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Plasmid#212753PurposeDoxycycline-inducible expression of NUbo-E3(63)-VSV in budding yeastDepositorInsertE3(63)
UseIntegrative vectorTagsNUbo and VSVExpressionYeastMutationPromoterTetAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-TET-NUbo-E3(48)-VSV
Plasmid#212744PurposeDoxycycline-inducible expression of NUbo-E3(48)-VSV in budding yeastDepositorInsertE3(48)
UseIntegrative vectorTagsNUbo and VSVExpressionYeastMutationPromoterTetAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-TET-NUbo-E3(48)-VSV
Plasmid#212743PurposeDoxycycline-inducible expression of NUbo-E3(48)-VSV in budding yeastDepositorInsertE3(48)
UseIntegrative vectorTagsNUbo and VSVExpressionYeastMutationPromoterTetAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-NUbo-E3(63)-FLAG
Plasmid#212808PurposeConstitutive or doxycycline-inducible expression of NUbo-E3(63)-FLAG in mammalian cellsDepositorInsertE3(63)
UseTagsFLAG and NUboExpressionMammalianMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-NUbo-FLAG
Plasmid#212797PurposeConstitutive/Doxycycline-inducible expression of NUbo-FLAG in mammalian cellsDepositorInsertNUbo
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCaUra3-CUbo(K48R/K63R)-yeGFP
Plasmid#212774PurposeC-terminal PCR-based tagging with CUbo(K48R/K63R)-yeGFP in budding yeast with CaUra3 selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHyg-CUbo(K48R)-yeGFP-His6
Plasmid#212783PurposeC-terminal PCR-based tagging with CUbo(K48R)-yeGFP-His6 in budding yeast with HygR selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHyg-CUbo(K63R)-yeGFP-His6
Plasmid#212784PurposeC-terminal PCR-based tagging with CUbo(K63R)-yeGFP-His6 in budding yeast with HygR selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHyg-CUbo(K48R/K63R)-yeGFP-His6
Plasmid#212785PurposeC-terminal PCR-based tagging with CUbo(K48R/K63R)-yeGFP-His6 in budding yeast with HygR selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHyg-CUbo(K48R/K63R)-9myc
Plasmid#212789PurposeC-terminal PCR-based tagging with CUbo(K48R/K63R)-9myc in budding yeast with HygR selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUra3-pCUP1-CUbo(K48R/K63R)
Plasmid#212765PurposeN-terminal PCR-based tagging with pCUP1-CUbo(K48R/K63R) in budding yeast with URA3 selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-CUP1-NUbo-E3(63)-VSV
Plasmid#212754PurposeCopper-inducible expression of NUbo-E3(63)-VSV in budding yeastDepositorInsertE3(63)
UseIntegrative vectorTagsNUbo and VSVExpressionYeastMutationPromoterCUP1Available sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-ADH-NUbo-E3(63)-VSV
Plasmid#212751PurposeConstitutive expression of NUbo-E3(63)-VSV in budding yeastDepositorInsertE3(63)
UseIntegrative vectorTagsNUbo and VSVExpressionYeastMutationPromoterADH1Available sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-ADH-NUbo-E3(63)-VSV
Plasmid#212750PurposeConstitutive expression of NUbo-E3(63)-VSV in budding yeastDepositorInsertE3(63)
UseIntegrative vectorTagsNUbo and VSVExpressionYeastMutationPromoterADH1Available sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-ADH-NUbo-E3(48)-VSV
Plasmid#212742PurposeConsitutive expression of NUbo-E3(48)-VSV in budding yeastDepositorInsertE3(48)
UseIntegrative vectorTagsNUbo and VSVExpressionYeastMutationPromoterADH1Available sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-ADH-NUbo-E3(48)-VSV
Plasmid#212741PurposeConsitutive expression of NUbo-E3(48)-VSV in budding yeastDepositorInsertE3(48)
UseIntegrative vectorTagsVSVExpressionYeastMutationPromoterADH1Available sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-ADH-CUbo-VSV
Plasmid#212733PurposeConsitutive expression of CUbo-VSV in budding yeastDepositorInsertCUbo
UseIntegrative vectorTagsVSVExpressionYeastMutationPromoterADH1Available sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-ADH-CUbo-VSV
Plasmid#212732PurposeConsitutive expression of CUbo-VSV in budding yeastDepositorInsertCUbo
UseIntegrative vectorTagsVSVExpressionYeastMutationPromoterADH1Available sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-CUP1-NUbo-VSV
Plasmid#212730PurposeCopper-inducible expression of NUbo-VSV in budding yeastDepositorInsertNUbo
UseIntegrative vectorTagsVSVExpressionYeastMutationPromoterCUP1Available sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-ADH-NUbo-VSV
Plasmid#212729PurposeConsitutive expression of NUbo-VSV in budding yeastDepositorInsertNUbo
UseIntegrative vectorTagsVSVExpressionYeastMutationPromoterADH1Available sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-ADH-NUbo-VSV
Plasmid#212728PurposeConsitutive expression of NUbo-VSV in budding yeastDepositorInsertNUbo
UseIntegrative vectorTagsVSVExpressionYeastMutationPromoterADH1Available sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-NUbo-E3(48)-FLAG
Plasmid#212802PurposeConstitutive/Doxycycline-inducible expression of NUbo-E3(48)-FLAG in mammalian cellsDepositorInsertE3(48)
UseTagsFLAG and NUboExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST-3xFLAG-Ubc7
Plasmid#212801PurposeConstitutive expression of 3xFLAG-tagged budding yeast Ubc7 in mammalian cellsDepositorInsertUbc7
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Myc-E3(1)-CUbo
Plasmid#212799PurposeConstitutive/Doxycycline-inducible expression of Myc-E3(1)-CUbo in mammalian cellsDepositorInsertE3(1)
UseTagsCUbo and MycExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Ubc13
Plasmid#212806PurposeConstitutive or doxycycline-inducible expression of budding yeast Ubc13 in mammalian cellsDepositorInsertUbc13
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Mms2
Plasmid#212807PurposeConstitutive or doxycycline-inducible expression of budding yeast Mms2 in mammalian cellsDepositorInsertMms2
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-His6-CUbo(K63R)-MCS
Plasmid#212727PurposeBacterial expression of a protein of interest with N-terminal His6-CUbo(K63R)-fusion, contains multiple cloning siteDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-His6-CUbo(K48R)-MCS
Plasmid#212726PurposeBacterial expression of a protein of interest with N-terminal His6-CUbo(K48R)-fusion, contains multiple cloning siteDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET15b-His6-CUbo(K63R)-GFP
Plasmid#212722PurposeBacterial expression of His6-CUbo(K63R)-GFPDepositorInsertGFP
UseTags6xHis-CUbo(K63R)ExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET15b-His6-CUbo(K48R)-GFP
Plasmid#212721PurposeBacterial expression of His6-CUbo(K48R)-GFPDepositorInsertGFP
UseTags6xHis-CUbo(K48R)ExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET15b-His6-CUbo-GFP
Plasmid#212720PurposeBacterial expression of His6-CUbo-GFPDepositorInsertGFP
UseTags6xHis-CUboExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-His7-NUbo-GFP
Plasmid#212719PurposeBacterial expression of His7-NUbo-GFPDepositorInsertGFP
UseTags7xHis-NUboExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-NUbo-GFP-His6
Plasmid#212718PurposeBacterial expression of NUbo-GFP-His6DepositorInsertGFP
UseTags6xHis and NUboExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET30-His6-TEV-Mms2
Plasmid#212716PurposeBacterial expression of His6-Mms2, His6-tag cleavable with TEV-proteaseDepositorInsertMms2
UseTags6xHis-TEVExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAL-TEV2-Ubc7-His6
Plasmid#212713PurposeBacterial expression of MBP-Ubc7-His6, MBP-tag cleavable with TEV-proteaseDepositorInsertUbc7
UseTags6xHis and MBP-TEV2ExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
FB398
Plasmid#203634PurposeModule for the expression of geneticin resistance, Nluc under PgpdA promoter and luciferase under a synthetic promoter containing the target sequence for gRNA1 (1xLuc).DepositorInsertFB367+FB395
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
FB323
Plasmid#203570PurposeModule for the expression of geneticin resistance and Nanoluciferase, and the expression of firefly luciferase under the Ppaf promoterDepositorInsertPgpdA:Nluc:Ttub::PtrpC:nptII:Ttub::Ppaf:Luc:Ttub
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_vhhGFP4::Nrv1::tagBFP
Plasmid#163925PurposeExpression of GrabFP-B-extracellular under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 (extracellular) nanobody with Nrv1 protein and tagBFP fluorophor
UseCre/LoxTagstagBFPExpressionInsectMutationPromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTW927
Plasmid#115961PurposeORF insert for replicon MoCloDepositorInsertLvl0 ORF TetR-2A-mKate-PEST GG
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTW099
Plasmid#115942PurposeSGP insert for replicon MoCloDepositorInsertLvl0 -98/14 SGP 2x K-turn
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJB557
Plasmid#115967PurposeSGP insert for replicon MoCloDepositorInsertLvl0 -98/16 SGP 2xTetApt-STOP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJB554
Plasmid#115964PurposeSGP insert for replicon MoCloDepositorInsertLvl0 -98/30 SGP 2xTetApt-STOP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTW101
Plasmid#115944PurposeSGP insert for replicon MoCloDepositorInsertLvl0 -98/16 SGP 2x K-turn
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTW098
Plasmid#115941PurposeSGP insert for replicon MoCloDepositorInsertLvl0 -98/5 SGP 2x K-turn
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTW097
Plasmid#115940PurposeSGP insert for replicon MoCloDepositorInsertLvl0 -98/3 SGP 2x K-turn
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTW095
Plasmid#115938PurposeSGP insert for replicon MoCloDepositorInsertLvl0 -98/16 SGP NA (No Aptamer)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTW084
Plasmid#115932PurposeSGP insert for replicon MoCloDepositorInsertLvl0 -98/15 SGP NA (No Aptamer)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJB553
Plasmid#115963PurposeSGP insert for replicon MoCloDepositorInsertLvl0 -98/5 SGP 1xTetApt-STOP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJB555
Plasmid#115965PurposeSGP insert for replicon MoCloDepositorInsertLvl0 -98/5 SGP 2xTetApt-STOP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTW096
Plasmid#115939PurposeSGP insert for replicon MoCloDepositorInsertLvl0 -98/30 SGP 2x K-turn
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only