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GFP-mROCK1-delta5
Plasmid#101293PurposeMammalian expression of Rock1-GFP for the study of localisation signaling.DepositorInsertRock1 (Rock1 Mouse)
UseTagsExpressionMammalianMutation375-727 aaPromoterCMVAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_IGF2BP2b-Halo
Plasmid#175318PurposeProduction of HaloTag fusion proteinDepositorInsertIGF2BP2 (IGF2BP2 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationIsoform BPromoterAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCx55.5-TrpR
Plasmid#86373Purposefor zebrafish transgenesis, expression of TrpR from Cx55.5 promoter, also expresses mCherryDepositorInsertTrpR
UseZebrafishTagsExpressionMutationPromotercx55.5Available sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
JBEI-6823
Plasmid#84986PurposeFor expression of MevTaco-PMKco-MKco(aka 2B) under lacUV5 promoter for isopentenol productionDepositorInsertMevTaco-PMKco-Mkco
UseTagsExpressionBacterialMutationPromoterLacUV5Available sinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag 3A/myc-M1L195V
Plasmid#87716PurposeExpresses myc-M1L195V spastin isoform in mammalian cellsDepositorInsertSPAST (SPAST Human)
UseTagsMycExpressionMammalianMutationM1 isoform; L195VPromoterCMVAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRE/SP M1WT
Plasmid#89327PurposeInducible expression of WT M1 spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutation1-194 bp of 5'UTR deletedPromoterTet-responsive P tightAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRE/SP M87 WT
Plasmid#89328PurposeInducible expression of WT M87 spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutation1-460 bp deletedPromoterTet-responsive P tightAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
mKO-Golgi-7
Plasmid#57843PurposeLocalization: Golgi Complex, Excitation: 548, Emission: 561DepositorInsertGolgi (B4GALT1 Human)
UseTagsmKOExpressionMammalianMutationaa 1-82 of NM_001497.3PromoterCMVAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDD2002
Plasmid#101198PurposeName: pCBG68-LANApi. LANApi driving green luciferase reporter for CBG68 in pCBG68 backbone.DepositorInsertLANApi driving green luciferase reporter for CBG68 in pCBG68 backbone.
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDD2005
Plasmid#101219PurposeName: pCBR-K14. K14 driving red luciferase reporter for CBR in pCBR backbone.DepositorInsertK14 driving red luciferase reporter for CBR in pCBR backbone.
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSP571
Plasmid#139498PurposeCRISPR-Cas9 plasmid to generate double strand break in STL1 locus in S. cerevisiae. Expresses both Cas9 and STL1 sgRNADepositorInsertpGPD Cas9 / sgRNA (STL162)
UseTagsExpressionYeastMutationWTPromoterpGPDAvailable sinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot2
Plasmid#79255PurposeExpresses EGFP-tagged DmNot2 in S2 cellsDepositorInsertDmNot2 (Rga Fly)
UseSchneider 2 cell expressionTagsEGFPExpressionMutationPromoterAc5 promoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS cCollagen XII (CT#1051)
Plasmid#13916DepositorInsertCollagen XII (COL12A1 Chicken)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
Cdc42 T17N pCS2
Plasmid#16670DepositorInsertCdc42 T17N (CDC42 Human)
UseVertebrateTagsExpressionMutationT17N. Dominant negative.PromoterAvailable sinceApril 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag 3A/myc-M1S46X
Plasmid#87718PurposeExpresses myc-M1S46X spastin isoform in mammalian cellsDepositorInsertSPAST (SPAST Human)
UseTagsMycExpressionMammalianMutationM1 isoform; S46XPromoterCMVAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag 3A/myc-M1S44L
Plasmid#87717PurposeExpresses myc-M1S44L spastin isoform in mammalian cellsDepositorInsertSPAST (SPAST Human)
UseTagsMycExpressionMammalianMutationM1 isoform; S44LPromoterCMVAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
JBEI-6822
Plasmid#84985PurposeFor expression of pBbA5c-MevTaco-MKco-PMKco(aka 2A) under lacUV5 promoter for isopentenol productionDepositorInsertMevTaco-MKco-PMKco
UseTagsExpressionBacterialMutationPromoterLacUV5Available sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-M2-TLR2-FW/AA
Plasmid#12293DepositorInsertTLR2 (Tlr2 Mouse)
UseTagsFLAG (FLAG-M2)ExpressionMammalianMutationM82I, H202Y, F774A and W775A; signal peptide (aa1…PromoterAvailable sinceJan. 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLJC2-NADK-3xFLAG
Plasmid#233888Purposelentiviral overexpression vector for NADKDepositorInsertNADK (NADK Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLJC2-NADK (D184A)-3xFLAG
Plasmid#233889Purposelentiviral overexpression vector for NADK (D184A)DepositorInsertNADK (NADK Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationD184APromoterCMVAvailable sinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLJC2-Blast-Rap2A-3xFLAG
Plasmid#233890Purposelentiviral overexpression vector for RAP2ADepositorInsertRAP2A (RAP2A Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLJC2-Blast-SHMT2-3xFLAG
Plasmid#233891Purposelentiviral overexpression vector for SHMT2DepositorInsertSHMT2 (SHMT2 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLJC6-NADK (D184A)-3xFLAG
Plasmid#233894Purposelentiviral overexpression vector NADK (D184A)DepositorInsertNADK (NADK Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationD184APromoterUbcAvailable sinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZH61 pLIB-8His3C-spt4
Plasmid#231649Purposeexpress S.cerevisiae spt4 in insect cellsDepositorInsertspt4 (SPT4 Budding Yeast)
UseTags8His-HRV3CExpressionInsectMutationPromoterAvailable sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZH82 pET28a-Rai1
Plasmid#231651PurposeBacterial expression vector expressing yeast S. cerevisiae rai1, no tagDepositorInsertrai1 (RAI1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZH23 Dual G-less containing ySSA1 polyA
Plasmid#231647Purposeyeast S.cerevisiae SSA1 polyA termination region inserted between the dual G-less cassetteDepositorInsertSSA1 polyA termination region
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZH20 Dual G-less containing yCYC1 polyA
Plasmid#231646Purposeyeast S.cerevisiae CYC1 polyA termination region inserted between the dual G-less cassetteDepositorInsertCYC1 polyA termination region
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
Galt1-HT7-mGold
Plasmid#230027PurposeExpressing fluorescent HaloTag7 fusion protein in mammalian cells with localization in the Golgi apparatus.DepositorInsertGalt1-HaloTag7-mGold (B4GALT1 Synthetic, Human)
UseTagsHaloTag,mGoldExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
p104Tol2-tp1:Cas9-t2A-GFP, 4xU6:sgRNA
Plasmid#227773PurposeExpression of tp1 (Notch) dependent Cas9-GFP and U6-driven 4 sgRNAs in zebrafishDepositorInsertsCas9-t2A-GFP
4xU6:sgRNA
UseCRISPRTagsExpressionMutationPromoterU6 and tp1Available sinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
p155ME-loxP-dsR-loxP-Cas9-t2A-GFP
Plasmid#227774PurposeMiddle-entry vector with dsRed flanked by loxP sites and spCas9-GFP for Gateway cloningDepositorInsertloxP-dsR-loxP
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-A51P/A56P
Plasmid#216400PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56 to proline mutationsDepositorInsertEfg1-ΔDBD-A51P/A56P
UseTags6xHis-tagsExpressionBacterialMutationchanged Alanine 51 and 56 to Proline, and deleted…PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Q443P
Plasmid#216401PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Q443 to proline mutationDepositorInsertEfg1-ΔDBD-Q443P
UseTags6xHis-tagsExpressionBacterialMutationchanged Glutamine 443 to Proline, and deleted ami…PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mRAB14-HA
Plasmid#214165PurposeTransient expression of RAB14DepositorInsertRAB14 (Rab14 Mouse)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDY380
Plasmid#182962PurposeAmp-resistant, low copy (p15A ori), E. coli 5'ptsI transcriptionally fused with GFP.DepositorInsertptsI
UseSynthetic BiologyTagsGFPExpressionMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDY390
Plasmid#182963PurposeAmp-resistant, low copy (p15A ori), E. coli ptsI transcriptionally fused with GFP.DepositorInsertptsI
UseSynthetic BiologyTagsGFPExpressionMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pN1-TOMM20-Halo
Plasmid#129348PurposeHalo Tag, Mitochondrial outer membrane localizationDepositorInsertHalo (Promega)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBHRSF228
Plasmid#135007PurposeMusF2 prenyltransferases from Nostoc sp. UHCC 0398DepositorInsertMusF2 prenyltransferase
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pSP569
Plasmid#139499PurposeRepair DNA plasmid to insert PP7 stem loops in the endogenous STL1 locus after double strand break in S. cerevisiaeDepositorInsertpSTL1 24xPP7sl STL1 100 - 600
UseTagsExpressionYeastMutationWTPromoterpSTL1Available sinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits only
