We narrowed to 9,463 results for: Pol;
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Plasmid#202814PurposeSaccharomyces cerevisiae vector for the expression of polycistronic genes. Expression controled by the auto-inducible yeast promoter PCK1. LEU2 selection.DepositorTypeEmpty backboneExpressionYeastPromoterScPCK1Available SinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only
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pHYX138
Plasmid#202815PurposeSaccharomyces cerevisiae vector for the expression of polycistronic genes. Expression controled by the auto-inducible yeast promoter ADH2. URA3 selection.DepositorTypeEmpty backboneExpressionYeastPromoterScADH2Available SinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
MF87_PB-EF1a-TET3G-P2A-ERT2-Gal4-BGHpA
Plasmid#176630PurposeIntegrated in CHO-K1 to make parental polyclonal lines for all other MultiFate cell linesDepositorInsertEF1a-TET3G-P2A-ERT2-Gal4-BGHpA
UseSynthetic BiologyAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
MTK4_002
Plasmid#123811PurposeEncodes SV40 polyadenylation signal as a Type 4 part to be used in the MTK systemDepositorInsertSV40 pA
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT3TS_Transposase
Plasmid#109768PurposeCodon optimised transposase sequence in an optimal vector for in vitro transcription. Contains the T3 promoter and synthetic polyA tract.DepositorInsertTol2 Transposase
UseZebrafish plasmidsAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
Col2.3deltaTK
Plasmid#114273PurposeConditional ablation of mature bone cells via ganciclovir exposure of cells expressing a truncated thymidine kinase.DepositorInsertCol2.3 promoter + deltaTK + BGH polyA
ExpressionMammalianPromoter2.3 kb rat type 1 collagenAvailable SinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS462
Plasmid#89065Purposehis7 integration plasmid with Z3EVpr:GFPDepositorInsertZ3EV promoter GFP-NLS leu1 C-terminal 300 nt
UseS. pombe his7 integration vectorPromoterZ3EVAvailable SinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS2_Renilla_luc-60A
Plasmid#200397PurposeA plasmid IVT template containing a Renilla luciferase CDS followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.DepositorInsertRenilla luciferase
ExpressionMammalianAvailable SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPLP2H-mCf
Plasmid#229985PurposeLIC vector for improved polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.DepositorTypeEmpty backboneTagsHis6ExpressionBacterialPromoterT7Available SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPLP2S-mCf
Plasmid#229986PurposeLIC vector for improved polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. Strep-tag II CP tag for Strep-Tactin AC purification.DepositorTypeEmpty backboneTagsStrep-tag IIExpressionBacterialPromoterT7Available SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
DCAF1:PBC020-D05:C246337
Plasmid#183974PurposeBaculovirus expression for structure determination. May not contain entire coding region of gene.DepositorInsertDCAF1:PBC020-D05:C246337 (DCAF1 Human)
UseBaculovirus expresssionTags6xHis-TEV and MHHHHHHSSGRENLYFQGPromoterPolyhedrinAvailable SinceMay 10, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1(+) prME (JEV) sdm2
Plasmid#52881Purposeexpression of Japanese Encephalitis Virus VLPs in mammalian cellsDepositorAvailable SinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSmart-EF1a-Nla-cas11
Plasmid#178882PurposeExpression of human codon-optimized NlaCas11 with N terminal NLS and HA tagDepositorInsertEF1a-NlaCas11c-bGH polyA
TagsHA and NLSExpressionMammalianPromoterEF1aAvailable SinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-dSa-VPR mini.-2X snRP1
Plasmid#99688PurposeExpresses dSa Cas9 fused to optimized combination of truncated activation domains with 34 nt dual snRP1 poly adenylation signalDepositorArticleInsertdCas9
UseAAVTagsVPR miniMutationdead Cas9Available SinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJEP205-AAV-TRE3G-GFP-pA
Plasmid#164789PurposeAAV vector backbone designed to express GFP from a TRE3G promoter. It also contains a poly-Adenylation Signal (pA)DepositorInsertGreen Fluorescent protein (GFP)
UseAAVAvailable SinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDctp-mSox10-Mp1
Plasmid#13513DepositorAvailable SinceDec. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pX330-NEAT1m_v1
Plasmid#97083PurposeEncodes an sgRNA that creates a DSB immediately downstream the poly(A) signal of human NEAT1_1 isoformDepositorInsertsgRNA NEAT1m_v1
UseCRISPRPromoterU6Available SinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRN3P_T3_ABEmax_IVT
Plasmid#171761PurposePlasmid to be used as DNA template for in-vitro RNA transcription of the ABEmax base editor (A to G) by T3 RNA polymeraseDepositorInsertABEmax
UseVector for in-vitro transcriptionPromoterT3 promoterAvailable SinceDec. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX335-NEAT1_IS_v1
Plasmid#97085PurposeEncodes an sgRNA that creates a SSB near the poly(A) site of human NEAT1_1 isoform.DepositorInsertsgRNA NEAT1_IS_v1
UseCRISPRPromoterU6Available SinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-V96 ELP
Plasmid#67853PurposeGFP Fluorescent Elastin-like Polypeptide(VPGVG) with 96 repeats containing Valine guest residuesDepositorInsertGFP fused to ELP (VPGVG) repeated 96 times
TagsGFP fused to N-terminal of ELPExpressionMammalianPromoterT7Available SinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOCC154
Plasmid#118902Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal TwinStrep, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
TagsTwinStrep, cleavable with 3C proteaseExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC7
Plasmid#118876Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
TagsHIS6, cleavable with 3C proteaseExpressionInsectPromoterpolHAvailable SinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
RNF168-Halo HRD
Plasmid#207080PurposeHomologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF168 locus.DepositorInsertHaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences
ExpressionMammalianPromoterNoneAvailable SinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX335-NEAT1_IS_v2
Plasmid#97086PurposeEncodes an sgRNA that creates a SSB near the poly(A) site of human NEAT1_1 isoform.DepositorInsertsgRNA NEAT1_IS_v2
UseCRISPRPromoterU6Available SinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR-PCBP1
Plasmid#16177DepositorInsertPoly(rC) binding protein 1 (PCBP1 Human)
ExpressionMammalianAvailable SinceJan. 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRN3P_T3_BE4_IVT
Plasmid#171762PurposePlasmid to be used as DNA template for in-vitro RNA transcription of the BE4 base editor (C to T) by T3 RNA polymeraseDepositorInsertBE4
UseVector for in-vitro transcriptionPromoterT3 promoterAvailable SinceDec. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOCC153
Plasmid#118901Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal TwinStrep, cleavable with 3CDepositorInsertNcoI-TwinStrep-3C-NotI-ccdB-AscI-stop-HindIII cassette
TagsTwinStrep, cleavable with 3C proteaseExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
Clathrin-LCa-ECFP
Plasmid#21742DepositorAvailable SinceAug. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
hPDI1-18-508_C400S_C56S-pFLAG-CMV1
Plasmid#31383DepositorInsertPDI1A (P4HB Human)
TagsFLAG M2ExpressionMammalianMutationC-terminal cysteines of both active sites mutated…Available SinceMay 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T3-hCasD10A-pA
Plasmid#51638PurposeExpresses Cas9-nickase in mammalian cells and zygotes.DepositorInsertCodon-optimized Cas9 D10A
UseCRISPRTagsFLAG, NLS, and polyAExpressionMammalianPromoterCAG promoterAvailable SinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only