We narrowed to 4,976 results for: puc

1. pre-Ubc4 BrG pUC19

   Plasmid

   #111322

   Purpose

   for transcription to Ubc4 pre-mRNA

   Depositor

   Christine Guthrie

   Article

   Abelson et al Methods Enzymol. 2010;472:31-40. doi: 10

   Insert

   UBC4 (UBC4 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   intron has branch-site A to G mutation

   Promoter

   Available since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUC57 DYSF, alt exon 1, 17

   Plasmid

   #87893

   Purpose

   research of alternative isoforms of the protein dysferlin

   Depositor

   Jain Foundation

   Insert

   DYSF, alt exon 1, 17 (DYSF Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   alternative isoforms, see comments

   Promoter

   Available since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUC57 DYSF, alt exon 1a

   Plasmid

   #87902

   Purpose

   research of alternative isoforms of the protein dysferlin

   Depositor

   Jain Foundation

   Insert

   DYSF, alt exon 1a (DYSF Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   alternative isoforms, see comments

   Promoter

   Available since

   March 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUC57 DYSF, alt exon 1a, 17

   Plasmid

   #87900

   Purpose

   research of alternative isoforms of the protein dysferlin

   Depositor

   Jain Foundation

   Insert

   DYSF, alt exon 1a, 17 (DYSF Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   alternative isoforms, see comments

   Promoter

   Available since

   March 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUC57 DYSF, alt exon 1a, 5a

   Plasmid

   #87898

   Purpose

   research of alternative isoforms of the protein dysferlin

   Depositor

   Jain Foundation

   Insert

   DYSF, alt exon 1a, 5a (DYSF Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   alternative isoforms, see comments

   Promoter

   Available since

   March 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUC57 DYSF, alt exon 1a, 5a, 17

   Plasmid

   #87896

   Purpose

   research of alternative isoforms of the protein dysferlin

   Depositor

   Jain Foundation

   Insert

   DYSF, alt exon 1a, 5a, 17 (DYSF Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   alternative isoforms, see comments

   Promoter

   Available since

   March 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUC57 DYSF, alt exon 1, 5a

   Plasmid

   #87890

   Purpose

   research of alternative isoforms of the protein dysferlin

   Depositor

   Jain Foundation

   Insert

   DYSF, alt exon 1, 5a (DYSF Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   alternative isoforms, see comments

   Promoter

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUC57 DYSF, alt exon 1, 5a, 17

   Plasmid

   #87888

   Purpose

   research of alternative isoforms of the protein dysferlin

   Depositor

   Jain Foundation

   Insert

   DYSF, alt exon 1, 5a, 17 (DYSF Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   alternative isoforms, see comments

   Promoter

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUC19 gene 60 thru gap

   Plasmid

   #49160

   Purpose

   Template for generating gene 60 mRNA truncated two codons after coding gap

   Depositor

   Nils Walter

   Article

   Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

   Insert

   Bacteriophage T4 gene 60
   

   Use

   Tags

   Expression

   Mutation

   gene 60 mRNA truncated two codons after coding ga…

   Promoter

   T7

   Available since

   Jan. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUC19 gene 60 to gap + Cys

    Plasmid

    #49159

    Purpose

    Template for generating gene 60 mRNA, sequence 5' of coding gap + Cys

    Depositor

    Nils Walter

    Article

    Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

    Insert

    Bacteriophage T4 gene 600
    

    Use

    Tags

    Expression

    Mutation

    Coding gap and downstream sequence have been dele…

    Promoter

    T7

    Available since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. puC19 preACT1(del6) A259C

    Plasmid

    #111329

    Purpose

    T7 transcription

    Depositor

    Christine Guthrie

    Article

    Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

    Insert

    ACT1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    branch site A259C

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. puC19 preACT1(del6) C256A

    Plasmid

    #111331

    Purpose

    T7 transcription

    Depositor

    Christine Guthrie

    Article

    Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

    Insert

    ACT1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    branch site C256A

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pIG-747pUC19-tNGFR-P2A-CD7

    Plasmid

    #186086

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CD7 (CD7 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUC18T-mini-Tn7T-hph-Ptet2

    Plasmid

    #218368

    Purpose

    Empty backbone for Tn7-site insertion with tetR-PtetA expression system with two tetR operator sites

    Depositor

    Michael Gebhardt

    Article

    Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUC18T-mini-Tn7T-hph-Ptet1

    Plasmid

    #218367

    Purpose

    Empty backbone for Tn7-site insertion with tetR-PtetA expression system with single tetR operator site

    Depositor

    Michael Gebhardt

    Article

    Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUC19-eGFP-P2A-dTAG-Ing3

    Plasmid

    #170754

    Purpose

    Donor plasmid for eGFP-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Ing3 locus

    Depositor

    Kristian Helin

    Article

    Damhofer et al STAR Protoc. 2021 Jun 2;2(2):100575. doi

    Insert

    eGFP-P2A-2xHA-dTAG
    

    Use

    Mouse Targeting

    Tags

    eGFP-P2A-2xHA-dTAG

    Expression

    Mutation

    Phenylalanine 36 to valine in FKBP12

    Promoter

    none

    Available since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUC CBA-SpCas9.EF1a-BFP.sgLMNA

    Plasmid

    #98971

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9 and a sgRNA targeting LMNA. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNA and spCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUC19-dual-scafffold-2.0 (pBP49)

    Plasmid

    #218931

    Purpose

    The dual-scaffold for making dual-CRISPR screening libraries or dual-CRISPR plasmids.

    Depositor

    Baoxu Pang

    Article

    Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

    Insert

    dual scaffolds for CRISPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJM220 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD)

    Plasmid

    #110559

    Purpose

    miniTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter

    Depositor

    Joanna Goldberg

    Article

    Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

    Insert

    rhaSR-PrhaBAD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rhaSR-PrhaBAD

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUC-DEST-hPROX1-tdTomato-PuroTK

    Plasmid

    #141027

    Purpose

    Reporter integration

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pUC18T-mini-Tn7T-hph-dCas9

    Plasmid

    #218371

    Purpose

    Expression of dCas9 for CRISPR-interference

    Depositor

    Michael Gebhardt

    Article

    Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PtetA

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pJM101(pUC18T-miniTn7T-gm-lacIq-Ptac)

    Plasmid

    #110558

    Purpose

    miniTn7 delivery plasmid with lacIq-Ptac inducible promoter

    Depositor

    Joanna Goldberg

    Article

    Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

    Insert

    lacIq-Ptac
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacIq-Ptac

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pUC18T-mini-Tn7T-Zeo-gfpmut3

    Plasmid

    #65037

    Purpose

    ZeoR mini-Tn7 vector, mobilizable, for GFP tagging bacteria

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Insert

    gfpmut3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pPW3754 : pUC-SpCas9_gRNA-HsIRE1-Cterm

    Plasmid

    #185676

    Purpose

    SpCas9 and gRNA targeting the C-terminus of HsIRE1

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERN1 gRNA (ERN1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

25. pUC19-TgKI-MCS-tdTomato-polyA

    Plasmid

    #174022

    Purpose

    This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

    Depositor

    Michel Bagnat

    Article

    Levic et al Development. 2021 Sep 8. pii: 272090. do

    Insert

    tdTomato
    

    Use

    Zebrafish knock-in tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pUC18T-mini-Tn7T-hph-Ptac

    Plasmid

    #218364

    Purpose

    Empty backbone for Tn7-site insertion with lacIq-Ptac expression system

    Depositor

    Michael Gebhardt

    Article

    Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pKB33 (pUC19-KANMX-tetO7-CYCpr)

    Plasmid

    #210472

    Purpose

    Plasmid with KanMX-tetO7-CYCpr cassette from the yTHC collection. Used for amplifying cassette with homology to regions of interest for genomic integration.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

    Insert

    KanMX-TetO7-CYCpr cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pUC18T-mini-Tn7T-Zeo-dsRedExpress

    Plasmid

    #65040

    Purpose

    ZeoR mini-Tn7 vector, mobilizable, for RFP tagging bacteria

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Insert

    dsRedExpress
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pIG-730pUC19-tNGFR-P2A-Coronin1A

    Plasmid

    #186110

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CORONIN1A
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pUC19 EGFRup-CD3ε-mNeonGreen-EGFRdown

    Plasmid

    #188634

    Purpose

    HDR template for CD3ε-mNeonGreen knock-in at C-terminus of human EGFR

    Depositor

    Jared Toettcher

    Article

    Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

    Insert

    EGFR (EGFR Synthetic, Human)
    

    Use

    CRISPR

    Tags

    CD3ε-mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge