We narrowed to 12,024 results for: pcdna

1. pcDNA 3.2/V5-DEST NET1A S46A

   Plasmid

   #69818

   Purpose

   Expresses NET1A-V5 in mammalian cells

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   Neuroepithelial cell-transforming gene 1 protein A (NET1 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   S46A

   Promoter

   CMV

   Available Since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO/Flag-tGFP-Cryz

   Plasmid

   #110378

   Purpose

   Mammalian expression of turboGFP-CRYZ with FLAG tag, Flp-In T-Rex system

   Depositor

   Sandra Martha Gomes Dias

   Insert

   crystallin zeta (CRYZ Human)
   

   Use

   Tags

   FLAG and tGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Cerulean(159-238) in pcDNAI/Amp

   Plasmid

   #60898

   Purpose

   This is a vector that attaches Cerulean(159-238) to the N-terminus of a protein.

   Depositor

   Catherine Berlot

   Article

   Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

   Insert

   Cerulean(159-238)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains N164H substitution

   Promoter

   CMV

   Available Since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. CFP(1-158)-gamma-1 in pcDNAI/Amp

   Plasmid

   #55615

   Purpose

   An amino-terminal CFP fragment was fused to Ggamma-1. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.

   Depositor

   Catherine Berlot

   Article

   Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

   Insert

   CFP(1-158)-gamma-1 (GNGT1 Bovine, Aequorea victoria)
   

   Use

   Tags

   CFP(1-158) was fused to the amino terminus of Gga…

   Expression

   Mammalian

   Mutation

   Ggamma-1 was amplified by PCR, which added a BamH…

   Promoter

   CMV

   Available Since

   Aug. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. CFP(1-158)-gamma-2 in pcDNAI/Amp

   Plasmid

   #55616

   Purpose

   An amino-terminal CFP fragment was fused to Ggamma-2. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.

   Depositor

   Catherine Berlot

   Article

   Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

   Insert

   CFP (1-158)-gamma-2 (Gng2 Mouse, Aequorea victoria)
   

   Use

   Tags

   CFP(1-158) was fused to the amino terminus of Gga…

   Expression

   Mammalian

   Mutation

   Ggamma-2 was amplified by PCR, which added a BamH…

   Promoter

   CMV

   Available Since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA4/TO-bio-myc-NES-EGFP

   Plasmid

   #112712

   Purpose

   For tetracycline-inducible expression of bio-myc-NES-EGFP in mammalian cells

   Depositor

   Daniel Schoenberg

   Article

   Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

   Insert

   EGFP
   

   Use

   Tags

   HIV Rev nuclear localization signal (NES), biotin…

   Expression

   Mammalian

   Mutation

   Nucleotide sequence optimized for synthetic gene …

   Promoter

   CMV with Tet repressor binding sites

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)mGAT1-577-CFP-578CT

   Plasmid

   #41682

   Depositor

   Henry Lester

   Article

   Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

   Insert

   Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   “Monomerizing” CFP A206K mutation; CFP inserted d…

   Promoter

   CMV

   Available Since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+)mGAT1-577-YFP-578CT

   Plasmid

   #41681

   Depositor

   Henry Lester

   Article

   Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

   Insert

   Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   “Monomerizing” YFP A206K mutation; YFP inserted d…

   Promoter

   CMV

   Available Since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.2 mir-1-1 reporter hsa-mir17~20

   Plasmid

   #46671

   Depositor

   David Bartel

   Article

   Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

   Insert

   hsa-mir17~20 (MIR17 Human)
   

   Use

   RNAi

   Tags

   V5 and hsa-mir-1-1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-Lyn-FRB-Venus-173-N

    Plasmid

    #87615

    Purpose

    FRB-tagged N-terminal Venus (YFP) fragment for BiFC; localizes to plasma membrane.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    Lyn-FRB-Venus173-N
    

    Use

    Tags

    N-terminal targeting sequence from Lyn kinase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-FLAG/MKRN1(110-482)

    Plasmid

    #78754

    Purpose

    To overexpress MKRN1(110-482) in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

    Insert

    MKRN1 (MKRN1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    110-482

    Promoter

    CMV

    Available Since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5 FRT TO Myc ArC K72R

    Plasmid

    #59811

    Purpose

    Allows the integration of Myc ArC K72R in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

    Insert

    Aurora C (AURKC Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    K72R, kinase mutant

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available Since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. Human NCKX2-AS-short-FlagTag (pcDNA3.1+)

    Plasmid

    #75202

    Purpose

    Mammalian expression of SLC24A2

    Depositor

    Jonathan Lytton

    Article

    Visser et al J Biol Chem. 2007 Feb 16;282(7):4453-62.

    Insert

    SLC24A2 (SLC24A2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    alternatively spliced exon absent

    Promoter

    CMV

    Available Since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3 Flag MFF iso4 S172A

    Plasmid

    #74393

    Purpose

    Flag tagged human S172A MFF isoform 4 mutant

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser 172 Ala (numbering based on MFF isoform 1)

    Promoter

    Available Since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.1-FLAG-SBP-STAG2 (S1215X)

    Plasmid

    #73970

    Purpose

    STAG2 cDNA (CCDS43990) S1215X

    Depositor

    Todd Waldman

    Article

    Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

    Insert

    STAG2 (STAG2 Human)
    

    Use

    Tags

    FLAG-SBP

    Expression

    Mammalian

    Mutation

    S1215X

    Promoter

    Available Since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1-FLAG-SBP-STAG2 (Q1117X)

    Plasmid

    #73968

    Purpose

    STAG2 cDNA (CCDS43990) Q1117X

    Depositor

    Todd Waldman

    Article

    Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

    Insert

    STAG2 (STAG2 Human)
    

    Use

    Tags

    FLAG-SBP

    Expression

    Mammalian

    Mutation

    Q1117X

    Promoter

    Available Since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3.1-FLAG-SBP-STAG2 (S1075X)

    Plasmid

    #73967

    Purpose

    STAG2 cDNA (CCDS43990) S1075X

    Depositor

    Todd Waldman

    Article

    Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

    Insert

    STAG2 (STAG2 Human)
    

    Use

    Tags

    FLAG-SBP

    Expression

    Mammalian

    Mutation

    S1075X

    Promoter

    Available Since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3.1-FLAG-SBP-STAG2 (S653X)

    Plasmid

    #73965

    Purpose

    STAG2 cDNA (CCDS43990) S653X

    Depositor

    Todd Waldman

    Article

    Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

    Insert

    STAG2 (STAG2 Human)
    

    Use

    Tags

    FLAG-SBP

    Expression

    Mammalian

    Mutation

    S653X

    Promoter

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

19. integrin alpha9 / alpha4 pcDNA1 neo

    Plasmid

    #13587

    Depositor

    Dean Sheppard

    Article

    Young et al Mol Biol Cell. 2001 Oct . 12(10):3214-25

    Insert

    integrin alpha 9 / alpha 4 (ITGA9 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Integrin alpha 9 chimera containing the cytoplasm…

    Promoter

    Available Since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.2 mir-1-1 reporter mir16-1.2

    Plasmid

    #46681

    Purpose

    Expresses a mutant version of the wild-type miR-16-1 in mammalian cells

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir16-1.2 (MIR16-1 Human, Synthetic)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    see sequence and publication

    Promoter

    CMV

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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