We narrowed to 10,311 results for: otos

Showing: 5821 - 5840 of 10311 results

Results per page:

Sort results by:

pMonAID_dCas9-PmCDA_Hyg_ALS

Plasmid

#91692

Purpose

Monocot Target-AID vector expressing rice-optimized dCas9-PmCDA1 with sgRNA targeting OsAls

Depositor

Akihiko Kondo

Article

Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

SpCas9

Tags

PmCDA1

Expression

Plant

Mutation

D10A and H840A for dead Cas9

Promoter

2x35S

Available Since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-ACBEmax

Plasmid

#139106

Purpose

Mammalian ACBEmax expression vector (pSI-632)

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-rAPOBEC1-2xUGI-SV40NLS

Use

CRISPR

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR KIF17 (1-738)

Plasmid

#60406

Purpose

Expression of truncated form of human homodimeric kinesin-2 (KIF17) amino acids 1-738

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

KIF17 (KIF17 Human)

Use

Lentiviral

Tags

GB1, Strep-Tag, and sfGFP

Expression

Mammalian

Mutation

Truncated version amino acids 1-738

Promoter

SFFV

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-ShadowY-CBD-P2A-Clover(T153M/F223R)-Cdc42

Plasmid

#165434

Purpose

Expresses ShadowY-CBD and Clover(T153M/F223R)-Cdc42 in mammalian cells to monitor Cdc42 activity with FLIM imaging.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

ShadowY-CBD-P2A-Clover(T153M/F223R)-Cdc42

Tags

ShadowY, Clover(T153M/F223R)

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p5E-hs_ICAM_full (JDW 1047)

Plasmid

#224479

Purpose

Gateway compatible 5' entry clone containing the Human ICAM2 promoter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

ICAM2 promoter

Expression

Mammalian

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTriEx-mCherry-Zdk1-RhoA Q63L

Plasmid

#81058

Purpose

mammalian expression of Zdk1 tagged RhoA Q63L

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Inserts

Zdk1
RhoA Q63L (RHOA Human)

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpCas9_BB_2A-GFP_MAPRE1-gRNA#1

Plasmid

#107726

Purpose

Knock out of EB1 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE1 gRNA #1 (targets Exon 1) (MAPRE1 Human)

Expression

Mammalian

Promoter

U6

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTYB11-PTBP1-RRM1

Plasmid

#89153

Purpose

Recombinant expression of PTBP1-RRM1 in E.coli

Depositor

Frédéric Allain

Article

Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

Insert

Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 1 (PTBP1 Human)

Tags

Intein and chitin binding domain

Expression

Bacterial

Promoter

T7

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
dmBACCS2NS-IRES-dOrai-IRES-GFP

Plasmid

#72895

Purpose

Mammalian expression of dmBACCS2NS, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFP

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

dmBACCS2NS-IRES-dOrai-IRES-GFP

Tags

IRES-GFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV_-CMV-Pcdh15_mini_V4-CD2

Plasmid

#199184

Purpose

An AAV plasmid designed to facilitate the expression of mini-PCDH15 in the inner ear of a mouse.

Depositor

David Corey

Article

Ivanchenko et al Nat Commun. 2023 Apr 26;14(1):2400. doi:

Insert

mmPcdh15_mini_V4-CD2

Use

AAV

Mutation

EC4, EC5, EC6, EC7, EC8 are deleted from msPCDH15…

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Lifect-7-pr-mEos2 (mEos2-A69T)

Plasmid

#99229

Purpose

Mammalian expression of the primed conversion capable protein pr-mEos2 (mEos2-A69T) as a Lifeact fusion.

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mEos2

Tags

Lifeact

Expression

Mammalian

Mutation

A69T for primed conversion applications

Promoter

CMV

Available Since

Oct. 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTriEx-mCherry-Zdk1-Rac1 Q61L

Plasmid

#81059

Purpose

mammalian expression of Zdk1 tagged Rac1 Q61L

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Inserts

Zdk1
Rac1 Q61L (RHOA Human)

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only
pTriEx-mVenus-Zdk1-RhoA Q63L

Plasmid

#81046

Purpose

mammalian expression of Zdk1 tagged RhoA Q63L

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Inserts

Zdk1
RhoA Q63L (RHOA Human)

Tags

mVenus

Expression

Bacterial, Insect, and Mamm…

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiPGK Puro DEST JNKKTR EE Clover

Plasmid

#90239

Purpose

Lentiviral vector to express JNK KTR EE (mutant) mClover under PGK promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

JNK Kinase Translocation Reporter (EE mutant)

Use

Lentiviral

Tags

mClover

Expression

Mammalian

Promoter

PGK

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTriEx-mVenus-Zdk1-Rac1 Q61L

Plasmid

#81137

Purpose

mammalian expression of Zdk1 tagged Rac1 Q61L

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Inserts

Zdk1
Rac1 (RHOA Human)

Tags

mVenus

Expression

Bacterial, Insect, and Mamm…

Mutation

Q61L

Available Since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
eSpCas9-EF-5Pou5f1

Plasmid

#174872

Purpose

CRISPR vector for generating Pou5f1 STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

sgRNA for mouse Pou5f1 (Pou5f1 Synthetic)

Use

CRISPR

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-human CSFI-R-L301S- Y969F-IRES-GFP

Plasmid

#133745

Purpose

retroviral expression of CSF1-R mutant

Depositor

Martine Roussel

Article

Roussel et al Nature. 1987 Feb 5-11;325(6104):549-52.

Insert

CSF1-R (CSF1R Human)

Use

Retroviral

Expression

Mammalian

Mutation

L301S- Y969F

Promoter

MSCV

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST-Tol2(Chien)-Krt4:Gal4VP16:tdTomato:5xUAS:HyPer:pA

Plasmid

#105643

Purpose

Co-expression of tdTomato and HyPer in differentiated keratinocytes of the periderm layer and ealy on also in the basal layer of the epidermis

Depositor

Sandra Rieger

Article

Lisse et al Proc Natl Acad Sci U S A. 2016 Apr 12;11

Insert

Krt4:Gal4VP16:tdTomato:5xUAS:HyPer:pA

Expression

Mammalian

Available Since

Feb. 2, 2018

Availability

Academic Institutions and Nonprofits only
hGATA-1 K137R

Plasmid

#118353

Purpose

This plasmid expresses human GATA-1 K137R mutant. This construct cannot undergo sumoylation on K137.

Depositor

Lea Sistonen

Article

Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

Insert

hGATA-1 (GATA1 Human)

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

K137R

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTAL_RFP

Plasmid

#43859

Depositor

Kiran Musunuru

Article

Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

Type

Empty backbone

Expression

Mammalian

Available Since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge