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psgRNA-2xMS2-lacO

Plasmid

#181909

Purpose

Single guide RNA with 2XMS2 loops targeting bacterial lac operator

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

sgRNA-2XMS2-lacO

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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psgRNA-2xMS2-mSAT

Plasmid

#181908

Purpose

Single guide RNA with 2XMS2 loops targeting mouse major satellite repeats

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

sgRNA-2XMS2-mSAT

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pnCS_SEPT7_SEPT9_i1-i5-TEV-Strep

Plasmid

#180314

Purpose

bacterial co-expression of human SEPT7 and of human SEPT9_i1-i5

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Inserts

SEPTIN7 (SEPTIN7 Human)
SEPTIN9 (SEPTIN9 )

Tags

TEV-Strep

Expression

Bacterial

Mutation

SEPT9_i1-i5

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 S22A S23A-msfGFP

Plasmid

#180338

Purpose

mammalian expression of human SEPT9_i1 S22A S23A fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 S22A S23A

Promoter

CMV

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 S12A S13A-msfGFP

Plasmid

#180337

Purpose

mammalian expression of human SEPT9_i1 S12A S13A fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 S12A S13A

Promoter

CMV

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLgw NKX2-5-V5-EcoDam

Plasmid

#98597

Purpose

Mammalian DamID lentiviral vector for NKX2-5 with Dam-V5 using Gateway cloning

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

NKX2-5 (Nkx2-5 Mouse)

Use

Lentiviral; Damid

Tags

V5 and Dam (DNA adenine methyltransferase)

Expression

Mammalian

Promoter

Heat Shock Minimal Promoter

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCR8/GW/TOPO-ELK1Y158A

Plasmid

#98628

Purpose

Gateway entry TOPO TA cloning vector ELK1 Y158A

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

ELK1 Y158A (Elk1 Mouse)

Use

Topo ta cloning vector

Mutation

Y158A

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCR8/GW/TOPO-ETS1

Plasmid

#98621

Purpose

Gateway entry TOPO TA cloning vector for ETS1

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

ETS1 (Ets1 Mouse)

Use

Topo ta cloning vector

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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phDLX2-N174

Plasmid

#60860

Purpose

2nd generation lentiviral transfer plasmid. Expresses DLX2 under the EF1a promoter

Depositor

Andrew Yoo

Article

Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

Insert

DLX2 (DLX2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP SNAP23

Plasmid

#101914

Purpose

Expresses SNAP23 in mammalian cells

Depositor

Thierry Galli

Article

Kuster et al J Biol Chem. 2015 Nov 20;290(47):28056-6

Insert

SNAP 23 (SNAP23 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2+HyPer7

Plasmid

#136466

Purpose

Mammalian expression of non-targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Expression

Mammalian

Promoter

CMV, SP6

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-GFP-LaminA-IRES-Hygromycin

Plasmid

#134867

Purpose

Expresses EGFP-tagged nuclear envelope marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Lamin A (LMNA Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

EF1a

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pLifeAct-HyPer7

Plasmid

#136464

Purpose

Mammalian expression of F-actin targeted ultrasensitive hydrogen peroxide indicator HyPer7 fused with LifeAct peptide for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Tags

LifeAct

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-EGFP-AID-BubR1

Plasmid

#47330

Purpose

for plasmid integration using the Flp-In system. AID at N-terminal

Depositor

Don Cleveland

Article

Holland et al Proc Natl Acad Sci U S A. 2012 Dec 4;109

Insert

BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B Human)

Tags

AID and GFP

Expression

Mammalian

Mutation

siRNA resistant

Available Since

Sept. 3, 2013

Availability

Academic Institutions and Nonprofits only

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PB-TRE-dCas9-VPR

Plasmid

#63800

Purpose

SP-dCas9-VPR with doxycycline-inducible expression

Depositor

George Church

Article

Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

Insert

dCas9-VPR

Use

CRISPR and Synthetic Biology; Piggybac

Tags

VPR (VP64-p65-Rta)

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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EFIa-iTGB1

Plasmid

#115799

Purpose

Integrin B1 overexpression

Depositor

Joan Massague

Article

Er et al Nat Cell Biol. 2018 Aug;20(8):966-978. d

Insert

iTGB1 (ITGB1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-PTEN

Plasmid

#116866

Purpose

Recruitable PTEN dual-specificity phosphatase

Depositor

Gerry Hammond

Article

Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

Insert

PTEN (PTEN Human)

Tags

FKBP12 and mCherry

Expression

Mammalian

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV_hsyn_NES-his-CaMPARI2-F391W-L398V

Plasmid

#119723

Purpose

AAV vector expressing CaMPARI2_F391W-L398V (Kd = 174nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

Depositor

Thomas Oertner

Article

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Inserts

CaMPARI2
CaMPARI2

Use

AAV

Tags

NES-his

Expression

Mammalian

Mutation

F391W, L398V

Promoter

hSyn1

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV_hsyn_NES-his-CaMPARI2-H396K-WPRE-SV40

Plasmid

#101062

Purpose

AAV vector expressing CaMPARI2_H396K (Kd = 360nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

Depositor

Eric Schreiter

Article

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Insert

CaMPARI2-H396K

Use

AAV

Tags

FLAG-HA-myc and NES_his

Promoter

hsyn (synapsin-1)

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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TetON-shYAP

Plasmid

#115667

Purpose

Inducible YAP knockdown

Depositor

Joan Massague

Article

Er et al Nat Cell Biol. 2018 Aug;20(8):966-978. d

Insert

YAP shRNA (YAP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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