sensor targeted to ER

Depositor

Tobias Meyer

Article

Bandara et al Sci Signal. 2013 Jul 9;6(283):ra56. doi:

Insert

T1ER

Expression

Mammalian

Mutation

KDEL

Promoter

CMV

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only
APneg-SNAP25c

Plasmid

#168013

Purpose

negative selection PACE Accessory Plasmid

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

SNAP25-T7PAP

Expression

Bacterial

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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APneg-SNAP25b

Plasmid

#168012

Purpose

negative selection PACE Accessory Plasmid

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

SNAP25-T7PAP

Expression

Bacterial

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-lynD3cpV

Plasmid

#37472

Depositor

Amy Palmer, Roger Tsien

Article

Palmer et al Chem Biol. 2006 May;13(5):521-30.

Insert

D3cpv second generation cameleon (calcium sensor)

Tags

the myristoylation and palmitoyla- tion sequence …

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2012

Availability

Academic Institutions and Nonprofits only
Vh-HA-3xFRB

Plasmid

#20223

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

3xFRB (MTOR Human)

Tags

HA and Vh

Expression

Mammalian

Mutation

One copy of FRB has a T2098L mutation

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pCAS9-mCherry-ACTG1

Plasmid

#66943

Purpose

CRISPaint target selector ACTG1

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

gRNA ACTG1

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pF CAG mc2 IRES neo

Plasmid

#206232

Purpose

For expressing cloned exons as circular RNA.

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
mPapaya1-C1

Plasmid

#54722

Purpose

Localization: C1 Cloning Vector, Excitation: 530, Emission: 541

Depositor

Robert Campbell, Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Type

Empty backbone

Tags

mPapaya1

Expression

Mammalian

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only
pALS3-Ma-SUMO-sfGFP-WT

Plasmid

#212122

Purpose

Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.

Depositor

Ryan Mehl

Inserts

SUMO-sfGFP-WT
M. alvus Pyl-tRNA(6)

Tags

His6 and SUMO

Expression

Bacterial

Promoter

araC and lpp

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pEF1a-mRor2N620K

Plasmid

#22631

Depositor

Roel Nusse

Article

Mikels et al J Biol Chem. 2009 Oct 30;284(44):30167-7

Insert

Ror2 N620K (Ror2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Changed Asparagine 620 to Lysine

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
mCerulean3-Plastin-C-10

Plasmid

#55438

Purpose

Localization: Fimbrin/Cytoskeleton, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Plastin (PLS1 Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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cmv 16 E7 del DLYC

Plasmid

#13687

Depositor

Karl Munger

Article

Gonzalez et al J Virol. 2001 Aug . 75(16):7583-91.

Insert

HPV 16 E7 del DLYC (E7 Human)

Expression

Mammalian

Mutation

del aa 21-24

Available Since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
pMaCTag-P31

Plasmid

#120042

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with V5

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

V5

Use

Pcr template

Tags

V5

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pB_35S/mEGFP

Plasmid

#135320

Purpose

mEGFP expression regulated by the CaMV35S promoter, co-expressed BASTA resistance selectable marker (bar)

Depositor

Mathias Pribil

Article

Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

Insert

mEGFP

Expression

Plant

Promoter

Cauliflower mosaic virus 35S

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pOVC1_X_ScPH1-S

Plasmid

#131193

Purpose

Expresses ScPH1-S (Synechosystis PCC6803) preceded by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

ScPH1-S

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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