We narrowed to 11,985 results for: addgene/1000

1. GB1-A11(2-196, G175R)-Strep

   Plasmid

   #196211

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G175R)-Strep.

   Depositor

   Lalit Deshmukh

   Article

   Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

   Insert

   Annexin A11 (ANXA11 Human)
   

   Use

   Tags

   GB1-6xHis-TEV and Strep-II tag

   Expression

   Bacterial

   Mutation

   G175R

   Promoter

   Available since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. GB1-A11(2-196, G189E)-Strep

   Plasmid

   #196212

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G189E)-Strep.

   Depositor

   Lalit Deshmukh

   Article

   Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

   Insert

   Annexin A11 (ANXA11 Human)
   

   Use

   Tags

   GB1-6xHis-TEV and Strep-II tag

   Expression

   Bacterial

   Mutation

   G189E

   Promoter

   Available since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGBT9-ATG9A-N

   Plasmid

   #198530

   Purpose

   Expression of GAL4 DNA-binding domain (BD)-ATG9A-N terminal cytosolic domain fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al Proc Natl Acad Sci U S A. 2017 Dec 12;11

   Insert

   ATG9A N-terminal cytosolic tail (residues 1-66) (ATG9A Human)
   

   Use

   Tags

   GAL4-DNA binding domain fragment

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGBT9-FHIP-L

   Plasmid

   #198528

   Purpose

   Expression of GAL4 DNA-binding domain (BD)-FHIP-L fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

   Insert

   FHIP-L (FHIP1A Human)
   

   Use

   Tags

   GAL4-DNA binding domain fragment

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pnEA-NpM-HseIF4G_571-676-GB1_AG

   Plasmid

   #148745

   Purpose

   Bacterial Expression of HseIF4G_571-676

   Depositor

   Elisa Izaurralde

   Article

   Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

   Insert

   HseIF4G_571-676 (EIF4G1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pnEA-NpM-HseIF4G_592-653_AG

   Plasmid

   #148746

   Purpose

   Bacterial Expression of HseIF4G_592-653

   Depositor

   Elisa Izaurralde

   Article

   Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

   Insert

   HseIF4G_592-653 (EIF4G1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3_SP-HTR2A-NTEV-TCS-GV-2xHA

   Plasmid

   #194367

   Purpose

   Split TEV assays

   Depositor

   Michael Wehr

   Article

   Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

   Insert

   SP-HTR2A-NTEV-TCS-GV-2xHA (HTR2A Synthetic, Human)
   

   Use

   Tags

   2xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3_HTR2A-V2R-NTEV-TCS-GV-2xHA

   Plasmid

   #194368

   Purpose

   Split TEV assays

   Depositor

   Michael Wehr

   Article

   Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

   Insert

   HTR2A-V2R-NTEV-TCS-GV-2xHA (HTR2A Synthetic, Human)
   

   Use

   Tags

   2xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3_SP-HTR2A-V2R-NTEV-TCS-GV-2xHA

   Plasmid

   #194369

   Purpose

   Split TEV assays

   Depositor

   Michael Wehr

   Article

   Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

   Insert

   SP-HTR2A-V2R-NTEV-TCS-GV-2xHA (HTR2A Synthetic, Human)
   

   Use

   Tags

   2xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3_SP-DRD1-V2R-NTEV-TCS-GV-2xHA

    Plasmid

    #194361

    Purpose

    Split TEV assays

    Depositor

    Michael Wehr

    Article

    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

    Insert

    SP-DRD1-V2R-NTEV-TCS-GV-2xHA (DRD1 Synthetic, Human)
    

    Use

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pT7-EGFP-C1-HseIF4G1_615-654_AD

    Plasmid

    #148518

    Purpose

    Mammalian Expression of HseIF4G1_615-654

    Depositor

    Elisa Izaurralde

    Article

    Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

    Insert

    HseIF4G1_615-654 (EIF4G1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pT7-EGFP-C1-HseIF4G1_615-654-L640AV646A_AD

    Plasmid

    #148519

    Purpose

    Mammalian Expression of HseIF4G1_615-654-L640AV646A

    Depositor

    Elisa Izaurralde

    Article

    Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

    Insert

    HseIF4G1_615-654-L640AV646A (EIF4G1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pT7-EGFP-C1-HseIF4G_615-654-LIVVtoAAAA_AD

    Plasmid

    #148523

    Purpose

    Mammalian Expression of HseIF4G1_615-654-LIVVtoAAAA

    Depositor

    Elisa Izaurralde

    Article

    Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

    Insert

    HseIF4G1_615-654-LIVVtoAAAA (EIF4G1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pT7-EGFP-C1-HseIF4G_615-654-Y619AL624AL625A_AD

    Plasmid

    #148524

    Purpose

    Mammalian Expression of HseIF4G1_615-654-Y619AL624AL625A

    Depositor

    Elisa Izaurralde

    Article

    Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

    Insert

    HseIF4G1_615-654-Y619AL624AL625A (EIF4G1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAG-MBP-Foldon-ATG9 (801-839)

    Plasmid

    #188089

    Purpose

    To make ATG9 C-terminal tail trimer for expression in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG9 (ATG9A Human)
    

    Use

    Tags

    MBP tag

    Expression

    Mammalian

    Mutation

    ATG9 (801-839)

    Promoter

    CMV

    Available since

    Aug. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG-MBP-Foldon-ATG9 (723-839)

    Plasmid

    #188088

    Purpose

    To make ATG9 C-terminal tail trimer for expression in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG9 (ATG9A Human)
    

    Use

    Tags

    MBP tag

    Expression

    Mammalian

    Mutation

    ATG9 (723-839)

    Promoter

    CMV

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAG-MBP-Foldon-ATG9 (828-839)

    Plasmid

    #188090

    Purpose

    To make ATG9 C-terminal tail trimer for expression in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG9 (ATG9A Human)
    

    Use

    Tags

    MBP tag

    Expression

    Mammalian

    Mutation

    ATG9 (828-839)

    Promoter

    CMV

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pnYC-HseIF4E_AF

    Plasmid

    #148669

    Purpose

    Bacterial Expression of HseIF4E

    Depositor

    Elisa Izaurralde

    Article

    Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

    Insert

    HseIF4E (EIF4E Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    one silent mutation compared to the sequence of i…

    Promoter

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. H2A-mTurquoise-CDC42-G12V-deltaCAAX

    Plasmid

    #176094

    Purpose

    Constitutively active, nuclear localized Cdc42

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

    Insert

    CDC42 (CDC42 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G12V

    Promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHH0103_LRP5_Ldl-recept-b

    Plasmid

    #109867

    Purpose

    Protein expression and purification of LRP5_Ldl-recept-b

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    LRP5_Ldl-recept-b (LRP5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge