We narrowed to 1,243 results for: TIR

1. mCherry-p62-TIR-AAA

   Plasmid

   #187901

   Purpose

   Mammalian cell expression of a triple mutant of p62: P228A, E230A, P232A

   Depositor

   Erika Holzbaur

   Article

   Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

   Insert

   SQSTM1 (SQSTM1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Triple alanine mutant: P228A, E230A, P232A

   Promoter

   CMV

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET15b T7pCONS TIR-2 sfGFP

   Plasmid

   #154468

   Purpose

   pET15b with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

   Depositor

   Daniel Daley

   Article

   Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

   Insert

   super folder GFP
   

   Use

   Tags

   His-thrombin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-hSyn-OsTIR1(F74A)

   Plasmid

   #140731

   Purpose

   OsTIR1(F74A)-P2A-mAID-EGFP-NES

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   OsTIR1(F74A)-P2A-mAID-EGFP-NES
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSBbi-TIR1-tTA/Pur

   Plasmid

   #171683

   Purpose

   constitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (puromycin)

   Depositor

   Randy Poon

   Article

   Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

   Inserts

   tetracycline-controlled transactivator
   Puromycin
   EF-1a promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a promoter and RPBSA promoter

   Available since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPpT4- PGK1prom-TIR1-FLAG

   Plasmid

   #189724

   Purpose

   Enables expression of OsTIR1 from the PGK1 promoter, used to implement the AID system in Komagataella phaffii (original name pAEA454)

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

   Insert

   OsTIR1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PGK1 promoter

   Available since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a T7pCONS TIR-1 sfGFP

   Plasmid

   #154463

   Purpose

   pET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

   Depositor

   Daniel Daley

   Article

   Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

   Insert

   super folder GFP
   

   Use

   Tags

   His-thrombin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. C-SWAT AID*-GFP osTIR1

   Plasmid

   #182514

   Purpose

   C-SWAT compatible vector for C-terminal tagging with AID*-GFP for auxin inducible gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   AID*
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAN19-3xFLAG-ccvTIR1-NOSt

   Plasmid

   #108546

   Purpose

   Cloning vector including N-terminally 3xFLAG-tagged Arabidopsis auxin receptor TIR1 with the F79G mutation [ccvTIR1] and NOS terminator

   Depositor

   Keiko Torii

   Article

   Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d

   Insert

   TRANSPORT INHIBITOR RESPONSE 1 [F79G] fused with 3xFLAG and NOS terminator (TIR1 Mustard Weed)
   

   Use

   Cloning vector

   Tags

   3xFLAG

   Expression

   Mutation

   Changed F79 to G

   Promoter

   Available since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUCHR-osTIR1-IRES-GFP

   Plasmid

   #110661

   Purpose

   Lentiviral vector for stable expression of osTIR1 and selection with GFP

   Depositor

   Dmitriy Mazurov

   Article

   Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

   Insert

   osTIR1
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   March 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PBS Actb-2A-TIR1-GFP

    Plasmid

    #113832

    Purpose

    targeting osTIR1-GFP into mouse Actb locus for auxin inducible degradation

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    osTIR1-GFP (NEWENTRY Synthetic)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBAD/HisB (TIREVOL)-mEos3.2

    Plasmid

    #189720

    Purpose

    titratable expression of mEos3.2 in bacteria

    Depositor

    Daniel Daley

    Article

    Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

    Insert

    mEos3.2
    

    Use

    Tags

    His-T7-EK

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBAD/HisB (TIRSTD)-sfGFP

    Plasmid

    #189674

    Purpose

    titratable expression of sfGFP in bacteria

    Depositor

    Daniel Daley

    Article

    Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

    Insert

    sfGFP
    

    Use

    Tags

    His-T7-EK

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBAD/HisB (TIRSTD)-mNG

    Plasmid

    #189721

    Purpose

    titratable expression of mNeonGreen in bacteria

    Depositor

    Daniel Daley

    Article

    Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

    Insert

    mNeonGreen
    

    Use

    Tags

    His-T7-EK

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPpT4- TEF2prom-TIR1F74G-FLAG

    Plasmid

    #189727

    Purpose

    Enables expression of OsTIR1(F74G) from the TEF2 promoter, used to implement the AID2 system in Komagataella phaffii (original name pLB219)

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

    Insert

    OsTIR1
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    TEF2

    Available since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBAD/HisB (TIREVOL)-PAmCherry1

    Plasmid

    #189718

    Purpose

    titratable expression of PAmCherry1 in bacteria

    Depositor

    Daniel Daley

    Article

    Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

    Insert

    PAmCherry1
    

    Use

    Tags

    His-T7-EK

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSBbi-TIR1-tTA/Neo

    Plasmid

    #171684

    Purpose

    constitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (G418)

    Depositor

    Randy Poon

    Article

    Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

    Insert

    Neomycin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RPBSA promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAGGS-PB1-MontiRed-LOV2

    Plasmid

    #178872

    Purpose

    Mammalian expression of PB1-MontiRed-LOV2

    Depositor

    Shinya Tsukiji

    Article

    Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

    Insert

    PB1-MontiRed-LOV2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAGGS-PB1-MontiRed-LOV2dark

    Plasmid

    #178874

    Purpose

    Mammalian expression of PB1-MontiRed-LOV2dark

    Depositor

    Shinya Tsukiji

    Article

    Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

    Insert

    PB1-MontiRed-LOV2dark
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    LOV2: C450A, L514K, G528A, L531E, N538E

    Promoter

    CAG

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAN19-3xFLAG-TIR1-NOSt

    Plasmid

    #108545

    Purpose

    Cloning vector including N-terminally 3xFLAG-tagged Arabidopsis auxin receptor TIR1 [Wild type] and NOS terminator

    Depositor

    Keiko Torii

    Article

    Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d

    Insert

    TRANSPORT INHIBITOR RESPONSE 1 fused with 3xFLAG and NOS terminator (TIR1 Mustard Weed)
    

    Use

    Cloning vector

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. KJ201: pMVP (R4-R3) osTir1 open

    Plasmid

    #121743

    Purpose

    pMVP R4-R3 entry plasmid, contains osTir1 (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    osTir1 (open)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    9x myc epitope tag

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAS_pb_Ef1a-TIR1_Ubc-mCherry-Puro

    Plasmid

    #195567

    Purpose

    PiggyBac plasmid for stable expression of TIR1, mCherry, and Puromycin resistance in mammalian cells

    Depositor

    Joanna Wysocka

    Article

    Spencley et al Mol Cell. 2023 May 18;83(10):1623-1639.e

    Insert

    TIR1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1-alpha

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pACYCDuet-AtTIR1-Myc-MBP-HA

    Plasmid

    #222709

    Purpose

    Expression AtTIR1-Myc and MBP-HA in in bacterial cells

    Depositor

    Lei Wu

    Article

    Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

    Insert

    AtTIR1 (TIR1 Mustard Weed)
    

    Use

    Tags

    myc

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. RepairTemplate_AAVS1-OsTIR1-F74G-SNAP

    Plasmid

    #217665

    Purpose

    Repair template to integrate OsTIR1-F74G-SNAP into the human AAVS1 safe harbour locus

    Depositor

    Daniel Gerlich

    Article

    Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

    Insert

    OsTir1 F74G-SNAP-Blast-AAVS1
    

    Use

    Tags

    SNAP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Cas9-T2A-H2B mCitirine

    Plasmid

    #210471

    Purpose

    Coexpression of Cas9 and H2B mCitrine

    Depositor

    Amro Hamdoun

    Insert

    Cas9-T2A-H2B mCitirine
    

    Use

    CRISPR and Synthetic Biology

    Tags

    mCitrine on C terminal of H2B

    Expression

    Mammalian

    Mutation

    Cas9 contains 3xFLAG tag and nuclear localization…

    Promoter

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBAD/HisB (TIRSTD)-mEos3.2

    Plasmid

    #189719

    Purpose

    titratable expression of mEos3.2 in bacteria

    Depositor

    Daniel Daley

    Article

    Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

    Insert

    mEos3.2
    

    Use

    Tags

    His-T7-EK

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD/HisB

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. C-SWAT AID*-6MYC osTIR1

    Plasmid

    #193320

    Purpose

    C-SWAT compatible vector for C-terminal tagging with AID*-6MYC for auxin induced gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration into the genome.

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Insert

    AID*
    

    Use

    Tags

    6MYC

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. C-SWAT AID*-6FLAG osTIR1

    Plasmid

    #182515

    Purpose

    C-SWAT compatible vector for C-terminal tagging with AID*-6FLAG for auxin induced gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration into the genome.

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Insert

    AID*
    

    Use

    Tags

    6FLAG

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBAD/HisB (TIR23)-sfGFP

    Plasmid

    #189676

    Purpose

    titratable expression of sfGFP in bacteria

    Depositor

    Daniel Daley

    Article

    Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

    Insert

    sfGFP
    

    Use

    Tags

    His-T7-EK

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pPpT4- HTA1prom-TIR1-FLAG

    Plasmid

    #189725

    Purpose

    Enables expression of OsTIR1 from the HTA1 promoter, used to implement the AID system in Komagataella phaffii (original name pLLpLL001)

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

    Insert

    OsTIR1
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    HTA1

    Available since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pPpT4- TEF2prom-TIR1-FLAG

    Plasmid

    #189726

    Purpose

    Enables expression of OsTIR1 from the TEF2 promoter, used to implement the AID system in Komagataella phaffii (original name pLL002)

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

    Insert

    OsTIR1
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    TEF2

    Available since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge