We narrowed to 11,636 results for: Vars

1. pαH-S-GSAS

   Plasmid

   #164565

   Purpose

   SARS-CoV-2 Spike protein (S-GSAS variant)

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

   Insert

   Spike (S-GSAS variant)
   

   Tags

   2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

   Expression

   Mammalian

   Mutation

   Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. MS2_adRNA-MCP_ADAR2 DD_NES

   Plasmid

   #124706

   Purpose

   Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR2 DD_NES

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

   Insert

   MCP_ADAR2 DD_NES (RAB7A Human)
   

   Use

   AAV

   Tags

   NES

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV.CAG-FLEX.SF-iGluSnFR.A184S.mRuby3

   Plasmid

   #106204

   Purpose

   Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Insert

   SF-iGluSnFR.A184S
   

   Use

   AAV

   Mutation

   GltI: A184S + mRuby3 fusion

   Promoter

   CAG

   Available Since

   Feb. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. MS2_adRNA-MCP_ADAR1 DD_NES

   Plasmid

   #124630

   Purpose

   Expresses MS2_adRNA targeting the RAB7A transcript along with MCP_ADAR1 DD_NES

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

   Insert

   MCP_ADAR1 DD_NES (RAB7A Human)
   

   Use

   AAV

   Tags

   NES

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-RR-ABE8e-V106W

   Plasmid

   #193656

   Purpose

   Expression of base editor RR-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   dLbCas12a-TadA*8e-V106W, EGFP
   

   Expression

   Mammalian

   Mutation

   dLbCas12a-G532R/K595R, TadA*8e-V106W

   Promoter

   CMV

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT2-TetR-neoR

   Plasmid

   #153310

   Purpose

   Tet-repressor with neomycin selection marker, to be used together with pT2-CMV/TetO2-EYFP-GW

   Depositor

   Spyros Petrakis

   Article

   Laidou et al Redox Biol. 2020 Feb 11;32:101458. doi:

   Insert

   TetR
   

   Use

   Transposon

   Promoter

   SV40

   Available Since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHH0103_BCAR1-1/1

   Plasmid

   #91418

   Purpose

   Protein expression and purification of human SH3 domain construct BCAR1-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   BCAR1-1/1 (BCAR1 Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB-cT3G-cERP2-NR1H2

   Plasmid

   #192919

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of NR1H2

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   NR1H2 (NR1H2 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX VAMP7 (1-188)

   Plasmid

   #42315

   Depositor

   Thierry Galli

   Article

   Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

   Insert

   VAMP7 Cytoplasmic domain (1-181) (VAMP7 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Δ181-220

   Promoter

   Ptac

   Available Since

   March 12, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pHH0103_FYN-1/1

    Plasmid

    #91413

    Purpose

    Protein expression and purification of human SH3 domain construct FYN-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    FYN-1/1 (FYN Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV_hsyn_NES-his-CaMPARI2-F391W-L398V

    Plasmid

    #119723

    Purpose

    AAV vector expressing CaMPARI2_F391W-L398V (Kd = 174nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

    Depositor

    Thomas Oertner

    Article

    Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

    Inserts

    CaMPARI2
    CaMPARI2
    

    Use

    AAV

    Tags

    NES-his

    Expression

    Mammalian

    Mutation

    F391W, L398V

    Promoter

    hSyn1

    Available Since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDF-Az

    Plasmid

    #197101

    Purpose

    To express the azidophenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into azidophenylalanine

    Depositor

    Kensaku Sakamoto

    Article

    Yamaguchi et al Bioconjug Chem. 2016 Jan 20;27(1):198-20

    Insert

    TyrRS variant, amber suppressor tRNA, kanamycin resistance gene
    

    Expression

    Bacterial

    Mutation

    Thr32, Asn107, Pro158, Leu159, Gln162, Arg286 (Ty…

    Available Since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pαH-S-RRAR/D614G

    Plasmid

    #164570

    Purpose

    SARS-CoV-2 Spike protein with D614G mutation and Furin site restored (S-RRAR-D614G variant)

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

    Insert

    Spike (S-RRAR-D614G variant)
    

    Tags

    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

    Expression

    Mammalian

    Mutation

    D614G

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV.CAG-FLEX.SF-iGluSnFR.A184V.mRuby3

    Plasmid

    #106205

    Purpose

    Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-iGluSnFR.A184V
    

    Use

    AAV

    Mutation

    GltI: A184V + mRuby3 fusion

    Promoter

    CAG

    Available Since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLL5.0-Anillin shRNA

    Plasmid

    #187270

    Purpose

    Lentiviral expression of shRNA targeting Anillin

    Depositor

    Alpha Yap

    Article

    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

    Insert

    shRNA for Anillin (ANLN Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Promoter

    U6

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTwist-SARS-CoV-2 Δ18 B.1.351v3

    Plasmid

    #169464

    Purpose

    Encodes SARS-CoV-2 B.1.351v3 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV-2 B.1.351v3 Spike truncated to remove 18 amino acids (S )
    

    Expression

    Mammalian

    Available Since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-dCas12a-A3A-N57G

    Plasmid

    #193639

    Purpose

    Expression of base editor dCas12a-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLbCas12a-hA3A-N57G, EGFP
    

    Expression

    Mammalian

    Mutation

    hAPOBEC3A-N57G

    Promoter

    CMV

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-dCas12a-A3A-Y130F

    Plasmid

    #193640

    Purpose

    Expression of base editor dCas12a-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLbCas12a-hA3A-Y130F, EGFP
    

    Expression

    Mammalian

    Mutation

    hAPOBEC3A-Y130F

    Promoter

    CMV

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV.hSynap-FLEX.SF-iGluSnFR.A184V

    Plasmid

    #106181

    Purpose

    Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Has Service

    AAV1

    Insert

    SF-iGluSnFR.A184V
    

    Use

    AAV

    Mutation

    GltI: A184V

    Promoter

    hSynapsin

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. eSpCas9-plus

    Plasmid

    #126767

    Purpose

    Expression plasmid for human codon-optimized increased fidelity eSpCas9-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    eSpCas9-plus
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    K848A, R1060A; amino acids 1005-1013 replaced wit…

    Promoter

    CBh

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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