We narrowed to 18,492 results for: REV

1. Cas9-GFP_sg_mAMPKa2

   Plasmid

   #79005

   Purpose

   Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 2.

   Depositor

   Reuben Shaw

   Article

   Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

   Insert

   Prkaa2 (Prkaa2 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pCAX APLP1

   Plasmid

   #30141

   Depositor

   Tracy Young-Pearse, Dennis Selkoe

   Article

   Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

   Insert

   Amyloid Precursor Like Protein 1 (APLP1 Human)
   

   Expression

   Mammalian

   Available Since

   July 29, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pMT010

   Plasmid

   #64762

   Purpose

   Recombination-Induced Tag Exchange (RITE) Cassette, HA to a 2xFLAG epitope tag, Hygro selection

   Depositor

   Fred van Leeuwen

   Article

   Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

   Insert

   RITE cassette
   

   Use

   Rite cassette

   Promoter

   none

   Available Since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMT006

   Plasmid

   #64761

   Purpose

   Recombination-Induced Tag Exchange (RITE) Cassette, 2xFLAG to an HA-6xHIS epitope tag, Hygro selection

   Depositor

   Fred van Leeuwen

   Article

   Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

   Insert

   RITE cassette
   

   Use

   Rite cassette

   Promoter

   none

   Available Since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCRCT

   Plasmid

   #60621

   Purpose

   Plasmid encoding iCas9, tracrRNA and crRNAs

   Depositor

   Huimin Zhao

   Article

   Bao et al ACS Synth Biol. 2014 Sep 19.

   Inserts

   iCas9
   tracrRNA
   

   Use

   CRISPR

   Tags

   FLAG and SV40 NLS

   Expression

   Yeast

   Mutation

   changed Aspartate 147 to Tyrosine, Proline 411 to…

   Promoter

   RPR1p and TEF1p

   Available Since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTA-Mob 2.0

   Plasmid

   #149662

   Purpose

   Conjugation helper plasmid for use in cis (self-transfer) and trans (transfer of plasmids with RK2/RP4 origin of transfer) mobilization to prokaryotic and eukaryotic cells.

   Depositor

   Bogumil Karas

   Article

   Soltysiak et al Int J Mol Sci. 2019 Oct 21;20(20). pii:

   Type

   Empty backbone

   Use

   Synthetic Biology; Conjugation helper plasmid

   Expression

   Bacterial

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. 2Bc-T MBP_BoMoC(ed)_W403A_F753A_6xH

   Plasmid

   #185713

   Purpose

   Recombinant B. mori truncated R2 RT expression

   Depositor

   Kathleen Collins

   Article

   Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

   Insert

   B. mori truncated R2 RT
   

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Mutation

   Changed W403 and F753 to A

   Promoter

   T7

   Available Since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pS1643 (pFA6-NATMX-CDC42pr-yGFP1-10)

   Plasmid

   #86420

   Purpose

   N-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter

   Depositor

   Sue Jaspersen

   Article

   Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

   Insert

   CDC42pr-GFP1-10
   

   Tags

   GFP1-10

   Expression

   Yeast

   Available Since

   Feb. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. SpdCas9 VPR

   Plasmid

   #188509

   Purpose

   Expresses FLAG tagged SpdCas9 VPR

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A/H840A

   Promoter

   EF1a

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMCB306-GFP-Rab29

    Plasmid

    #198472

    Purpose

    lentiviral expression of GFP-Rab29

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    GFP-Rab29 (RAB29 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    May 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFvl160

    Plasmid

    #64753

    Purpose

    Recombination-Induced Tag Exchange (RITE) Cassette, 3xHA to a 3xT7 epitope tag, Hygro selection

    Depositor

    Fred van Leeuwen

    Article

    Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

    Insert

    RITE cassette
    

    Use

    Rite cassette

    Promoter

    none

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pECIA2

    Plasmid

    #47032

    Purpose

    This is a Gateway destination vector for secreted expression in Drosophila cell culture by induction via CuSO4. Inserts will be C-terminally tagged with Fc, V5 and hexahistidine tags.

    Depositor

    Chris Garcia

    Article

    Ozkan et al Cell. 2013 Jul 3;154(1):228-39. doi: 10.

    Type

    Empty backbone

    Use

    Secreted expression in drosophila culture

    Tags

    Fc(human IgG1), HRV 3C Protease Cleavage site, He…

    Expression

    Insect

    Promoter

    Metallothionein (Copper-Inducible)

    Available Since

    Aug. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDO068

    Plasmid

    #198788

    Purpose

    Drives specific expression in the BAG neurons

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    Pgcy-33-GAL4-SK(DBD)-VP64
    

    Expression

    Worm

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Nrxn3b-Nav1.6-MKII-f-hLOV1-TEVcs(ENLYFQ/M)-tTA-VP16

    Plasmid

    #163031

    Purpose

    FLiCRE TF with fast kinetics for neuronal expression

    Depositor

    Alice Ting

    Article

    Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

    Insert

    Nrxn3b-MKII-f-hLOV1-TEVcs-tTA
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Synapsin

    Available Since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PCS4 3XFLAG-PPARgamma1

    Plasmid

    #78769

    Purpose

    To overexpress PPARgamma1 in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Kim et al Cell Death Differ. 2014 Apr;21(4):594-60

    Insert

    PPAR_1 (Pparg Mouse)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV IE94

    Available Since

    June 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSB819-PKR-hum

    Plasmid

    #20030

    Depositor

    Harmit Malik

    Article

    Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

    Insert

    Protein kinase R (EIF2AK2 Human)
    

    Expression

    Yeast

    Available Since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR-nlsABACUS2-400n

    Plasmid

    #203726

    Purpose

    Gateway entry vector for the the nlsABACUS2-400n biosensor for (+)-Abscisic acid

    Depositor

    Alexander Jones

    Article

    Rowe et al Nat Plants. 2023 Jun 26. doi: 10.1038/s4

    Insert

    nlsABACUS2-400n
    

    Use

    Synthetic Biology

    Tags

    Myc

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HDAC5 Flag

    Plasmid

    #13822

    Purpose

    Mammalian expression of human histone deacetylase 5 with flag tag

    Depositor

    Eric Verdin

    Article

    Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

    Insert

    HDAC5 (HDAC5 Human)
    

    Tags

    flag

    Expression

    Mammalian

    Available Since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. Puro-iNgn3

    Plasmid

    #75337

    Purpose

    doxycycline inducible Ngn3 over-expression

    Depositor

    Danwei Huangfu

    Article

    Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

    Insert

    neurogenin 3 (Neurog3 Mouse)
    

    Expression

    Mammalian

    Promoter

    TRE-tight

    Available Since

    May 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEasyCas9

    Plasmid

    #184909

    Purpose

    Expresses the Streptococcus pyogenes Cas9 protein

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Bacterial and Yeast

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. p-AAV-sh[control]

    Plasmid

    #75438

    Purpose

    control shRNA vector

    Depositor

    Edward Burton

    Article

    Zharikov et al J Clin Invest. 2015 Jul 1;125(7):2721-35

    Insert

    GFP
    

    Use

    AAV and RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pX459-HypaCas9-mR2-AAVS1_sgRNA

    Plasmid

    #183890

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and sgRNA targeting the AAVS1 in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    AAVS1 sgRNA spacer (AAVS1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCB578

    Plasmid

    #141095

    Purpose

    E. coli-Lactobacilli shuttle vector containing SpCas9, tracrRNA, and a repeat-spacer-repeat array

    Depositor

    Chase Beisel

    Article

    Leenay et al Biotechnol J. 2019 Mar;14(3):e1700583. d

    Inserts

    Cas9 and tracrRNA
    repeat-spacer-repeat array
    

    Expression

    Bacterial

    Available Since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Stx4-pmCitrine

    Plasmid

    #92422

    Purpose

    Expression of syntaxin-4 C-terminally conjugated to mCitrine.

    Depositor

    Geert van den Bogaart

    Article

    Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

    Insert

    Stx4 (Stx4 Rat)
    

    Tags

    mCitrine

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. plRL61

    Plasmid

    #163633

    Purpose

    Optimized M. smegmatis CRISPRi plasmid; contains an L5 attP region (no L5 Integrase) that allows for integration when co-transformed with an L5 Integrase delivery plasmid (e.g. plRL19, ID #163634).

    Depositor

    Jeremy Rock

    Article

    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    Tet-On

    Available Since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. TLCV2-DelGFP

    Plasmid

    #133302

    Purpose

    EGFP reporter was removed in the TLCV2 vector so it could be used for inducible gene CRISPR knockout in cell lines with an eGFP reporter.

    Depositor

    Jingshi Shen

    Article

    Wang et al J Biol Chem. 2019 Dec 27;294(52):19988-1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. beta-arrestin2 mYFP

    Plasmid

    #36917

    Depositor

    Robert Lefkowitz

    Article

    Violin et al J Biol Chem. 2006 Jul 21;281(29):20577-8

    Insert

    beta-arrestin2 (Arrb2 Rat)
    

    Tags

    mYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CMV500 A-FOS

    Plasmid

    #33353

    Depositor

    Charles Vinson

    Article

    Ahn et al Mol Cell Biol. 1998 Feb . 18(2):967-77.

    Insert

    FOS (Fos Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Dominant Negative of c-Jun; heterodimerizes and i…

    Promoter

    CMV

    Available Since

    Dec. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. p306-BrdU-Inc

    Plasmid

    #71792

    Purpose

    BrdU-incorporating plasmid with selectable marker URA3

    Depositor

    Oscar Aparicio

    Article

    Viggiani et al Yeast. 2006 Oct-Nov;23(14-15):1045-51.

    Inserts

    Thymidine Kinase
    ENT1 (SLC29A1 Human)
    

    Expression

    Yeast

    Promoter

    ADH1 and GPD

    Available Since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1(Hygro) TagBFP-hNucleolin

    Plasmid

    #182592

    Purpose

    expresses bfp-hNucleolin in mammalian cells

    Depositor

    Agnel Sfeir

    Article

    Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

    Insert

    hNucleolin (NCL Human)
    

    Tags

    BFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge