We narrowed to 5,504 results for: pcas

1. pCas-Cas12m

   Plasmid

   #192274

   Purpose

   Encodes MmCas12m under a constitutive promoter

   Depositor

   John van der Oost

   Article

   Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

   Insert

   MmCas12
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PJ23108

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCA-FLEX

   Plasmid

   #38041

   Depositor

   Naoshige Uchida

   Article

   Watabe-Uchida et al Neuron. 2012 Jun 7;74(5):858-73.

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CA

   Available since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCA-ZtTA

   Plasmid

   #26083

   Depositor

   Liqun Luo

   Article

   Li et al PLoS One. 2010 . 5(7):e11503.

   Insert

   a loxP-flanked β-geo preceding the tetracycline transactivator tTA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCas-mut4_1_2_VA

   Plasmid

   #186441

   Purpose

   Encodes Cas4 (K70A), Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   Cas4, Cas1 and Cas2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJSC270 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant

   Plasmid

   #101217

   Purpose

   Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant K848A/K1003A/R1060A/N497A/R661A/Q695A/Q926A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   K848A, K1003A, R1060A, N497A, R661A, Q695A and Q9…

   Promoter

   T7

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCASB

   Plasmid

   #190175

   Purpose

   Plasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequence

   Depositor

   Jessica Tyler

   Article

   Aguilar et al Methods Protoc. 2022 Oct 4;5(5):79. doi:

   Insert

   sgRNA cloning site
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Added 5'-agagacc-3' upstream and 5'…

   Promoter

   Available since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCas9CyMutL

   Plasmid

   #166981

   Purpose

   Encodes aTc-inducible spCas9 and cumate-inducible MutLE32K for transient suppression of mismatch repair

   Depositor

   Christopher Reisch

   Article

   Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

   Inserts

   MutL (mutL )
   CymR
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   E32K

   Promoter

   pCymRC and pLacI

   Available since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGFP

   Plasmid

   #32748

   Depositor

   Jeanne Hardy

   Article

   Nicholls et al J Biol Chem. 2011 Jul 15;286(28):24977-8

   Insert

   GFP
   

   Use

   Tags

   DEVDFQGPCNDSSDPLVVAASIIGILHLILWILDRL

   Expression

   Mammalian

   Mutation

   S65T

   Promoter

   CMV

   Available since

   March 5, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCas9

   Plasmid

   #42876

   Purpose

   Bacterial expression of Cas9 nuclease, tracrRNA and crRNA guide

   Depositor

   Luciano Marraffini

   Article

   Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

   Insert

   tracr/Cas9
   

   Use

   CRISPR; E.coli

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCas9

    Plasmid

    #167547

    Purpose

    Used for CRISPR-Cas9 mediated recomineering in Enterococcus. Can clone desired gRNA using BsaI digestion.

    Depositor

    Howard Hang

    Article

    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00

    Inserts

    chloramphenicol acetyl transferase
    pUC19 origin of replication
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRepCap6

    Plasmid

    #110770

    Purpose

    pRepCap6 contains both rep and cap genes from the AAV6 genome, but lacks the viral terminal repeats (Rutledge et al., 1998). It lacks adenovirus helper functions required for stock production.

    Depositor

    David Russell

    Article

    Rutledge et al J Virol. 1998 Jan;72(1):309-19.

    Insert

    AAV6 cap, AAV6 rep genes
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCasPA

    Plasmid

    #113347

    Purpose

    Bacterial expression of Cas9 nuclease and λ-Red system in Pseudomonas aeruginosa

    Depositor

    Quanjiang Ji

    Article

    Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAROTENE5X

    Plasmid

    #208816

    Purpose

    Encodes bacterial crtEBIY operon under the control of synthetic promoter responsive to JUB1-derived artificial transcription factor in bacterial cell

    Depositor

    Marc Erhardt

    Article

    Naseri et al Commun Biol. 2023 Oct 3;6(1):1005. doi:

    Insert

    Five copies of JUB1 binding site within the synthetic promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    JUB1 TF-responsive promoter

    Available since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGGS_L_MARV

    Plasmid

    #135473

    Purpose

    Mammalian expression vector encoding Marburg virus L gene under control of the AG promoter

    Depositor

    Elke Mühlberger

    Article

    Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4

    Insert

    L gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    silent mutation (C to T) at position 7861; insert…

    Promoter

    AG

    Available since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAGGS_VP35_MARV

    Plasmid

    #135471

    Purpose

    Encodes Marburg virus VP35 gene under control of the AG promoter

    Depositor

    Elke Mühlberger

    Article

    Schmidt et al J Infect Dis. 2011 Nov;204 Suppl 3:S861-

    Insert

    VP35
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    AG

    Available since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS_NP_MARV

    Plasmid

    #135470

    Purpose

    Encoding Marburg virus NP gene under control of the AG promoter

    Depositor

    Elke Mühlberger

    Article

    Schmidt et al J Infect Dis. 2011 Nov;204 Suppl 3:S861-

    Insert

    nucleoprotein (NP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    AG

    Available since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAGGS_VP30_MARV

    Plasmid

    #135472

    Purpose

    Mammalian expression vector encoding Marburg virus VP30 gene under control of the AG promoter

    Depositor

    Elke Mühlberger

    Article

    Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4

    Insert

    VP30
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    AG

    Available since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGGS_cAMPFIRE-L

    Plasmid

    #182283

    Purpose

    Mammalian expression of the cAMP sensor cAMPFIRE-L under a CAG promotor w/ WPRE translation enhancer.

    Depositor

    Haining Zhong

    Article

    Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

    Insert

    cAMPFIRE-L (cAMP sensor)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-oG

    Plasmid

    #74288

    Purpose

    To express oG (optimized Glycoprotein) under CAG promoter

    Depositor

    Edward Callaway

    Article

    Kim et al Cell Rep. 2016 Apr 26;15(4):692-699. doi

    Insert

    oG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    chimeric glycoprotein

    Promoter

    CAG

    Available since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCas3cRh

    Plasmid

    #133773

    Purpose

    Expresses all necessary components of Type I-C CRISPR-Cas system

    Depositor

    Joseph Bondy-Denomy

    Article

    Csorgo et al Nat Methods. 2020 Oct 19. pii: 10.1038/s

    Inserts

    RhaR (rhaR )
    RhaS (rhaS )
    pRhaBAD
    I-C crRNA
    Cas3
    Cas5
    Cas8
    Cas7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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